Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

This is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9

Detalhes bibliográficos
Autores: García Mendiola, Tania, Elosegui, Cristina Garcia, Bravo, Iria, Pariente Alonso, Félix, Jacobo-Martin, Alejandra, Navío Bernabeu, Cristina, Rodriguez, Isabel, Wannemacher, Reinhold, Lorenzo Abad, Encarnación
Tipo de documento: artigo
Data de publicação:2019
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/689194
Acesso em linha:http://hdl.handle.net/10486/689194
https://dx.doi.org/10.1007/s00604-019-3386-9
Access Level:Acceso aberto
Palavra-chave:Cancer
DNA sensing
Fluorescent assay
Fluorescent carbon nanodots
Genetic diseases
Mutations
Rapid assay
Química
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spelling Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutationsGarcía Mendiola, TaniaElosegui, Cristina GarciaBravo, IriaPariente Alonso, FélixJacobo-Martin, AlejandraNavío Bernabeu, CristinaRodriguez, IsabelWannemacher, ReinholdLorenzo Abad, EncarnaciónCancerDNA sensingFluorescent assayFluorescent carbon nanodotsGenetic diseasesMutationsRapid assayQuímicaThis is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samplesCAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSOSpringer VerlagDepartamento de Química Analítica y Análisis InstrumentalFacultad de Ciencias20192019-05-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/689194https://dx.doi.org/10.1007/s00604-019-3386-9reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6891942026-06-23T12:46:27Z
dc.title.none.fl_str_mv Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
title Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
spellingShingle Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
García Mendiola, Tania
Cancer
DNA sensing
Fluorescent assay
Fluorescent carbon nanodots
Genetic diseases
Mutations
Rapid assay
Química
title_short Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
title_full Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
title_fullStr Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
title_full_unstemmed Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
title_sort Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
dc.creator.none.fl_str_mv García Mendiola, Tania
Elosegui, Cristina Garcia
Bravo, Iria
Pariente Alonso, Félix
Jacobo-Martin, Alejandra
Navío Bernabeu, Cristina
Rodriguez, Isabel
Wannemacher, Reinhold
Lorenzo Abad, Encarnación
author García Mendiola, Tania
author_facet García Mendiola, Tania
Elosegui, Cristina Garcia
Bravo, Iria
Pariente Alonso, Félix
Jacobo-Martin, Alejandra
Navío Bernabeu, Cristina
Rodriguez, Isabel
Wannemacher, Reinhold
Lorenzo Abad, Encarnación
author_role author
author2 Elosegui, Cristina Garcia
Bravo, Iria
Pariente Alonso, Félix
Jacobo-Martin, Alejandra
Navío Bernabeu, Cristina
Rodriguez, Isabel
Wannemacher, Reinhold
Lorenzo Abad, Encarnación
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Analítica y Análisis Instrumental
Facultad de Ciencias
dc.subject.none.fl_str_mv Cancer
DNA sensing
Fluorescent assay
Fluorescent carbon nanodots
Genetic diseases
Mutations
Rapid assay
Química
topic Cancer
DNA sensing
Fluorescent assay
Fluorescent carbon nanodots
Genetic diseases
Mutations
Rapid assay
Química
description This is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-05-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/689194
https://dx.doi.org/10.1007/s00604-019-3386-9
url http://hdl.handle.net/10486/689194
https://dx.doi.org/10.1007/s00604-019-3386-9
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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