Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum

Carbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other...

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Autores: García-Rivera, C, Sánchez-Bautista, A, Parra-Grande, M, Ricart-Silvestre, A, Ventero, MP, Tyshkovska, I, Merino, E, Díaz, JCR
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositorio:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p10299
Acesso em linha:https://isabial.portalinvestigacion.com/publicaciones10299
https://www.mdpi.com/2079-6382/13/7/663
Access Level:acceso abierto
Palavra-chave:cefiderocol
inoculum
Pseudomonas aeruginosa
in vitro activity
antibiogram
resistance
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spelling Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial InoculumGarcía-Rivera, CSánchez-Bautista, AParra-Grande, MRicart-Silvestre, AVentero, MPTyshkovska, IMerino, EDíaz, JCRcefiderocolinoculumPseudomonas aeruginosain vitro activityantibiogramresistanceCarbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other drugs. However, concerns regarding the emergence of drug-resistant strains persist. This study, conducted with 109 carbapenem-resistant Pseudomonas aeruginosa strains from the Spanish Hospital (Dr. Balmis, Alicante). The study evaluated susceptibility to cefiderocol in comparison to alternative antibiotics and including their susceptibility to bacterial inoculum, while assessing various testing methods. Our findings revealed high susceptibility to cefiderocol against carbapenem-resistant strains, with only 2 of 109 strains exhibiting resistance. Comparative analysis demonstrated superiority of cefiderocol towards alternative antibiotics. Both the E-test and disk-diffusion methods showed 100% concordance with the microdilution method in classifying strains as susceptible or resistant. However, 4.6% (5/109) of disc zone diameters fell within the technical uncertainty zone, so the E-test technique was found to be more useful in routine clinical practice. Additionally, escalating bacterial inoculum correlated with decreases in vitro activity, so this parameter should be adjusted very carefully in in vivo studies. This study underscores cefiderocol's potential as a therapeutic option for carbapenem-resistant Pseudomonas aeruginosa infections. However, the emergence of drug-resistant strains emphasizes the critical need for a wise use of antibiotics and a continuous monitoring of resistance to antibiotics. Based on our in vitro data, further investigation concerning the impact of bacterial inoculum on drug efficacy is warranted in order to detect resistance mechanisms and optimize treatment strategies, thereby mitigating the risk of resistance.MDPI2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones10299https://www.mdpi.com/2079-6382/13/7/663Antibiotics-BaselISSN: 20796382reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p102992026-06-12T10:20:37Z
dc.title.none.fl_str_mv Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
title Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
spellingShingle Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
García-Rivera, C
cefiderocol
inoculum
Pseudomonas aeruginosa
in vitro activity
antibiogram
resistance
title_short Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
title_full Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
title_fullStr Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
title_full_unstemmed Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
title_sort Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> Strains: Influence of Bacterial Inoculum
dc.creator.none.fl_str_mv García-Rivera, C
Sánchez-Bautista, A
Parra-Grande, M
Ricart-Silvestre, A
Ventero, MP
Tyshkovska, I
Merino, E
Díaz, JCR
author García-Rivera, C
author_facet García-Rivera, C
Sánchez-Bautista, A
Parra-Grande, M
Ricart-Silvestre, A
Ventero, MP
Tyshkovska, I
Merino, E
Díaz, JCR
author_role author
author2 Sánchez-Bautista, A
Parra-Grande, M
Ricart-Silvestre, A
Ventero, MP
Tyshkovska, I
Merino, E
Díaz, JCR
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cefiderocol
inoculum
Pseudomonas aeruginosa
in vitro activity
antibiogram
resistance
topic cefiderocol
inoculum
Pseudomonas aeruginosa
in vitro activity
antibiogram
resistance
description Carbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other drugs. However, concerns regarding the emergence of drug-resistant strains persist. This study, conducted with 109 carbapenem-resistant Pseudomonas aeruginosa strains from the Spanish Hospital (Dr. Balmis, Alicante). The study evaluated susceptibility to cefiderocol in comparison to alternative antibiotics and including their susceptibility to bacterial inoculum, while assessing various testing methods. Our findings revealed high susceptibility to cefiderocol against carbapenem-resistant strains, with only 2 of 109 strains exhibiting resistance. Comparative analysis demonstrated superiority of cefiderocol towards alternative antibiotics. Both the E-test and disk-diffusion methods showed 100% concordance with the microdilution method in classifying strains as susceptible or resistant. However, 4.6% (5/109) of disc zone diameters fell within the technical uncertainty zone, so the E-test technique was found to be more useful in routine clinical practice. Additionally, escalating bacterial inoculum correlated with decreases in vitro activity, so this parameter should be adjusted very carefully in in vivo studies. This study underscores cefiderocol's potential as a therapeutic option for carbapenem-resistant Pseudomonas aeruginosa infections. However, the emergence of drug-resistant strains emphasizes the critical need for a wise use of antibiotics and a continuous monitoring of resistance to antibiotics. Based on our in vitro data, further investigation concerning the impact of bacterial inoculum on drug efficacy is warranted in order to detect resistance mechanisms and optimize treatment strategies, thereby mitigating the risk of resistance.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones10299
https://www.mdpi.com/2079-6382/13/7/663
url https://isabial.portalinvestigacion.com/publicaciones10299
https://www.mdpi.com/2079-6382/13/7/663
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Antibiotics-Basel
ISSN: 20796382
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
reponame_str r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
collection r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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