Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease
[EN]Childhood obesity has reached epidemic levels, representing one of the most serious public health concerns associated with metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). There is limited clinical experience concerning pediatric NAFLD patients, and thus the therapeutic options...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2019 |
| País: | España |
| Recursos: | Universidad de León |
| Repositório: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/17854 |
| Acesso em linha: | https://journals.biologists.com/dmm/article/12/5/dmm039206/3379/Beneficial-effects-of-exercise-on-gut-microbiota https://hdl.handle.net/10612/17854 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Fisiología Childhood obesity Fecal metabolome Gut-liver axis Intestinal microbiota Metabolic syndrome 32 Ciencias Médicas 3109.09 Fisiología |
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Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver diseaseCarbajo Pescador, SaraPorras Sanabria, DavidGarcía Mediavilla, María VictoriaMartínez Flórez, SusanaJuárez Fernández, MaríaCuevas González, María JoséMauriz Gutiérrez, José LuisGonzález Gallego, JavierNistal González, Maria EstherSánchez Campos, SoniaFisiologíaChildhood obesityFecal metabolomeGut-liver axisIntestinal microbiotaMetabolic syndrome32 Ciencias Médicas3109.09 Fisiología[EN]Childhood obesity has reached epidemic levels, representing one of the most serious public health concerns associated with metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). There is limited clinical experience concerning pediatric NAFLD patients, and thus the therapeutic options are scarce. The aim of this study was to evaluate the benefits of exercise on gut microbiota composition and functionality balance, and consequent effects on early obesity and NAFLD onset in an in vivo model. Juvenile (21-day-old) male Wistar rats fed a control diet or a high-fat diet (HFD) were subjected to a combined aerobic and resistance training protocol. Fecal microbiota was sequenced by an Illumina MiSeq system, and parameters related to metabolic syndrome, fecal metabolome, intestinal barrier integrity, bile acid metabolism and transport, and alteration of the gut-liver axis were measured. Exercise decreased HFD-induced body weight gain, metabolic syndrome and hepatic steatosis, as a result of its lipid metabolism modulatory capacity. Gut microbiota composition and functionality were substantially modified as a consequence of diet, age and exercise intervention. In addition, the training protocol increased Parabacteroides, Bacteroides and Flavobacterium genera, correlating with a beneficial metabolomic profile, whereas Blautia, Dysgonomonas and Porphyromonas showed an opposite pattern. Exercise effectively counteracted HFD-induced microbial imbalance, leading to intestinal barrier preservation, which, in turn, prevented deregulation of the gut-liver axis and improved bile acid homeostasis, determining the clinical outcomes of NAFLD. In conclusion, we provide scientific evidence highlighting the benefits of gut microbiota composition and functionality modulation by physical exercise protocols in the management of early obesity and NAFLD development.SIThis work was supported by grants from Ministerio de Economıa y Competitividad ́ (BFU2017-87960-R), Junta de Castilla y León and the European Regional Development Fund (FEDER) (LE063U16 and GRS1888/A/18). D.P. and S.C.-P. were supported by a fellowship from Junta de Castilla y León, co-financed by the European Social Fund. E.N. was supported by Fundación de Investigación Sanitaria of León. M.V.G.-M. was supported by contracts from the CIBERehd, which is funded by Instituto de Salud Carlos III.The Company of BiologistsFisiologiaFacultad de Veterinaria2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.biologists.com/dmm/article/12/5/dmm039206/3379/Beneficial-effects-of-exercise-on-gut-microbiotahttps://hdl.handle.net/10612/17854reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/ BFU2017-87960-Rhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/178542026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| title |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| spellingShingle |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease Carbajo Pescador, Sara Fisiología Childhood obesity Fecal metabolome Gut-liver axis Intestinal microbiota Metabolic syndrome 32 Ciencias Médicas 3109.09 Fisiología |
| title_short |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| title_full |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| title_fullStr |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| title_full_unstemmed |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| title_sort |
Beneficial effects of exercise on gut microbiota functionality and barrier integrity, and gut-liver axis crosstalk in an "in vivo" model of early obesity and non-alcoholic fatty liver disease |
| dc.creator.none.fl_str_mv |
Carbajo Pescador, Sara Porras Sanabria, David García Mediavilla, María Victoria Martínez Flórez, Susana Juárez Fernández, María Cuevas González, María José Mauriz Gutiérrez, José Luis González Gallego, Javier Nistal González, Maria Esther Sánchez Campos, Sonia |
| author |
Carbajo Pescador, Sara |
| author_facet |
Carbajo Pescador, Sara Porras Sanabria, David García Mediavilla, María Victoria Martínez Flórez, Susana Juárez Fernández, María Cuevas González, María José Mauriz Gutiérrez, José Luis González Gallego, Javier Nistal González, Maria Esther Sánchez Campos, Sonia |
| author_role |
author |
| author2 |
Porras Sanabria, David García Mediavilla, María Victoria Martínez Flórez, Susana Juárez Fernández, María Cuevas González, María José Mauriz Gutiérrez, José Luis González Gallego, Javier Nistal González, Maria Esther Sánchez Campos, Sonia |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Fisiologia Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Fisiología Childhood obesity Fecal metabolome Gut-liver axis Intestinal microbiota Metabolic syndrome 32 Ciencias Médicas 3109.09 Fisiología |
| topic |
Fisiología Childhood obesity Fecal metabolome Gut-liver axis Intestinal microbiota Metabolic syndrome 32 Ciencias Médicas 3109.09 Fisiología |
| description |
[EN]Childhood obesity has reached epidemic levels, representing one of the most serious public health concerns associated with metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). There is limited clinical experience concerning pediatric NAFLD patients, and thus the therapeutic options are scarce. The aim of this study was to evaluate the benefits of exercise on gut microbiota composition and functionality balance, and consequent effects on early obesity and NAFLD onset in an in vivo model. Juvenile (21-day-old) male Wistar rats fed a control diet or a high-fat diet (HFD) were subjected to a combined aerobic and resistance training protocol. Fecal microbiota was sequenced by an Illumina MiSeq system, and parameters related to metabolic syndrome, fecal metabolome, intestinal barrier integrity, bile acid metabolism and transport, and alteration of the gut-liver axis were measured. Exercise decreased HFD-induced body weight gain, metabolic syndrome and hepatic steatosis, as a result of its lipid metabolism modulatory capacity. Gut microbiota composition and functionality were substantially modified as a consequence of diet, age and exercise intervention. In addition, the training protocol increased Parabacteroides, Bacteroides and Flavobacterium genera, correlating with a beneficial metabolomic profile, whereas Blautia, Dysgonomonas and Porphyromonas showed an opposite pattern. Exercise effectively counteracted HFD-induced microbial imbalance, leading to intestinal barrier preservation, which, in turn, prevented deregulation of the gut-liver axis and improved bile acid homeostasis, determining the clinical outcomes of NAFLD. In conclusion, we provide scientific evidence highlighting the benefits of gut microbiota composition and functionality modulation by physical exercise protocols in the management of early obesity and NAFLD development. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://journals.biologists.com/dmm/article/12/5/dmm039206/3379/Beneficial-effects-of-exercise-on-gut-microbiota https://hdl.handle.net/10612/17854 |
| url |
https://journals.biologists.com/dmm/article/12/5/dmm039206/3379/Beneficial-effects-of-exercise-on-gut-microbiota https://hdl.handle.net/10612/17854 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
info:eu-repo/grantAgreement/AEI/ Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/ BFU2017-87960-R |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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The Company of Biologists |
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The Company of Biologists |
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reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad de León |
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Universidad de León |
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