Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis

Jenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa)....

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Autores: Yáñez-Ortiz, Iván, Catalán, Jaime, Mateo Otero, Yentel, Dordas-Perpinyà, Marta, Gacem, Sabrina, Yeste, Natalia, Bassols, Anna, Yeste Oliveras, Marc, Miró, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/19843
Acceso en línea:http://hdl.handle.net/10256/19843
Access Level:acceso abierto
Palabra clave:Espermatozoides
Spermatozoa
Ases -- Espermatozoides
Donkeys -- Spermatozoa
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spelling Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosisYáñez-Ortiz, IvánCatalán, JaimeMateo Otero, YentelDordas-Perpinyà, MartaGacem, SabrinaYeste, NataliaBassols, AnnaYeste Oliveras, MarcMiró, JordiEspermatozoidesSpermatozoaAses -- EspermatozoidesDonkeys -- SpermatozoaJenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa). While a reduced percentage of spermatozoa are phagocytosed by PMN, most are found to be attached to neutrophil extracellular traps (NETs). This selection process together with sperm metabolism produces a large amount of reactive oxygen species (ROS) that influence the reproductive success. The present study aimed to determine the extracellular ROS production in both sperm and PMN. With this purpose, (1) donkey sperm were exposed to reductive and oxidative stresses, through adding different concentrations of reduced glutathione (GSH) and hydrogen peroxide (H2O2), respectively; and (2) PMN were subjected to NETosis in the presence of the whole semen, sperm, seminal plasma (SP) or other activators such as formyl-methionyl-leucyl-phenylalanine (FMLP). Extracellular ROS production (measured as H2O2 levels) was determined with the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Donkey sperm showed more resilience to oxidative stress than to the reductive one, and GSH treatments led to greater H2O2 extracellular production. Moreover, not only did SP appear to be the main inducer of NETosis in PMN, but it was also able to maintain the extracellular H2O2 levels produced by sperm and NETosisI.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019). J.C. was funded by the National Agency for Research and Development (ANID), Ministry of Education, Chile (Scheme: Becas Chile Doctorado en el Extranjero, PFCHA; Grant: 2017/72180128). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grant: AGL2017-88329-R) and the Regional Government of Catalonia (2017-SGR-1229MDPI (Multidisciplinary Digital Publishing Institute)Agencia Estatal de Investigación2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/19843http://hdl.handle.net/10256/19843Antioxidants, 2021, vol. 10, núm. 9, p. 1367Articles publicats (D-B)Yáñez-Ortiz, Iván Catalán, Jaime Mateo Otero, Yentel Dordas-Perpinyà, Marta Gacem, Sabrina Yeste, Natalia Bassols, Anna Yeste Oliveras, Marc Miró, Jordi 2021 Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis Antioxidants 10 9 1367reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/antiox10091367info:eu-repo/semantics/altIdentifier/eissn/2076-3921info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-RAttribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/198432026-05-29T05:05:01Z
dc.title.none.fl_str_mv Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
title Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
spellingShingle Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
Yáñez-Ortiz, Iván
Espermatozoides
Spermatozoa
Ases -- Espermatozoides
Donkeys -- Spermatozoa
title_short Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
title_full Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
title_fullStr Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
title_full_unstemmed Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
title_sort Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis
dc.creator.none.fl_str_mv Yáñez-Ortiz, Iván
Catalán, Jaime
Mateo Otero, Yentel
Dordas-Perpinyà, Marta
Gacem, Sabrina
Yeste, Natalia
Bassols, Anna
Yeste Oliveras, Marc
Miró, Jordi
author Yáñez-Ortiz, Iván
author_facet Yáñez-Ortiz, Iván
Catalán, Jaime
Mateo Otero, Yentel
Dordas-Perpinyà, Marta
Gacem, Sabrina
Yeste, Natalia
Bassols, Anna
Yeste Oliveras, Marc
Miró, Jordi
author_role author
author2 Catalán, Jaime
Mateo Otero, Yentel
Dordas-Perpinyà, Marta
Gacem, Sabrina
Yeste, Natalia
Bassols, Anna
Yeste Oliveras, Marc
Miró, Jordi
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Espermatozoides
Spermatozoa
Ases -- Espermatozoides
Donkeys -- Spermatozoa
topic Espermatozoides
Spermatozoa
Ases -- Espermatozoides
Donkeys -- Spermatozoa
description Jenny shows a large endometrial reaction after semen influx to the uterus with a large amount of polymorphonuclear neutrophils (PMN) migrating into the uterine lumen. PMN act as a sperm selection mechanism through phagocytosis and NETosis (DNA extrudes and, together with proteins, trap spermatozoa). While a reduced percentage of spermatozoa are phagocytosed by PMN, most are found to be attached to neutrophil extracellular traps (NETs). This selection process together with sperm metabolism produces a large amount of reactive oxygen species (ROS) that influence the reproductive success. The present study aimed to determine the extracellular ROS production in both sperm and PMN. With this purpose, (1) donkey sperm were exposed to reductive and oxidative stresses, through adding different concentrations of reduced glutathione (GSH) and hydrogen peroxide (H2O2), respectively; and (2) PMN were subjected to NETosis in the presence of the whole semen, sperm, seminal plasma (SP) or other activators such as formyl-methionyl-leucyl-phenylalanine (FMLP). Extracellular ROS production (measured as H2O2 levels) was determined with the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Donkey sperm showed more resilience to oxidative stress than to the reductive one, and GSH treatments led to greater H2O2 extracellular production. Moreover, not only did SP appear to be the main inducer of NETosis in PMN, but it was also able to maintain the extracellular H2O2 levels produced by sperm and NETosis
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/19843
http://hdl.handle.net/10256/19843
url http://hdl.handle.net/10256/19843
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox10091367
info:eu-repo/semantics/altIdentifier/eissn/2076-3921
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
dc.rights.none.fl_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv Antioxidants, 2021, vol. 10, núm. 9, p. 1367
Articles publicats (D-B)
Yáñez-Ortiz, Iván Catalán, Jaime Mateo Otero, Yentel Dordas-Perpinyà, Marta Gacem, Sabrina Yeste, Natalia Bassols, Anna Yeste Oliveras, Marc Miró, Jordi 2021 Extracellular reactive oxygen species (ROS) production in fresh donkey sperm exposed to reductive stress, oxidative stress and NETosis Antioxidants 10 9 1367
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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