Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus

Brain aquaporins facilitate the movement of water between the four water compartments: blood, cerebrospinal fluid, interstitial fluid, and intracellular fluid. This work analyzes the expression of the four most abundant aquaporins (AQPs) (AQP1, AQP4, AQP9, and AQP11) in the brains of mice and discus...

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Autores: Trillo Contreras, José Luis, Ramírez Lorca, Reposo, Villadiego Luque, Francisco Javier, Echevarría Irusta, Miriam
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/138038
Acesso em linha:https://hdl.handle.net/11441/138038
https://doi.org/10.3390/biom12040530
Access Level:acceso abierto
Palavra-chave:Aquaporins
Cerebrospinal fluid
Choroid plexus
Ependyma
Single-cell RNA
Sequencing
Hydrocephalus
Aging
Hypoxia
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spelling Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalusTrillo Contreras, José LuisRamírez Lorca, ReposoVilladiego Luque, Francisco JavierEchevarría Irusta, MiriamAquaporinsCerebrospinal fluidChoroid plexusEpendymaSingle-cell RNASequencingHydrocephalusAgingHypoxiaBrain aquaporins facilitate the movement of water between the four water compartments: blood, cerebrospinal fluid, interstitial fluid, and intracellular fluid. This work analyzes the expression of the four most abundant aquaporins (AQPs) (AQP1, AQP4, AQP9, and AQP11) in the brains of mice and discuss their contribution to hydrocephalus. We analyzed available data from single-cell RNA sequencing of the central nervous system of mice to describe the expression of aquaporins and compare their distribution with that based on qPCR, western blot, and immunohistochemistry assays. Expression of AQP1 in the apical cell membrane of choroid plexus epithelial cells and of AQP4 in ependymal cells, glia limitans, and astrocyte processes in the pericapillary end foot is consistent with the involvement of both proteins in cerebrospinal fluid homeostasis. The expression of both aquaporins compensates for experimentally induced hydrocephalus in the animals. Recent data demonstrate that hypoxia in aged animals alters AQP4 expression in the choroidal plexus and cortex, increasing the ventricle size and intraventricular pressure. Cerebral distensibility is reduced in parallel with a reduction in cerebrospinal fluid drainage and cognitive deterioration. We propose that aged mice chronically exposed to hypoxia represent an excellent experimental model for studying the pathophysiological characteristics of idiopathic normal pressure hydrocephalus and roles for AQPs in such disease.MDPIFisiología Médica y Biofísica2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/138038https://doi.org/10.3390/biom12040530reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBiomolecules, 12 (4), 1-18.https://www.mdpi.com/2218-273X/12/4/530info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1380382026-06-17T12:51:07Z
dc.title.none.fl_str_mv Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
title Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
spellingShingle Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
Trillo Contreras, José Luis
Aquaporins
Cerebrospinal fluid
Choroid plexus
Ependyma
Single-cell RNA
Sequencing
Hydrocephalus
Aging
Hypoxia
title_short Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
title_full Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
title_fullStr Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
title_full_unstemmed Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
title_sort Cellular distribution of brain aquaporins and their contribution to cerebrospinal fluid homeostasis and hydrocephalus
dc.creator.none.fl_str_mv Trillo Contreras, José Luis
Ramírez Lorca, Reposo
Villadiego Luque, Francisco Javier
Echevarría Irusta, Miriam
author Trillo Contreras, José Luis
author_facet Trillo Contreras, José Luis
Ramírez Lorca, Reposo
Villadiego Luque, Francisco Javier
Echevarría Irusta, Miriam
author_role author
author2 Ramírez Lorca, Reposo
Villadiego Luque, Francisco Javier
Echevarría Irusta, Miriam
author2_role author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
dc.subject.none.fl_str_mv Aquaporins
Cerebrospinal fluid
Choroid plexus
Ependyma
Single-cell RNA
Sequencing
Hydrocephalus
Aging
Hypoxia
topic Aquaporins
Cerebrospinal fluid
Choroid plexus
Ependyma
Single-cell RNA
Sequencing
Hydrocephalus
Aging
Hypoxia
description Brain aquaporins facilitate the movement of water between the four water compartments: blood, cerebrospinal fluid, interstitial fluid, and intracellular fluid. This work analyzes the expression of the four most abundant aquaporins (AQPs) (AQP1, AQP4, AQP9, and AQP11) in the brains of mice and discuss their contribution to hydrocephalus. We analyzed available data from single-cell RNA sequencing of the central nervous system of mice to describe the expression of aquaporins and compare their distribution with that based on qPCR, western blot, and immunohistochemistry assays. Expression of AQP1 in the apical cell membrane of choroid plexus epithelial cells and of AQP4 in ependymal cells, glia limitans, and astrocyte processes in the pericapillary end foot is consistent with the involvement of both proteins in cerebrospinal fluid homeostasis. The expression of both aquaporins compensates for experimentally induced hydrocephalus in the animals. Recent data demonstrate that hypoxia in aged animals alters AQP4 expression in the choroidal plexus and cortex, increasing the ventricle size and intraventricular pressure. Cerebral distensibility is reduced in parallel with a reduction in cerebrospinal fluid drainage and cognitive deterioration. We propose that aged mice chronically exposed to hypoxia represent an excellent experimental model for studying the pathophysiological characteristics of idiopathic normal pressure hydrocephalus and roles for AQPs in such disease.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/138038
https://doi.org/10.3390/biom12040530
url https://hdl.handle.net/11441/138038
https://doi.org/10.3390/biom12040530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biomolecules, 12 (4), 1-18.
https://www.mdpi.com/2218-273X/12/4/530
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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