Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture o...
| Authors: | , , |
|---|---|
| Format: | article |
| Status: | Published version |
| Publication Date: | 2013 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/151262 |
| Online Access: | https://hdl.handle.net/2445/151262 |
| Access Level: | Open access |
| Keyword: | Àcids nucleics G-estructures Oligonucleòtids Nucleic acids G-structures Oligonucleotides |
| Summary: | The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles. |
|---|