Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of m...

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Authors: Benedé Pérez, Sara, Gradillas, Ana, Villalba Díaz, María Teresa, Batanero Cremades, Eva
Format: article
Publication Date:2019
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/12642
Online Access:https://hdl.handle.net/20.500.14352/12642
Access Level:Open access
Keyword:Allium porrum
antioxidant activity
phenolic compounds
mast cell
epithelium
Biología molecular (Química)
Bioquímica (Química)
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12642
network_acronym_str ES
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repository_id_str
spelling Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell FunctionBenedé Pérez, SaraGradillas, AnaVillalba Díaz, María TeresaBatanero Cremades, EvaAllium porrumantioxidant activityphenolic compoundsmast cellepitheliumBiología molecular (Química)Bioquímica (Química)Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy.MDPIUniversidad Complutense de Madrid20192019-06-0820192019-06-08journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12642reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/126422026-06-02T12:44:21Z
dc.title.none.fl_str_mv Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
title Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
spellingShingle Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
Benedé Pérez, Sara
Allium porrum
antioxidant activity
phenolic compounds
mast cell
epithelium
Biología molecular (Química)
Bioquímica (Química)
title_short Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
title_full Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
title_fullStr Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
title_full_unstemmed Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
title_sort Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function
dc.creator.none.fl_str_mv Benedé Pérez, Sara
Gradillas, Ana
Villalba Díaz, María Teresa
Batanero Cremades, Eva
author Benedé Pérez, Sara
author_facet Benedé Pérez, Sara
Gradillas, Ana
Villalba Díaz, María Teresa
Batanero Cremades, Eva
author_role author
author2 Gradillas, Ana
Villalba Díaz, María Teresa
Batanero Cremades, Eva
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Allium porrum
antioxidant activity
phenolic compounds
mast cell
epithelium
Biología molecular (Química)
Bioquímica (Química)
topic Allium porrum
antioxidant activity
phenolic compounds
mast cell
epithelium
Biología molecular (Química)
Bioquímica (Química)
description Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-06-08
2019
2019-06-08
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12642
url https://hdl.handle.net/20.500.14352/12642
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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