Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period

Background: Surveillance studies are crucial for updating trends in Aspergillus species and antifungal susceptibility information. Objectives: Determine the Aspergillus species distribution and azole resistance prevalence during this 3-year prospective surveillance study in a Spanish hospital. Mater...

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Autores: Lucio, Jose, Alcazar-Fuoli, Laura, Gil, Horacio, Cano-Pascual, Samuel, Hernandez-Egido, Sara, Cuetara, Maria Soledad, Mellado, Emilia
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/19922
Acesso em linha:http://hdl.handle.net/20.500.12105/19922
Access Level:acceso abierto
Palavra-chave:Aspergillosis
Aspergillus nidulans
Humans
Azoles
Antifungal Agents
Prevalence
Prospective Studies
Drug Resistance, Fungal
Aspergillus fumigatus
Hospitals
Fungal Proteins
Microbial Sensitivity Tests
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spelling Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study periodLucio, JoseAlcazar-Fuoli, LauraGil, HoracioCano-Pascual, SamuelHernandez-Egido, SaraCuetara, Maria SoledadMellado, EmiliaAspergillosisAspergillus nidulansHumansAzolesAntifungal AgentsPrevalenceProspective StudiesDrug Resistance, FungalAspergillus fumigatusHospitalsFungal ProteinsMicrobial Sensitivity TestsBackground: Surveillance studies are crucial for updating trends in Aspergillus species and antifungal susceptibility information. Objectives: Determine the Aspergillus species distribution and azole resistance prevalence during this 3-year prospective surveillance study in a Spanish hospital. Materials and methods: Three hundred thirty-five Aspergillus spp. clinical and environmental isolates were collected during a 3-year study. All isolates were screened for azole resistance using an agar-based screening method and resistance was confirmed by EUCAST antifungal susceptibility testing. The azole resistance mechanism was confirmed by sequencing the cyp51A gene and its promoter. All Aspergillus fumigatus strains were genotyped using TRESPERG analysis. Results: Aspergillus fumigatus was the predominant species recovered with a total of 174 strains (51.94%). The rest of Aspergillus spp. were less frequent: Aspergillus niger (14.93%), Aspergillus terreus (9.55%), Aspergillus flavus (8.36%), Aspergillus nidulans (5.37%) and Aspergillus lentulus (3.28%), among other Aspergillus species (6.57%). TRESPERG analysis showed 99 different genotypes, with 72.73% of the strains being represented as a single genotype. Some genotypes were common among clinical and environmental A. fumigatus azole-susceptible strains, even when isolated months apart. We describe the occurrence of two azole-resistant A. fumigatus strains, one clinical and another environmental, that were genotypically different and did not share genotypes with any of the azole-susceptible strains. Conclusions: Aspergillus fumigatus strains showed a very diverse population although several genotypes were shared among clinical and environmental strains. The isolation of azole-resistant strains from both settings suggest that an efficient analysis of clinical and environmental sources must be done to detect azole resistance in A. fumigatus.WileyInstituto de Salud Carlos IIICentro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)20242024-07-0320242024-04-0120242024-04-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12105/19922reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/199222026-06-12T12:43:37Z
dc.title.none.fl_str_mv Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
title Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
spellingShingle Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
Lucio, Jose
Aspergillosis
Aspergillus nidulans
Humans
Azoles
Antifungal Agents
Prevalence
Prospective Studies
Drug Resistance, Fungal
Aspergillus fumigatus
Hospitals
Fungal Proteins
Microbial Sensitivity Tests
title_short Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
title_full Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
title_fullStr Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
title_full_unstemmed Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
title_sort Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
dc.creator.none.fl_str_mv Lucio, Jose
Alcazar-Fuoli, Laura
Gil, Horacio
Cano-Pascual, Samuel
Hernandez-Egido, Sara
Cuetara, Maria Soledad
Mellado, Emilia
author Lucio, Jose
author_facet Lucio, Jose
Alcazar-Fuoli, Laura
Gil, Horacio
Cano-Pascual, Samuel
Hernandez-Egido, Sara
Cuetara, Maria Soledad
Mellado, Emilia
author_role author
author2 Alcazar-Fuoli, Laura
Gil, Horacio
Cano-Pascual, Samuel
Hernandez-Egido, Sara
Cuetara, Maria Soledad
Mellado, Emilia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
dc.subject.none.fl_str_mv Aspergillosis
Aspergillus nidulans
Humans
Azoles
Antifungal Agents
Prevalence
Prospective Studies
Drug Resistance, Fungal
Aspergillus fumigatus
Hospitals
Fungal Proteins
Microbial Sensitivity Tests
topic Aspergillosis
Aspergillus nidulans
Humans
Azoles
Antifungal Agents
Prevalence
Prospective Studies
Drug Resistance, Fungal
Aspergillus fumigatus
Hospitals
Fungal Proteins
Microbial Sensitivity Tests
description Background: Surveillance studies are crucial for updating trends in Aspergillus species and antifungal susceptibility information. Objectives: Determine the Aspergillus species distribution and azole resistance prevalence during this 3-year prospective surveillance study in a Spanish hospital. Materials and methods: Three hundred thirty-five Aspergillus spp. clinical and environmental isolates were collected during a 3-year study. All isolates were screened for azole resistance using an agar-based screening method and resistance was confirmed by EUCAST antifungal susceptibility testing. The azole resistance mechanism was confirmed by sequencing the cyp51A gene and its promoter. All Aspergillus fumigatus strains were genotyped using TRESPERG analysis. Results: Aspergillus fumigatus was the predominant species recovered with a total of 174 strains (51.94%). The rest of Aspergillus spp. were less frequent: Aspergillus niger (14.93%), Aspergillus terreus (9.55%), Aspergillus flavus (8.36%), Aspergillus nidulans (5.37%) and Aspergillus lentulus (3.28%), among other Aspergillus species (6.57%). TRESPERG analysis showed 99 different genotypes, with 72.73% of the strains being represented as a single genotype. Some genotypes were common among clinical and environmental A. fumigatus azole-susceptible strains, even when isolated months apart. We describe the occurrence of two azole-resistant A. fumigatus strains, one clinical and another environmental, that were genotypically different and did not share genotypes with any of the azole-susceptible strains. Conclusions: Aspergillus fumigatus strains showed a very diverse population although several genotypes were shared among clinical and environmental strains. The isolation of azole-resistant strains from both settings suggest that an efficient analysis of clinical and environmental sources must be done to detect azole resistance in A. fumigatus.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-07-03
2024
2024-04-01
2024
2024-04-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/19922
url http://hdl.handle.net/20.500.12105/19922
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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