A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus

© 2021 The Authors.

Detalhes bibliográficos
Autores: Kabeya, Naoki, Ogino, Masanari, Ushio, H., Haga, Yutaka, Satoh, Shuichi, Navarro, Juan Carlos, Monroig, Óscar
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/239611
Acesso em linha:http://hdl.handle.net/10261/239611
Access Level:acceso abierto
Palavra-chave:Biosynthesis
Polyunsaturated fatty acids
Fatty acyl elongases
Front-end desaturases
Methylend desaturases
Harpacticoid copepods
id ES_e81bd5b195820a1c8fb4f667b8aa34bb
oai_identifier_str oai:digital.csic.es:10261/239611
network_acronym_str ES
network_name_str España
repository_id_str
spelling A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicusKabeya, NaokiOgino, MasanariUshio, H.Haga, YutakaSatoh, ShuichiNavarro, Juan CarlosMonroig, ÓscarBiosynthesisPolyunsaturated fatty acidsFatty acyl elongasesFront-end desaturasesMethylend desaturasesHarpacticoid copepods© 2021 The Authors.The long-standing paradigm establishing that global production of Omega-3 (n–3) long-chain polyunsaturated fatty acids (LC-PUFA) derived almost exclusively from marine single-cell organisms, was recently challenged by the discovery that multiple invertebrates possess methyl-end (or ωx) desaturases, critical enzymes enabling the biosynthesis of n–3 LC-PUFA. However, the question of whether animals with ωx desaturases have complete n–3 LC-PUFA biosynthetic pathways and hence can contribute to the production of these compounds in marine ecosystems remained unanswered. In the present study, we investigated the complete enzymatic complement involved in the n–3 LC-PUFA biosynthesis in Tigriopus californicus, an intertidal harpacticoid copepod. A total of two ωx desaturases, five front-end desaturases and six fatty acyl elongases were successfully isolated and functionally characterized. The T. californicus ωx desaturases enable the de novo biosynthesis of C18 PUFA such as linoleic and α-linolenic acids, as well as several n–3 LC-PUFA from n–6 substrates. Functions demonstrated in front-end desaturases and fatty acyl elongases unveiled various routes through which T. californicus can biosynthesize the physiologically important arachidonic and eicosapentaenoic acids. Moreover, T. californicus possess a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid via the ‘Δ4 pathway’. In conclusion, harpacticoid copepods such as T. californicus have complete n–3 LC-PUFA biosynthetic pathways and such capacity illustrates major roles of these invertebrates in the provision of essential fatty acids to upper trophic levels.This study was partly funded through the project IMPROMEGA of the Ministry of Science, Innovation and Universities, Spanish Government (grant no. RTI2018-095119-B-I00, MCIU/AEI/FEDER, UE). N.K. was supported by the JSPS KAKENHI grant no. JP19K15908.Peer reviewedRoyal Society (Great Britain)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Japan Society for the Promotion of ScienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/239611reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00RTI2018-095119-B-I00/AEI/10.13039/501100011033https://doi.org/10.1098/rsob.200402Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2396112026-05-22T06:33:51Z
dc.title.none.fl_str_mv A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
title A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
spellingShingle A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
Kabeya, Naoki
Biosynthesis
Polyunsaturated fatty acids
Fatty acyl elongases
Front-end desaturases
Methylend desaturases
Harpacticoid copepods
title_short A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
title_full A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
title_fullStr A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
title_full_unstemmed A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
title_sort A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus
dc.creator.none.fl_str_mv Kabeya, Naoki
Ogino, Masanari
Ushio, H.
Haga, Yutaka
Satoh, Shuichi
Navarro, Juan Carlos
Monroig, Óscar
author Kabeya, Naoki
author_facet Kabeya, Naoki
Ogino, Masanari
Ushio, H.
Haga, Yutaka
Satoh, Shuichi
Navarro, Juan Carlos
Monroig, Óscar
author_role author
author2 Ogino, Masanari
Ushio, H.
Haga, Yutaka
Satoh, Shuichi
Navarro, Juan Carlos
Monroig, Óscar
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Japan Society for the Promotion of Science
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biosynthesis
Polyunsaturated fatty acids
Fatty acyl elongases
Front-end desaturases
Methylend desaturases
Harpacticoid copepods
topic Biosynthesis
Polyunsaturated fatty acids
Fatty acyl elongases
Front-end desaturases
Methylend desaturases
Harpacticoid copepods
description © 2021 The Authors.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/239611
url http://hdl.handle.net/10261/239611
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00
RTI2018-095119-B-I00/AEI/10.13039/501100011033
https://doi.org/10.1098/rsob.200402
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society (Great Britain)
publisher.none.fl_str_mv Royal Society (Great Britain)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422912941850624
score 15,812455

Registos relacionados