Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.

Patients sensitized to Lipid transfer protein (LTP) were classified as anaphylaxis or sensitized depending on the symptoms elicited by LTP-containing food. CD34+-derived MCs from patients and controls were obtained, sensitized with pooled sera, and challenged with Pru p 3 (peach LTP). Degranulation,...

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Autores: Ollé, Laia, García García, Lucía, Ruano Zaragoza, Maria, Bartra Tomàs, Joan, González Navarro, E. Azucena, Pérez, Maria, Roca Ferrer, Jordi, Pasca, MI, Martín Andorrà, Margarita, Muñoz-Cano, Rosa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/218141
Acceso en línea:https://hdl.handle.net/2445/218141
Access Level:acceso abierto
Palabra clave:Al·lèrgia alimentària
Inflamació
Mastòcits
Anafilaxi
Food allergy
Inflammation
Mast cells
Anaphylaxis
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spelling Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.Ollé, LaiaGarcía García, LucíaRuano Zaragoza, MariaBartra Tomàs, JoanGonzález Navarro, E. AzucenaPérez, MariaRoca Ferrer, JordiPasca, MIMartín Andorrà, MargaritaMuñoz-Cano, RosaAl·lèrgia alimentàriaInflamacióMastòcitsAnafilaxiFood allergyInflammationMast cellsAnaphylaxisPatients sensitized to Lipid transfer protein (LTP) were classified as anaphylaxis or sensitized depending on the symptoms elicited by LTP-containing food. CD34+-derived MCs from patients and controls were obtained, sensitized with pooled sera, and challenged with Pru p 3 (peach LTP). Degranulation, PGD2, and cytokine/chemokine release were measured. The TFH13 population was examined by flow cytometry in the peripheral blood of all groups. In parallel, LAD2 cells were activated similarly to patients’ MCs. A distinguishable pattern of mast cell activation was found in anaphylaxis compared to sensitized patients. Robust degranulation, PGD2, and IL-8 and GM-CSF secretion were higher in anaphylaxis, whereas TFG-beta and CCL2 secretion increased in sensitized patients. Concomitantly, anaphylaxis patients had a larger TFH13 population. MC activation profile was dependent on the sera rather than the MC source. In agreement with that, LAD2 cells reproduce the same pattern as MCs from anaphylactic and sensitized patients. The distinct profile of mast cell activation allows for the discrimination between anaphylaxis and sensitized patients. Pooled sera may determine mast cell activation independently of mast cell origin. Besides, the presence of TFH13 cells in anaphylaxis patients points to an essential role of IgE affinity.Esmon Publicidad S.A.2025202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion27 p.application/pdfhttps://hdl.handle.net/2445/218141Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.18176/jiaci.0995Journal of Investigational Allergology and Clinical Immunology, 2024, vol. 35, num.3https://doi.org/10.18176/jiaci.0995(c) Esmon Publicidad S.A., 2024info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2181412026-05-29T05:05:01Z
dc.title.none.fl_str_mv Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
title Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
spellingShingle Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
Ollé, Laia
Al·lèrgia alimentària
Inflamació
Mastòcits
Anafilaxi
Food allergy
Inflammation
Mast cells
Anaphylaxis
title_short Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
title_full Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
title_fullStr Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
title_full_unstemmed Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
title_sort Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.
dc.creator.none.fl_str_mv Ollé, Laia
García García, Lucía
Ruano Zaragoza, Maria
Bartra Tomàs, Joan
González Navarro, E. Azucena
Pérez, Maria
Roca Ferrer, Jordi
Pasca, MI
Martín Andorrà, Margarita
Muñoz-Cano, Rosa
author Ollé, Laia
author_facet Ollé, Laia
García García, Lucía
Ruano Zaragoza, Maria
Bartra Tomàs, Joan
González Navarro, E. Azucena
Pérez, Maria
Roca Ferrer, Jordi
Pasca, MI
Martín Andorrà, Margarita
Muñoz-Cano, Rosa
author_role author
author2 García García, Lucía
Ruano Zaragoza, Maria
Bartra Tomàs, Joan
González Navarro, E. Azucena
Pérez, Maria
Roca Ferrer, Jordi
Pasca, MI
Martín Andorrà, Margarita
Muñoz-Cano, Rosa
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Al·lèrgia alimentària
Inflamació
Mastòcits
Anafilaxi
Food allergy
Inflammation
Mast cells
Anaphylaxis
topic Al·lèrgia alimentària
Inflamació
Mastòcits
Anafilaxi
Food allergy
Inflammation
Mast cells
Anaphylaxis
description Patients sensitized to Lipid transfer protein (LTP) were classified as anaphylaxis or sensitized depending on the symptoms elicited by LTP-containing food. CD34+-derived MCs from patients and controls were obtained, sensitized with pooled sera, and challenged with Pru p 3 (peach LTP). Degranulation, PGD2, and cytokine/chemokine release were measured. The TFH13 population was examined by flow cytometry in the peripheral blood of all groups. In parallel, LAD2 cells were activated similarly to patients’ MCs. A distinguishable pattern of mast cell activation was found in anaphylaxis compared to sensitized patients. Robust degranulation, PGD2, and IL-8 and GM-CSF secretion were higher in anaphylaxis, whereas TFG-beta and CCL2 secretion increased in sensitized patients. Concomitantly, anaphylaxis patients had a larger TFH13 population. MC activation profile was dependent on the sera rather than the MC source. In agreement with that, LAD2 cells reproduce the same pattern as MCs from anaphylactic and sensitized patients. The distinct profile of mast cell activation allows for the discrimination between anaphylaxis and sensitized patients. Pooled sera may determine mast cell activation independently of mast cell origin. Besides, the presence of TFH13 cells in anaphylaxis patients points to an essential role of IgE affinity.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218141
url https://hdl.handle.net/2445/218141
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.18176/jiaci.0995
Journal of Investigational Allergology and Clinical Immunology, 2024, vol. 35, num.3
https://doi.org/10.18176/jiaci.0995
dc.rights.none.fl_str_mv (c) Esmon Publicidad S.A., 2024
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Esmon Publicidad S.A., 2024
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 27 p.
application/pdf
dc.publisher.none.fl_str_mv Esmon Publicidad S.A.
publisher.none.fl_str_mv Esmon Publicidad S.A.
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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