Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula

The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in hum...

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Autores: Köster, Pamela C., Figueiredo, Ana M., Maloney, Jenny G., Dashti, Alejandro, Bailo, Begoña, Torres, Rita T., Fonseca, Carlos, Mysterud, Atle, Habela, Miguel Á., Rivero Juarez, Antonio, Vicente, Joaquín, Serrano, Emmanuel, Arnal, Maria C., de Luco, Daniel Fernández, Armenteros, José A., Balseiro, Ana, Cardona, Guillermo A., Carvalho, João, Hipólito, Dário, Fernandes, Joana, Palmeira, Josman D., Calero Bernal, Rafael, González Barrio, David, Santin, Monica, Carmena, David
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/122321
Acceso en línea:https://hdl.handle.net/20.500.14352/122321
Access Level:acceso abierto
Palabra clave:579.62
Epidemiology
NGS
Subtype diversity wildlife
Zoonoses
Spain
Portugal
Microbiología (Veterinaria)
3109.05 Microbiología
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spelling Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian PeninsulaKöster, Pamela C.Figueiredo, Ana M.Maloney, Jenny G.Dashti, AlejandroBailo, BegoñaTorres, Rita T.Fonseca, CarlosMysterud, AtleHabela, Miguel Á.Rivero Juarez, AntonioVicente, JoaquínSerrano, EmmanuelArnal, Maria C.de Luco, Daniel FernándezArmenteros, José A.Balseiro, AnaCardona, Guillermo A.Carvalho, JoãoHipólito, DárioFernandes, JoanaPalmeira, Josman D.Calero Bernal, RafaelGonzález Barrio, DavidSantin, MonicaCarmena, David579.62EpidemiologyNGSSubtype diversity wildlifeZoonosesSpainPortugalMicrobiología (Veterinaria)3109.05 MicrobiologíaThe ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15.3% (70/459; 95% CI 12.1-18.9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34.3%, 34/99; 95% CI 25.1-44.6) than in Spain (10.0%, 36/360; 95% CI 7.1-13.6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14.3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significanceBioMed CentralUniversidad Complutense de Madrid20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/122321reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1223212026-06-02T12:44:21Z
dc.title.none.fl_str_mv Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
title Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
spellingShingle Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
Köster, Pamela C.
579.62
Epidemiology
NGS
Subtype diversity wildlife
Zoonoses
Spain
Portugal
Microbiología (Veterinaria)
3109.05 Microbiología
title_short Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
title_full Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
title_fullStr Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
title_full_unstemmed Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
title_sort Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula
dc.creator.none.fl_str_mv Köster, Pamela C.
Figueiredo, Ana M.
Maloney, Jenny G.
Dashti, Alejandro
Bailo, Begoña
Torres, Rita T.
Fonseca, Carlos
Mysterud, Atle
Habela, Miguel Á.
Rivero Juarez, Antonio
Vicente, Joaquín
Serrano, Emmanuel
Arnal, Maria C.
de Luco, Daniel Fernández
Armenteros, José A.
Balseiro, Ana
Cardona, Guillermo A.
Carvalho, João
Hipólito, Dário
Fernandes, Joana
Palmeira, Josman D.
Calero Bernal, Rafael
González Barrio, David
Santin, Monica
Carmena, David
author Köster, Pamela C.
author_facet Köster, Pamela C.
Figueiredo, Ana M.
Maloney, Jenny G.
Dashti, Alejandro
Bailo, Begoña
Torres, Rita T.
Fonseca, Carlos
Mysterud, Atle
Habela, Miguel Á.
Rivero Juarez, Antonio
Vicente, Joaquín
Serrano, Emmanuel
Arnal, Maria C.
de Luco, Daniel Fernández
Armenteros, José A.
Balseiro, Ana
Cardona, Guillermo A.
Carvalho, João
Hipólito, Dário
Fernandes, Joana
Palmeira, Josman D.
Calero Bernal, Rafael
González Barrio, David
Santin, Monica
Carmena, David
author_role author
author2 Figueiredo, Ana M.
Maloney, Jenny G.
Dashti, Alejandro
Bailo, Begoña
Torres, Rita T.
Fonseca, Carlos
Mysterud, Atle
Habela, Miguel Á.
Rivero Juarez, Antonio
Vicente, Joaquín
Serrano, Emmanuel
Arnal, Maria C.
de Luco, Daniel Fernández
Armenteros, José A.
Balseiro, Ana
Cardona, Guillermo A.
Carvalho, João
Hipólito, Dário
Fernandes, Joana
Palmeira, Josman D.
Calero Bernal, Rafael
González Barrio, David
Santin, Monica
Carmena, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.62
Epidemiology
NGS
Subtype diversity wildlife
Zoonoses
Spain
Portugal
Microbiología (Veterinaria)
3109.05 Microbiología
topic 579.62
Epidemiology
NGS
Subtype diversity wildlife
Zoonoses
Spain
Portugal
Microbiología (Veterinaria)
3109.05 Microbiología
description The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15.3% (70/459; 95% CI 12.1-18.9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34.3%, 34/99; 95% CI 25.1-44.6) than in Spain (10.0%, 36/360; 95% CI 7.1-13.6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14.3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significance
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/122321
url https://hdl.handle.net/20.500.14352/122321
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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