Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions

Background: Light/dark cycles are probably the most important environmental signals that regulate plant development. Light is essential for photosynthesis, but an excess, in combination with the unavoidable presence of atmospheric oxygen inside the chloroplast, leads to excessive reactive oxygen spe...

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Autores: Valero, Edelmira, Macià, Hermenegilda, Martínez de la Fuente Martínez, Ildefonso Abel, Hernández, José Antonio, González Sánchez, María Isabel, García Carmona, Francisco
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/32383
Acceso en línea:http://hdl.handle.net/10810/32383
Access Level:acceso abierto
Palabra clave:light/dark cycles
ascorbate-glutathione cycle
computer simulation
oxidative stress
reactive oxygen species
chloroplast
superoxide-dismutase
monodehydroascorbate reductase
dehydroascorbate reductase
steady-state
acid
stress
spinach
enzymes
leaves
oxygen
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spelling Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark ConditionsValero, EdelmiraMacià, HermenegildaMartínez de la Fuente Martínez, Ildefonso AbelHernández, José AntonioGonzález Sánchez, María IsabelGarcía Carmona, Franciscolight/dark cyclesascorbate-glutathione cyclecomputer simulationoxidative stressreactive oxygen specieschloroplastsuperoxide-dismutasemonodehydroascorbate reductasedehydroascorbate reductasesteady-stateacidstressspinachenzymesleavesoxygenBackground: Light/dark cycles are probably the most important environmental signals that regulate plant development. Light is essential for photosynthesis, but an excess, in combination with the unavoidable presence of atmospheric oxygen inside the chloroplast, leads to excessive reactive oxygen species production. Among the defense mechanisms that activate plants to cope with environmental stress situations, it is worth noting the ascorbate-glutathione cycle, a complex metabolic pathway in which a variety of photochemical, chemical and enzymatic steps are involved. Results: We herein studied the dynamic behavior of this pathway under light/dark conditions and for several consecutive days. For this purpose, a mathematical model was developed including a variable electron source with a rate law proportional to the intensity of solar irradiance during the photoperiod, and which is continuously turned off at night and on again the next day. The model is defined by a nonlinear system of ordinary differential equations with an on/off time-dependent input, including a parameter to simulate the fact that the photoperiod length is not constant throughout the year, and which takes into account the particular experimental kinetics of each enzyme involved in the pathway. Unlike previous models, which have only provided steady-state solutions, the present model is able to simulate diurnal fluctuations in the metabolite concentrations, fluxes and enzymatic rates involved in the network. Conclusions: The obtained results are broadly consistent with experimental observations and highlight the key role played by ascorbate recycling for plants to adapt to their surrounding environment. This approach provides a new strategy to in vivo studies to analyze plant defense mechanisms against oxidative stress induced by external changes, which can also be extrapolated to other complex metabolic pathways to constitute a useful tool to the scientific community in general.This work was funded by the Spanish Ministry of Economy and Competitiveness (MINECO, http://www.mineco.gob.es/portal/site/mineco/idi), Project No. BFU2013-44095-P (cofunded with FEDER funds, EU), and by the Junta de Comunidades de Castilla-La Mancha (http://www.educa.jccm.es/idiuniv/es), Project No. PEII-2014-014-A (cofunded with FEDER funds, EU). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Biomed Central201920192016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/32383reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/BFU2013-44095-P/https://bmcsystbiol.biomedcentral.com/articles/10.1186/s12918-015-0239-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/oai:addi.ehu.eus:10810/323832026-06-18T09:23:17Z
dc.title.none.fl_str_mv Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
title Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
spellingShingle Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
Valero, Edelmira
light/dark cycles
ascorbate-glutathione cycle
computer simulation
oxidative stress
reactive oxygen species
chloroplast
superoxide-dismutase
monodehydroascorbate reductase
dehydroascorbate reductase
steady-state
acid
stress
spinach
enzymes
leaves
oxygen
title_short Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
title_full Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
title_fullStr Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
title_full_unstemmed Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
title_sort Modeling the Ascorbate-Glutathione Cycle in Chloroplasts under Light/Dark Conditions
dc.creator.none.fl_str_mv Valero, Edelmira
Macià, Hermenegilda
Martínez de la Fuente Martínez, Ildefonso Abel
Hernández, José Antonio
González Sánchez, María Isabel
García Carmona, Francisco
author Valero, Edelmira
author_facet Valero, Edelmira
Macià, Hermenegilda
Martínez de la Fuente Martínez, Ildefonso Abel
Hernández, José Antonio
González Sánchez, María Isabel
García Carmona, Francisco
author_role author
author2 Macià, Hermenegilda
Martínez de la Fuente Martínez, Ildefonso Abel
Hernández, José Antonio
González Sánchez, María Isabel
García Carmona, Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv light/dark cycles
ascorbate-glutathione cycle
computer simulation
oxidative stress
reactive oxygen species
chloroplast
superoxide-dismutase
monodehydroascorbate reductase
dehydroascorbate reductase
steady-state
acid
stress
spinach
enzymes
leaves
oxygen
topic light/dark cycles
ascorbate-glutathione cycle
computer simulation
oxidative stress
reactive oxygen species
chloroplast
superoxide-dismutase
monodehydroascorbate reductase
dehydroascorbate reductase
steady-state
acid
stress
spinach
enzymes
leaves
oxygen
description Background: Light/dark cycles are probably the most important environmental signals that regulate plant development. Light is essential for photosynthesis, but an excess, in combination with the unavoidable presence of atmospheric oxygen inside the chloroplast, leads to excessive reactive oxygen species production. Among the defense mechanisms that activate plants to cope with environmental stress situations, it is worth noting the ascorbate-glutathione cycle, a complex metabolic pathway in which a variety of photochemical, chemical and enzymatic steps are involved. Results: We herein studied the dynamic behavior of this pathway under light/dark conditions and for several consecutive days. For this purpose, a mathematical model was developed including a variable electron source with a rate law proportional to the intensity of solar irradiance during the photoperiod, and which is continuously turned off at night and on again the next day. The model is defined by a nonlinear system of ordinary differential equations with an on/off time-dependent input, including a parameter to simulate the fact that the photoperiod length is not constant throughout the year, and which takes into account the particular experimental kinetics of each enzyme involved in the pathway. Unlike previous models, which have only provided steady-state solutions, the present model is able to simulate diurnal fluctuations in the metabolite concentrations, fluxes and enzymatic rates involved in the network. Conclusions: The obtained results are broadly consistent with experimental observations and highlight the key role played by ascorbate recycling for plants to adapt to their surrounding environment. This approach provides a new strategy to in vivo studies to analyze plant defense mechanisms against oxidative stress induced by external changes, which can also be extrapolated to other complex metabolic pathways to constitute a useful tool to the scientific community in general.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/32383
url http://hdl.handle.net/10810/32383
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/BFU2013-44095-P/
https://bmcsystbiol.biomedcentral.com/articles/10.1186/s12918-015-0239-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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