2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8

Topic name Thioredoxin; Oxidation Reduction Reaction; Enzyme Prominence percentile 82.555

Detalhes bibliográficos
Autores: Hernández, María Luisa, Jiménez-López, Julia, Cejudo, Francisco Javier, Pérez-Ruiz, Juan Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/375045
Acesso em linha:http://hdl.handle.net/10261/375045
https://api.elsevier.com/content/abstract/scopus_id/85195028840
Access Level:acceso abierto
Palavra-chave:Biological Sciences
Botany
Plant Development
Plant Evolution
Plant Physiology
Plant Reproduction and Propagation
Plant sciences and Forestry
Science and Mathematics
Books
Journals
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oai_identifier_str oai:digital.csic.es:10261/375045
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
title 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
spellingShingle 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
Hernández, María Luisa
Biological Sciences
Botany
Plant Development
Plant Evolution
Plant Physiology
Plant Reproduction and Propagation
Plant sciences and Forestry
Science and Mathematics
Books
Journals
title_short 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
title_full 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
title_fullStr 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
title_full_unstemmed 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
title_sort 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8
dc.creator.none.fl_str_mv Hernández, María Luisa
Jiménez-López, Julia
Cejudo, Francisco Javier
Pérez-Ruiz, Juan Manuel
author Hernández, María Luisa
author_facet Hernández, María Luisa
Jiménez-López, Julia
Cejudo, Francisco Javier
Pérez-Ruiz, Juan Manuel
author_role author
author2 Jiménez-López, Julia
Cejudo, Francisco Javier
Pérez-Ruiz, Juan Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Kansas Lipidomics Research Center
National Science Foundation (US)
National Institutes of Health (US)
National Institute of Food and Agriculture (US)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Kansas State University
Agencia Estatal de Investigación (España)
Hernández, María Luisa [0000-0003-2160-1764]
Jiménez López, Julia [0000-0003-4659-4070]
Cejudo, Francisco Javier [0000-0002-3936-5491]
Pérez Ruiz, Juan Manuel [0000-0002-9992-497X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biological Sciences
Botany
Plant Development
Plant Evolution
Plant Physiology
Plant Reproduction and Propagation
Plant sciences and Forestry
Science and Mathematics
Books
Journals
topic Biological Sciences
Botany
Plant Development
Plant Evolution
Plant Physiology
Plant Reproduction and Propagation
Plant sciences and Forestry
Science and Mathematics
Books
Journals
description Topic name Thioredoxin; Oxidation Reduction Reaction; Enzyme Prominence percentile 82.555
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/375045
https://api.elsevier.com/content/abstract/scopus_id/85195028840
url http://hdl.handle.net/10261/375045
https://api.elsevier.com/content/abstract/scopus_id/85195028840
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115156GB-I00
Hernández, María Luisa; Jiménez-López, Julia; Cejudo, Francisco Javier; Pérez-Ruiz, Juan Manuel; 2024; Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8 [Dataset]; ]Oxford University Press; https://doi.org/10.1093/plphys/kiae102/s1
https://doi.org/10.1093/plphys/kiae102
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422849680211968
spelling 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8Hernández, María LuisaJiménez-López, JuliaCejudo, Francisco JavierPérez-Ruiz, Juan ManuelBiological SciencesBotanyPlant DevelopmentPlant EvolutionPlant PhysiologyPlant Reproduction and PropagationPlant sciences and ForestryScience and MathematicsBooksJournalsTopic name Thioredoxin; Oxidation Reduction Reaction; Enzyme Prominence percentile 82.555Chemicals and CAS Registry Numbers acyl coenzyme A desaturase 9014-34-0 peroxiredoxin 207137-51-7 Arabidopsis Proteins Fatty Acid Desaturases omega-3 fatty acid desaturase PeroxiredoxinsFatty acid unsaturation levels affect chloroplast function and plant acclimation to environmental cues. However, the regulatory mechanism(s) controlling fatty acid unsaturation in thylakoid lipids is poorly understood. Here, we have investigated the connection between chloroplast redox homeostasis and lipid metabolism by focusing on 2-Cys peroxiredoxins (Prxs), which play a central role in balancing the redox state within the organelle. The chloroplast redox network relies on NADPH-dependent thioredoxin reductase C (NTRC), which controls the redox balance of 2-Cys Prxs to maintain the reductive activity of redox-regulated enzymes. Our results show that Arabidopsis (Arabidopsis thaliana) mutants deficient in 2-Cys Prxs contain decreased levels of trienoic fatty acids, mainly in chloroplast lipids, indicating that these enzymes contribute to thylakoid membrane lipids unsaturation. This function of 2-Cys Prxs is independent of NTRC, the main reductant of these enzymes, hence 2-Cys Prxs operates beyond the classic chloroplast regulatory redox system. Moreover, the effect of 2-Cys Prxs on lipid metabolism is primarily exerted through the prokaryotic pathway of glycerolipid biosynthesis and fatty acid desaturase 8 (FAD8). While 2-Cys Prxs and FAD8 interact in leaf membranes as components of a large protein complex, the levels of FAD8 were markedly decreased when FAD8 is overexpressed in 2-Cys Prxs-deficient mutant backgrounds. These findings reveal a function for 2-Cys Prxs, possibly acting as a scaffold protein, affecting the unsaturation degree of chloroplast membranes.We acknowledge Carlos Parejo and Alicia Orea from Chromatography and Advanced Optical Microscopy facilities (Instituto de Bioquímica Vegetal y Fotosíntesis) for technical assistance. The lipidomic analyses described in this work were performed at the Kansas Lipidomics Research Center Analytical Laboratory. Instrument acquisition and lipidomics method development were supported by the National Science Foundation (including support from the Major Research Instrumentation program; most recent award DBI-1726527), K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20GM103418), USDA National Institute of Food and Agriculture (Hatch/Multi-State project 1013013), and Kansas State University. The WT/FAD8–YFP–HA transgenic line and fad7 and fad8 mutants were kindly provided by Dr. Miguel Alfonso, Estación Experimental Aula Dei, CSIC, Spain. J.J-L. was supported by a postdoctoral contract from Consejo Superior de Investigaciones Científicas (Spain) and M.L.H. by a postdoctoral contract from the VIPPI-Universidad de Sevilla.Peer reviewedOxford University PressKansas Lipidomics Research CenterNational Science Foundation (US)National Institutes of Health (US)National Institute of Food and Agriculture (US)Consejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia, Innovación y Universidades (España)Kansas State UniversityAgencia Estatal de Investigación (España)Hernández, María Luisa [0000-0003-2160-1764]Jiménez López, Julia [0000-0003-4659-4070]Cejudo, Francisco Javier [0000-0002-3936-5491]Pérez Ruiz, Juan Manuel [0000-0002-9992-497X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionaplication/pdfhttp://hdl.handle.net/10261/375045https://api.elsevier.com/content/abstract/scopus_id/85195028840reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115156GB-I00Hernández, María Luisa; Jiménez-López, Julia; Cejudo, Francisco Javier; Pérez-Ruiz, Juan Manuel; 2024; Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8 [Dataset]; ]Oxford University Press; https://doi.org/10.1093/plphys/kiae102/s1https://doi.org/10.1093/plphys/kiae102Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3750452026-05-22T06:33:51Z
score 15.198674

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