Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus

[EN] Antimicrobial peptides (AMPs) are a potent arm of the innate immune system that can directly kill pathogens and induce immunomodulation. In the marine aquaculture, European sea bass (Dicentrarchus labrax L.) is one of the most prosperous species but is highly susceptible to nodavirus (NNV), whi...

ver descrição completa

Detalhes bibliográficos
Autores: Cervera, Laura, González-Fernández, Carmen, Cano, Daniela, Esteban, María Ángeles, Mercado, Luis, Chaves-Pozo, Elena, Cuesta, Alberto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/286891
Acesso em linha:http://hdl.handle.net/10261/286891
https://api.elsevier.com/content/abstract/scopus_id/85145291722
Access Level:acceso abierto
Palavra-chave:Antimicrobial peptides
Dicentracin
Dicentrarchus labrax
Hepcidin
Immunostimulation
NK-lysin
Nodavirus
id ES_e39a8cde41ea02346df79314d88ebecf
oai_identifier_str oai:digital.csic.es:10261/286891
network_acronym_str ES
network_name_str España
repository_id_str
spelling Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirusCervera, LauraGonzález-Fernández, CarmenCano, DanielaEsteban, María ÁngelesMercado, LuisChaves-Pozo, ElenaCuesta, AlbertoAntimicrobial peptidesDicentracinDicentrarchus labraxHepcidinImmunostimulationNK-lysinNodavirus[EN] Antimicrobial peptides (AMPs) are a potent arm of the innate immune system that can directly kill pathogens and induce immunomodulation. In the marine aquaculture, European sea bass (Dicentrarchus labrax L.) is one of the most prosperous species but is highly susceptible to nodavirus (NNV), which produces high rates of mortality in larvae and juvenile stages. Thus, we aimed to evaluate whether AMPs exert immunomodulatory and/or NNV-preventive actions in sea bass. To do this, plasmids encoding the sea bass AMPs dicentracin (pDIC), beta-defensin (pDB1), hepcidin (pHAMP2) or NK-lysin (pNKL) were generated and intramuscularly injected into sea bass juveniles to evaluate their immunomodulatory and anti-NNV roles. Sea bass muscle transcribes the AMPs and produces an increase in their circulating levels, along with an increase of the antibacterial activity. Immune-related gene analysis revealed a great activation of the inflammatory response and the recruitment of neutrophilic granulocytes at the site of injection. However, AMP-encoding plasmids, namely pHAMP2, negatively affected to NNV disease by increasing fish mortality. In conclusion, plasmids encoding AMPs show immunostimulatory effects on European sea bass but do not improve the resistance to NNV.This work was funded by Ministerio de Ciencia e Innovación-Agencia Estatal de Investigación (MCIN/AEI/10.13039/501100011033, grant PID2019-105522 GB-I00 to A.C. and PRE2020-093771 to L.C.), Ministerio de Economía y Competitividad and FEDER (grant RTI2018-096625-B-C33 to E.C.P.) and Fundación Séneca, Grupo de Excelencia de la Región de Murcia (19883/GERM/15).Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Fundación SénecaChaves-Pozo, Elena [0000-0003-4030-1273]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/286891https://api.elsevier.com/content/abstract/scopus_id/85145291722reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105522GB-I00Fish & shellfish immunologyhttps://doi.org/10.1016/j.fsi.2022.108507Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2868912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
title Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
spellingShingle Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
Cervera, Laura
Antimicrobial peptides
Dicentracin
Dicentrarchus labrax
Hepcidin
Immunostimulation
NK-lysin
Nodavirus
title_short Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
title_full Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
title_fullStr Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
title_full_unstemmed Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
title_sort Immunity elicited by AMP-encoding plasmids fails to increase the protection of European sea bass against nodavirus
dc.creator.none.fl_str_mv Cervera, Laura
González-Fernández, Carmen
Cano, Daniela
Esteban, María Ángeles
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author Cervera, Laura
author_facet Cervera, Laura
González-Fernández, Carmen
Cano, Daniela
Esteban, María Ángeles
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author_role author
author2 González-Fernández, Carmen
Cano, Daniela
Esteban, María Ángeles
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Fundación Séneca
Chaves-Pozo, Elena [0000-0003-4030-1273]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antimicrobial peptides
Dicentracin
Dicentrarchus labrax
Hepcidin
Immunostimulation
NK-lysin
Nodavirus
topic Antimicrobial peptides
Dicentracin
Dicentrarchus labrax
Hepcidin
Immunostimulation
NK-lysin
Nodavirus
description [EN] Antimicrobial peptides (AMPs) are a potent arm of the innate immune system that can directly kill pathogens and induce immunomodulation. In the marine aquaculture, European sea bass (Dicentrarchus labrax L.) is one of the most prosperous species but is highly susceptible to nodavirus (NNV), which produces high rates of mortality in larvae and juvenile stages. Thus, we aimed to evaluate whether AMPs exert immunomodulatory and/or NNV-preventive actions in sea bass. To do this, plasmids encoding the sea bass AMPs dicentracin (pDIC), beta-defensin (pDB1), hepcidin (pHAMP2) or NK-lysin (pNKL) were generated and intramuscularly injected into sea bass juveniles to evaluate their immunomodulatory and anti-NNV roles. Sea bass muscle transcribes the AMPs and produces an increase in their circulating levels, along with an increase of the antibacterial activity. Immune-related gene analysis revealed a great activation of the inflammatory response and the recruitment of neutrophilic granulocytes at the site of injection. However, AMP-encoding plasmids, namely pHAMP2, negatively affected to NNV disease by increasing fish mortality. In conclusion, plasmids encoding AMPs show immunostimulatory effects on European sea bass but do not improve the resistance to NNV.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/286891
https://api.elsevier.com/content/abstract/scopus_id/85145291722
url http://hdl.handle.net/10261/286891
https://api.elsevier.com/content/abstract/scopus_id/85145291722
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105522GB-I00
Fish & shellfish immunology
https://doi.org/10.1016/j.fsi.2022.108507
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422516333707264
score 15,812455

Registos relacionados