Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species

The elevated concentration of hydrogen peroxide (H2O2) due to natural and anthropogenic causes is a significant bane to marine and coastal organisms, as it has a prolonged half-life and can inject across lipid bilayers and interfere with aquatic redox processes. The present assessment aimed to deter...

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Autores: Alam, Rushna, Ehiguese, Friday O., Vitale, Dyana, Martín-Díaz, M. Laura
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/350257
Acesso em linha:http://hdl.handle.net/10261/350257
Access Level:Acceso aberto
Palavra-chave:Acute toxicity
Sublethal effects
Biochemical biomarkers
Ruditapes philippinarum
Mytilus galloprovincialis
Reactive oxygen species
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spelling Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve speciesAlam, RushnaEhiguese, Friday O.Vitale, DyanaMartín-Díaz, M. LauraAcute toxicitySublethal effectsBiochemical biomarkersRuditapes philippinarumMytilus galloprovincialisReactive oxygen speciesThe elevated concentration of hydrogen peroxide (H2O2) due to natural and anthropogenic causes is a significant bane to marine and coastal organisms, as it has a prolonged half-life and can inject across lipid bilayers and interfere with aquatic redox processes. The present assessment aimed to determine the acute effect of H2O2 on larval development of the Mediterranean mussel Mytilus galloprovincialis and to observe the oxidative stress (OS) responses, including genotoxicity and neurotoxicity in the clam Ruditapes philippinarum. Two experiments were performed, in which (a) mussel embryos were exposed to the consecutive dilution (0.0, 0.05, 0.5, 5.0, 50.0 and 500.00 μM) of H2O2 for 48 h and (b) adult clams were exposed to similar concentrations - of this reactive oxygen species (ROS) for 21 days. Incremental H2O2 significantly retarded the growth of mussel larvae; the percentage of malformed larvae increased with increasing toxicant concentration (p < 0.01, and r = 0.884). H2O2 also induced oxidative stress (OS) in clams, indicated by the activation/inactivation of antioxidant enzymes (GST, GPx, and GR) with a significant rise in lipid peroxidation (LPO) in the digestive gland tissues of the clams (p < 0.05). No genotoxicity or neurotoxicity on R. philippinarum were evident after chronic exposure. The battery of responses (organismal and cellular parameters) showed embryotoxicity and sublethal effects on bivalves, which confirmed their suitability for assessing the effect of H2O2 mediated OS responses in the marine environment.The first and 2nd authors highly appreciate Erasmus Mundus Master in Water and Coastal Management (WACOMA) consortium for scholarship and funding.Peer reviewedAcademic JournalsUniversidad de CádizConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/350257reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.enceco.2022.01.002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3502572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
title Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
spellingShingle Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
Alam, Rushna
Acute toxicity
Sublethal effects
Biochemical biomarkers
Ruditapes philippinarum
Mytilus galloprovincialis
Reactive oxygen species
title_short Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
title_full Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
title_fullStr Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
title_full_unstemmed Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
title_sort Oxidative stress response to hydrogen peroxide exposure of Mytilus galloprovincialis and Ruditapes philippinarum: Reduced embryogenesis success and altered biochemical response of sentinel marine bivalve species
dc.creator.none.fl_str_mv Alam, Rushna
Ehiguese, Friday O.
Vitale, Dyana
Martín-Díaz, M. Laura
author Alam, Rushna
author_facet Alam, Rushna
Ehiguese, Friday O.
Vitale, Dyana
Martín-Díaz, M. Laura
author_role author
author2 Ehiguese, Friday O.
Vitale, Dyana
Martín-Díaz, M. Laura
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad de Cádiz
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Acute toxicity
Sublethal effects
Biochemical biomarkers
Ruditapes philippinarum
Mytilus galloprovincialis
Reactive oxygen species
topic Acute toxicity
Sublethal effects
Biochemical biomarkers
Ruditapes philippinarum
Mytilus galloprovincialis
Reactive oxygen species
description The elevated concentration of hydrogen peroxide (H2O2) due to natural and anthropogenic causes is a significant bane to marine and coastal organisms, as it has a prolonged half-life and can inject across lipid bilayers and interfere with aquatic redox processes. The present assessment aimed to determine the acute effect of H2O2 on larval development of the Mediterranean mussel Mytilus galloprovincialis and to observe the oxidative stress (OS) responses, including genotoxicity and neurotoxicity in the clam Ruditapes philippinarum. Two experiments were performed, in which (a) mussel embryos were exposed to the consecutive dilution (0.0, 0.05, 0.5, 5.0, 50.0 and 500.00 μM) of H2O2 for 48 h and (b) adult clams were exposed to similar concentrations - of this reactive oxygen species (ROS) for 21 days. Incremental H2O2 significantly retarded the growth of mussel larvae; the percentage of malformed larvae increased with increasing toxicant concentration (p < 0.01, and r = 0.884). H2O2 also induced oxidative stress (OS) in clams, indicated by the activation/inactivation of antioxidant enzymes (GST, GPx, and GR) with a significant rise in lipid peroxidation (LPO) in the digestive gland tissues of the clams (p < 0.05). No genotoxicity or neurotoxicity on R. philippinarum were evident after chronic exposure. The battery of responses (organismal and cellular parameters) showed embryotoxicity and sublethal effects on bivalves, which confirmed their suitability for assessing the effect of H2O2 mediated OS responses in the marine environment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/350257
url http://hdl.handle.net/10261/350257
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.enceco.2022.01.002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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