Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

[EN] Background: The phytopathogenic fungus Diplodia seriata, whose genome remains unsequenced, produces severe infections in fruit trees (fruit blight) and grapevines. In this crop is recognized as one of the most prominent pathogens involved in grapevine trunk disease (or grapevine decline). This...

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Authors: Cobos Román, Rebeca, Barreiro Méndez, Carlos, Mateos, Rosa María, Rubio Coque, Juan José
Format: article
Status:Versión aceptada para publicación
Publication Date:2010
Country:España
Institution:Universidad Rey Juan Carlos
Repository:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/17823
Online Access:https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-46
https://hdl.handle.net/10612/17823
Access Level:Open access
Keyword:Biología
Biotecnología
Grapevine trunck diseases
Diplodia seriata
Proteomics
2414.06 Hongos
2415 Biología Molecular
2417.09 Fitopatología
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oai_identifier_str oai:buleria.unileon.es:10612/17823
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine declineCobos Román, RebecaBarreiro Méndez, CarlosMateos, Rosa MaríaRubio Coque, Juan JoséBiologíaBiotecnologíaGrapevine trunck diseasesDiplodia seriataProteomics2414.06 Hongos2415 Biología Molecular2417.09 Fitopatología[EN] Background: The phytopathogenic fungus Diplodia seriata, whose genome remains unsequenced, produces severe infections in fruit trees (fruit blight) and grapevines. In this crop is recognized as one of the most prominent pathogens involved in grapevine trunk disease (or grapevine decline). This pathology can result in the death of adult plants and therefore it produces severe economical losses all around the world. To date no genes or proteins have been characterized in D. seriata that are involved in the pathogenicity process. In an effort to help identify potential gene products associated with pathogenicity and to gain a better understanding of the biology of D. seriata, we initiated a proteome-level study of the fungal mycelia and secretome. Results: Intracellular and secreted proteins from D. seriata collected from liquid cultures were separated using twodimensional gel electrophoresis. About 550 cytoplasmic proteins were reproducibly present in 3 independent extractions, being 53 identified by peptide mass fingerprinting and tandem mass spectrometry. The secretome analysis showed 75 secreted proteins reproducibly present in 3 biological replicates, being 16 identified. Several of the proteins had been previously identified as virulence factors in other fungal strains, although their contribution to pathogenicity in D. seriata remained to be analyzed. When D. seriata was grown in a medium supplemented with carboxymethylcellulose, 3 proteins were up-regulated and 30 down-regulated. Within the up-regulated proteins, two were identified as alcohol dehydrogenase and mitochondrial peroxyrredoxin-1, suggesting that they could play a significant role in the pathogenicity process. As for the 30 down-regulated proteins, 9 were identified being several of them involved in carbohydrate metabolism. Conclusions: This study is the first report on proteomics on D. seriata. The proteomic data obtained will be important to understand the pathogenicity process. In fact, several of the identified proteins have been reported as pathogenicity factors in other phytopathogenic fungi. Moreover, this proteomic analysis supposes a useful basis for deepening into D. seriata knowledge and will contribute to the development of the molecular biology of this fungal strain as it has been demonstrated by cloning the gene Prx1 encoding mitochondrial peroxiredoxin-1 of D. seriata (the first gene to be cloned in this microorganism; data not shown)SIThis work has been financed with the benefits of a Research Contract sponsored by Bodegas Vega Sicilia S.A.U. (Valbuena de Duero, Valladolid, Spain). R. Cobos and R.M. Mateos were supported by a Research Contract financed by Bodegas Vega Sicilia. C. Barreiro was supported by the Program ‘Personal Técnico de Apoyo’ of the Ministry of Science and Innovation of Spain and the European Social Fund (ESF) (PTA-2003-01-00689). The technical support of M.D. Gutiérrez (Universidad Complutense de Madrid, Spain) is acknowledged.SpringerMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-46https://hdl.handle.net/10612/17823reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MICINN/Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica.Personal Técnico de Apoyo/PTA-2003-01-00689info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/178232026-06-24T12:43:27Z
dc.title.none.fl_str_mv Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
title Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
spellingShingle Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
Cobos Román, Rebeca
Biología
Biotecnología
Grapevine trunck diseases
Diplodia seriata
Proteomics
2414.06 Hongos
2415 Biología Molecular
2417.09 Fitopatología
title_short Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
title_full Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
title_fullStr Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
title_full_unstemmed Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
title_sort Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
dc.creator.none.fl_str_mv Cobos Román, Rebeca
Barreiro Méndez, Carlos
Mateos, Rosa María
Rubio Coque, Juan José
author Cobos Román, Rebeca
author_facet Cobos Román, Rebeca
Barreiro Méndez, Carlos
Mateos, Rosa María
Rubio Coque, Juan José
author_role author
author2 Barreiro Méndez, Carlos
Mateos, Rosa María
Rubio Coque, Juan José
author2_role author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Biotecnología
Grapevine trunck diseases
Diplodia seriata
Proteomics
2414.06 Hongos
2415 Biología Molecular
2417.09 Fitopatología
topic Biología
Biotecnología
Grapevine trunck diseases
Diplodia seriata
Proteomics
2414.06 Hongos
2415 Biología Molecular
2417.09 Fitopatología
description [EN] Background: The phytopathogenic fungus Diplodia seriata, whose genome remains unsequenced, produces severe infections in fruit trees (fruit blight) and grapevines. In this crop is recognized as one of the most prominent pathogens involved in grapevine trunk disease (or grapevine decline). This pathology can result in the death of adult plants and therefore it produces severe economical losses all around the world. To date no genes or proteins have been characterized in D. seriata that are involved in the pathogenicity process. In an effort to help identify potential gene products associated with pathogenicity and to gain a better understanding of the biology of D. seriata, we initiated a proteome-level study of the fungal mycelia and secretome. Results: Intracellular and secreted proteins from D. seriata collected from liquid cultures were separated using twodimensional gel electrophoresis. About 550 cytoplasmic proteins were reproducibly present in 3 independent extractions, being 53 identified by peptide mass fingerprinting and tandem mass spectrometry. The secretome analysis showed 75 secreted proteins reproducibly present in 3 biological replicates, being 16 identified. Several of the proteins had been previously identified as virulence factors in other fungal strains, although their contribution to pathogenicity in D. seriata remained to be analyzed. When D. seriata was grown in a medium supplemented with carboxymethylcellulose, 3 proteins were up-regulated and 30 down-regulated. Within the up-regulated proteins, two were identified as alcohol dehydrogenase and mitochondrial peroxyrredoxin-1, suggesting that they could play a significant role in the pathogenicity process. As for the 30 down-regulated proteins, 9 were identified being several of them involved in carbohydrate metabolism. Conclusions: This study is the first report on proteomics on D. seriata. The proteomic data obtained will be important to understand the pathogenicity process. In fact, several of the identified proteins have been reported as pathogenicity factors in other phytopathogenic fungi. Moreover, this proteomic analysis supposes a useful basis for deepening into D. seriata knowledge and will contribute to the development of the molecular biology of this fungal strain as it has been demonstrated by cloning the gene Prx1 encoding mitochondrial peroxiredoxin-1 of D. seriata (the first gene to be cloned in this microorganism; data not shown)
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-46
https://hdl.handle.net/10612/17823
url https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-46
https://hdl.handle.net/10612/17823
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica.Personal Técnico de Apoyo/PTA-2003-01-00689
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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