The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits

This study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized t...

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Autores: Morataya Reyes, Michelle|||0000-0001-9620-2069, Villacorta, Aliro|||0000-0003-2737-4232, Gutierrez Garcia, Javier|||0009-0007-7376-8933, Egea, Raquel|||0000-0002-6201-4112, Martín Pérez, Joan|||0009-0006-9137-3604, Barguilla, Irene|||0000-0003-1081-4457, Marcos Dauder, Ricardo|||0000-0001-7891-357X, Hernández Bonilla, Alba|||0000-0001-6938-1233
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:311524
Acceso en línea:https://ddd.uab.cat/record/311524
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2025.138359
Access Level:acceso abierto
Palabra clave:Nanoplastics
Cigarette smoke condensate
Long-term co-exposure
Beas-2B cells
Carcinogenic traits
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spelling The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traitsMorataya Reyes, Michelle|||0000-0001-9620-2069Villacorta, Aliro|||0000-0003-2737-4232Gutierrez Garcia, Javier|||0009-0007-7376-8933Egea, Raquel|||0000-0002-6201-4112Martín Pérez, Joan|||0009-0006-9137-3604Barguilla, Irene|||0000-0003-1081-4457Marcos Dauder, Ricardo|||0000-0001-7891-357XHernández Bonilla, Alba|||0000-0001-6938-1233NanoplasticsCigarette smoke condensateLong-term co-exposureBeas-2B cellsCarcinogenic traitsThis study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized to simulate environmental exposure conditions; and a comprehensive battery of assays was employed to assess genotoxicity, cellular transformation, and invasiveness. It was observed that, compared to passage control and individual exposures, co-exposure to PET-NPLs and CSC exacerbates oxidative stress, genotoxicity, and tumorigenic transformation, as evidenced by increased DNA damage, colony formation in soft agar, and enhanced cell migration and invasion. Transcriptomic analysis revealed a shift in cellular stress regulation including the upregulation of stress-response genes, including SLC7A11, NQO1, and HSPA1A, which are linked to oxidative stress adaptation and tumor survival. At the same time, key tumor-suppressor genes, such as LOX, and FN1, were significantly downregulated, promoting cellular transformation and invasiveness. These results provide compelling evidence that the combination of PET-NPLs and CSC enhances carcinogenic traits through oxidative stress, genomic instability, and disruption of tumor-suppressive pathways. This study underscores the importance of evaluating the synergistic effects of combined environmental exposures and their implications for human health. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/311524https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2025.138359reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2023/BP-00212European Commission https://doi.org/10.13039/501100000780 965196Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3115242026-06-06T12:50:31Z
dc.title.none.fl_str_mv The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
title The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
spellingShingle The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
Morataya Reyes, Michelle|||0000-0001-9620-2069
Nanoplastics
Cigarette smoke condensate
Long-term co-exposure
Beas-2B cells
Carcinogenic traits
title_short The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
title_full The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
title_fullStr The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
title_full_unstemmed The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
title_sort The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits
dc.creator.none.fl_str_mv Morataya Reyes, Michelle|||0000-0001-9620-2069
Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Martín Pérez, Joan|||0009-0006-9137-3604
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author Morataya Reyes, Michelle|||0000-0001-9620-2069
author_facet Morataya Reyes, Michelle|||0000-0001-9620-2069
Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Martín Pérez, Joan|||0009-0006-9137-3604
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author_role author
author2 Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Martín Pérez, Joan|||0009-0006-9137-3604
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanoplastics
Cigarette smoke condensate
Long-term co-exposure
Beas-2B cells
Carcinogenic traits
topic Nanoplastics
Cigarette smoke condensate
Long-term co-exposure
Beas-2B cells
Carcinogenic traits
description This study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized to simulate environmental exposure conditions; and a comprehensive battery of assays was employed to assess genotoxicity, cellular transformation, and invasiveness. It was observed that, compared to passage control and individual exposures, co-exposure to PET-NPLs and CSC exacerbates oxidative stress, genotoxicity, and tumorigenic transformation, as evidenced by increased DNA damage, colony formation in soft agar, and enhanced cell migration and invasion. Transcriptomic analysis revealed a shift in cellular stress regulation including the upregulation of stress-response genes, including SLC7A11, NQO1, and HSPA1A, which are linked to oxidative stress adaptation and tumor survival. At the same time, key tumor-suppressor genes, such as LOX, and FN1, were significantly downregulated, promoting cellular transformation and invasiveness. These results provide compelling evidence that the combination of PET-NPLs and CSC enhances carcinogenic traits through oxidative stress, genomic instability, and disruption of tumor-suppressive pathways. This study underscores the importance of evaluating the synergistic effects of combined environmental exposures and their implications for human health.
publishDate 2025
dc.date.none.fl_str_mv 2
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/311524
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2025.138359
url https://ddd.uab.cat/record/311524
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2025.138359
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2023/BP-00212
European Commission https://doi.org/10.13039/501100000780 965196
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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