Profiling of the pflD mutation suggests it is a neutral passenger
(A) Mutation rates for 2-DOS aminoglycoside resistance in the original K43N first-step mutant carrying the pflD mutation and two independently isolated clones confirmed to carry only the intended K43N rpsL mutation. Values represent the mutation rate ± 95% confidence interval, estimated from five pa...
| Autores: | , , , |
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| Formato: | conjunto de datos |
| Fecha de publicación: | 2025 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:dnet:digitalcsic_::6f01ba1407921574d1cb0d230afd35ba |
| Acesso em linha: | http://hdl.handle.net/10261/426254 |
| Access Level: | acceso abierto |
| Palavra-chave: | Systems involving single Smaller effect sizes Promote evolutionary robustness Genotypes deviate markedly Genome sequencing reveals Electron transport chain Combat multidrug resistance Investigate whether common biophysics genetics microbiology biotechnology evolutionary biology ecology infectious diseases Biochemistry |
| Resumo: | (A) Mutation rates for 2-DOS aminoglycoside resistance in the original K43N first-step mutant carrying the pflD mutation and two independently isolated clones confirmed to carry only the intended K43N rpsL mutation. Values represent the mutation rate ± 95% confidence interval, estimated from five parallel replicate cultures. Selection was performed at the wild-type’s Mutant Prevention Concentration (MPC), as in Fig 1. The small differences in mutation rates are not significant (pairwise likelihood ratio tests, Benjamini-Hochberg corrected). (B) Growth curves in the absence of antibiotics for the original K43N first-step mutant carrying the pflD mutation and the two clones confirmed to carry only the K43N mutation in rpsL. Details as in S4 Fig. |
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