α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes

The developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding a-tocopherol (0, 100, 200 and 400 mu M) during IVM on putative transcripts involved in antioxidant def...

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Detalhes bibliográficos
Autores: Arias Álvarez, María, García García, Rosa María, López-Tello, Jorge, Rebollar, Pilar, Gutiérrez-Adán, Alfonso, Lorenzo González, Pedro Luis
Tipo de documento: artigo
Data de publicação:2018
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/93700
Acesso em linha:https://hdl.handle.net/20.500.14352/93700
Access Level:Acceso aberto
Palavra-chave:636.08
Blastocyst rate
Cell death
Laboratory animal
Oocyte maturation
Redox status
Vitamin E.
Producción animal
Fisiología veterinaria
Biología celular (Biología)
Biología molecular (Biología)
3104 Producción Animal
2401.13 Fisiología Animal
2415 Biología Molecular
2407 Biología Celular
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oai_identifier_str oai:docta.ucm.es:20.500.14352/93700
network_acronym_str ES
network_name_str España
repository_id_str
spelling α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytesArias Álvarez, MaríaGarcía García, Rosa MaríaLópez-Tello, JorgeRebollar, PilarGutiérrez-Adán, AlfonsoLorenzo González, Pedro Luis636.08Blastocyst rateCell deathLaboratory animalOocyte maturationRedox statusVitamin E.Producción animalFisiología veterinariaBiología celular (Biología)Biología molecular (Biología)3104 Producción Animal2401.13 Fisiología Animal2415 Biología Molecular2407 Biología CelularThe developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding a-tocopherol (0, 100, 200 and 400 mu M) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosis tumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus-oocyte complexes (COCs) after IVM with 100 mu M alpha-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100 mu M alpha-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100 mu M alpha-tocopherol was included in the IVM medium, but remained low compared with in vivomatured oocytes. In conclusion, the addition of 100 mu M alpha-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.CSIRO PublishingUniversidad Complutense de Madrid20182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93700reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/937002026-06-02T12:44:21Z
dc.title.none.fl_str_mv α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
title α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
spellingShingle α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
Arias Álvarez, María
636.08
Blastocyst rate
Cell death
Laboratory animal
Oocyte maturation
Redox status
Vitamin E.
Producción animal
Fisiología veterinaria
Biología celular (Biología)
Biología molecular (Biología)
3104 Producción Animal
2401.13 Fisiología Animal
2415 Biología Molecular
2407 Biología Celular
title_short α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
title_full α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
title_fullStr α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
title_full_unstemmed α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
title_sort α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
dc.creator.none.fl_str_mv Arias Álvarez, María
García García, Rosa María
López-Tello, Jorge
Rebollar, Pilar
Gutiérrez-Adán, Alfonso
Lorenzo González, Pedro Luis
author Arias Álvarez, María
author_facet Arias Álvarez, María
García García, Rosa María
López-Tello, Jorge
Rebollar, Pilar
Gutiérrez-Adán, Alfonso
Lorenzo González, Pedro Luis
author_role author
author2 García García, Rosa María
López-Tello, Jorge
Rebollar, Pilar
Gutiérrez-Adán, Alfonso
Lorenzo González, Pedro Luis
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.08
Blastocyst rate
Cell death
Laboratory animal
Oocyte maturation
Redox status
Vitamin E.
Producción animal
Fisiología veterinaria
Biología celular (Biología)
Biología molecular (Biología)
3104 Producción Animal
2401.13 Fisiología Animal
2415 Biología Molecular
2407 Biología Celular
topic 636.08
Blastocyst rate
Cell death
Laboratory animal
Oocyte maturation
Redox status
Vitamin E.
Producción animal
Fisiología veterinaria
Biología celular (Biología)
Biología molecular (Biología)
3104 Producción Animal
2401.13 Fisiología Animal
2415 Biología Molecular
2407 Biología Celular
description The developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding a-tocopherol (0, 100, 200 and 400 mu M) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosis tumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus-oocyte complexes (COCs) after IVM with 100 mu M alpha-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100 mu M alpha-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100 mu M alpha-tocopherol was included in the IVM medium, but remained low compared with in vivomatured oocytes. In conclusion, the addition of 100 mu M alpha-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/93700
url https://hdl.handle.net/20.500.14352/93700
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv CSIRO Publishing
publisher.none.fl_str_mv CSIRO Publishing
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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