Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.

Thyroid hormones, in particular 3,5,3′-triiodothyronine or T3, are involved in multiple physiological processes in mammals such as protein, fat and carbohydrate metabolism. However, the metabolic actions of T3 in fish are still not fully elucidated. We therefore tested the effects of T3 on Sparus au...

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Detalhes bibliográficos
Autores: Vargas-Chacoff, Luis, Ruiz-Jarabo, Ignacio, Arjona, Francisco J., Laiz-Carrión, Raúl, Flik, Gert, Klaren, Peter H. M., Mancera, Juan Miguel
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/326232
Acesso em linha:http://hdl.handle.net/10261/326232
Access Level:Acceso aberto
Palavra-chave:3
Centro Oceanográfico de Málaga
Pesquerías
5
3′-Triiodo-l-thyronine (T3)
Energy metabolism
Protein catabolism
Lipid catabolism
Osmoregulation
Sparus aurata
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network_name_str España
repository_id_str
spelling Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.Vargas-Chacoff, LuisRuiz-Jarabo, IgnacioArjona, Francisco J.Laiz-Carrión, RaúlFlik, GertKlaren, Peter H. M.Mancera, Juan Miguel3Centro Oceanográfico de MálagaPesquerías53′-Triiodo-l-thyronine (T3)Energy metabolismProtein catabolismLipid catabolismOsmoregulationSparus aurataThyroid hormones, in particular 3,5,3′-triiodothyronine or T3, are involved in multiple physiological processes in mammals such as protein, fat and carbohydrate metabolism. However, the metabolic actions of T3 in fish are still not fully elucidated. We therefore tested the effects of T3 on Sparus aurata energy metabolism and osmoregulatory system, a hyperthyroid-induced model that was chosen. Fish were implanted with coconut oil depots (containing 0, 2.5, 5.0 and 10.0 μg T3/g body weight) and sampled at day 3 and 6 post-implantation. Plasma levels of free T3 as well as glucose, lactate and triglyceride values increased with increasing doses of T3 at days 3 and 6 post-implantation. Changes in plasma and organ metabolite levels (glucose, glycogen, triglycerides, lactate and total α amino acid) and enzyme activities related to carbohydrate, lactate, amino acid and lipid pathways were detected in organs involved in metabolism (liver) and osmoregulation (gills and kidney). Our data implicate that the liver uses amino acids as an energy source in response to the T3 treatment, increasing protein catabolism and gluconeogenic pathways. The gills, the most important extruder of ammonia, are fuelled not only by amino acids, but also by lactate. The kidney differs significantly in its substrate preference from the gills, as it obtained metabolic energy from lactate but also from lipid oxidation processes. We conclude that in S. aurata lipid catabolism and protein turnover are increased as a consequence of experimentally induced hyperthyroidism, with secondary osmoregulatory effects.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/326232reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Málagahttps://doi.org/10.1016/j.cbpa.2015.09.014Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3262322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
title Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
spellingShingle Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
Vargas-Chacoff, Luis
3
Centro Oceanográfico de Málaga
Pesquerías
5
3′-Triiodo-l-thyronine (T3)
Energy metabolism
Protein catabolism
Lipid catabolism
Osmoregulation
Sparus aurata
title_short Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
title_full Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
title_fullStr Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
title_full_unstemmed Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
title_sort Energy metabolism of hyperthyroid-induced gilthead sea bream Sparus aurata L.
dc.creator.none.fl_str_mv Vargas-Chacoff, Luis
Ruiz-Jarabo, Ignacio
Arjona, Francisco J.
Laiz-Carrión, Raúl
Flik, Gert
Klaren, Peter H. M.
Mancera, Juan Miguel
author Vargas-Chacoff, Luis
author_facet Vargas-Chacoff, Luis
Ruiz-Jarabo, Ignacio
Arjona, Francisco J.
Laiz-Carrión, Raúl
Flik, Gert
Klaren, Peter H. M.
Mancera, Juan Miguel
author_role author
author2 Ruiz-Jarabo, Ignacio
Arjona, Francisco J.
Laiz-Carrión, Raúl
Flik, Gert
Klaren, Peter H. M.
Mancera, Juan Miguel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 3
Centro Oceanográfico de Málaga
Pesquerías
5
3′-Triiodo-l-thyronine (T3)
Energy metabolism
Protein catabolism
Lipid catabolism
Osmoregulation
Sparus aurata
topic 3
Centro Oceanográfico de Málaga
Pesquerías
5
3′-Triiodo-l-thyronine (T3)
Energy metabolism
Protein catabolism
Lipid catabolism
Osmoregulation
Sparus aurata
description Thyroid hormones, in particular 3,5,3′-triiodothyronine or T3, are involved in multiple physiological processes in mammals such as protein, fat and carbohydrate metabolism. However, the metabolic actions of T3 in fish are still not fully elucidated. We therefore tested the effects of T3 on Sparus aurata energy metabolism and osmoregulatory system, a hyperthyroid-induced model that was chosen. Fish were implanted with coconut oil depots (containing 0, 2.5, 5.0 and 10.0 μg T3/g body weight) and sampled at day 3 and 6 post-implantation. Plasma levels of free T3 as well as glucose, lactate and triglyceride values increased with increasing doses of T3 at days 3 and 6 post-implantation. Changes in plasma and organ metabolite levels (glucose, glycogen, triglycerides, lactate and total α amino acid) and enzyme activities related to carbohydrate, lactate, amino acid and lipid pathways were detected in organs involved in metabolism (liver) and osmoregulation (gills and kidney). Our data implicate that the liver uses amino acids as an energy source in response to the T3 treatment, increasing protein catabolism and gluconeogenic pathways. The gills, the most important extruder of ammonia, are fuelled not only by amino acids, but also by lactate. The kidney differs significantly in its substrate preference from the gills, as it obtained metabolic energy from lactate but also from lipid oxidation processes. We conclude that in S. aurata lipid catabolism and protein turnover are increased as a consequence of experimentally induced hyperthyroidism, with secondary osmoregulatory effects.
publishDate 2016
dc.date.none.fl_str_mv 2016
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/326232
url http://hdl.handle.net/10261/326232
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Málaga
https://doi.org/10.1016/j.cbpa.2015.09.014
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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