Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria

The potential of probiotic bacteria to produce prebiotic oligosaccharides by transgalactosylation has been minimally studied. In this work, we screened the β-galactosidase (β-gal) activity of dairy propionibacteria (PAB) isolated from Argentinean foods to select strains for the synthesis of oligosac...

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Autores: Sabater, Carlos, Fara, Agustina, Palacios, Jorge, Corzo, Nieves, Requena, Teresa, Montilla, Antonia, Zárate, Gabriela
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/174200
Acesso em linha:http://hdl.handle.net/10261/174200
Access Level:acceso abierto
Palavra-chave:Prebiotics
Transgalactosylation
Oligosaccharides
β-galactosidase
Propionibacteria
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spelling Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteriaSabater, CarlosFara, AgustinaPalacios, JorgeCorzo, NievesRequena, TeresaMontilla, AntoniaZárate, GabrielaPrebioticsTransgalactosylationOligosaccharidesβ-galactosidasePropionibacteriaThe potential of probiotic bacteria to produce prebiotic oligosaccharides by transgalactosylation has been minimally studied. In this work, we screened the β-galactosidase (β-gal) activity of dairy propionibacteria (PAB) isolated from Argentinean foods to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu). PAB, when grown in a medium with lactose as a carbon source, were disrupted, and the cell-free extracts were assayed for β-gal activity. Nine strains grew on lactose and showed β-gal activities from 0.27 to 2.60 U mL. Propionibacterium acidipropionici LET 120, the strain with the highest activity, was able to synthesize, using 30% lactose and lactulose at pH 6.5 and 45 °C, 26.8% of LET 120-GOS and 26.1% of LET 120-OsLu after 24 h. When they were tested as carbon sources for growth, P. acidipropionici LET 120 attained higher biomasses, μ and β-gal activities at the expense of Aspergillus oryzae-OsLu, Vivinal-GOS and lactulose compared to lactose or glucose. In addition, LET 120-GOS and LET 120-OsLu synthesized by PAB were prebiotic for some probiotic strains. For the first time, our results show the production of GOS and OsLu by dairy PAB, and these results encourage further studies on the optimization of the synthesis and structure characterization of the obtained oligosaccharides.This work was supported by PICT 2014-3583 from ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica, Argentina), Project I-COOP_2014_COOPB20099 and EMHE-CSIC 2017 MHE-200047 from CSIC (Consejo Superior de Investigaciones Científicas, España); and AGL2017-84614-C2-1-R from MICINN. Carlos Sabater thanks his FPU Predoc contract from Spanish MECD (FPU14/03619).Peer reviewedPeer ReviewedElsevierAgencia Nacional de Promoción Científica y Tecnológica (Argentina)Consejo Superior de Investigaciones Científicas (España)Ministerio de Educación, Cultura y Deporte (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionapplication/pdfhttp://hdl.handle.net/10261/174200reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-1-Rhttps://doi.org/10.1016/j.fm.2018.08.014Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1742002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
title Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
spellingShingle Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
Sabater, Carlos
Prebiotics
Transgalactosylation
Oligosaccharides
β-galactosidase
Propionibacteria
title_short Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
title_full Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
title_fullStr Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
title_full_unstemmed Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
title_sort Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
dc.creator.none.fl_str_mv Sabater, Carlos
Fara, Agustina
Palacios, Jorge
Corzo, Nieves
Requena, Teresa
Montilla, Antonia
Zárate, Gabriela
author Sabater, Carlos
author_facet Sabater, Carlos
Fara, Agustina
Palacios, Jorge
Corzo, Nieves
Requena, Teresa
Montilla, Antonia
Zárate, Gabriela
author_role author
author2 Fara, Agustina
Palacios, Jorge
Corzo, Nieves
Requena, Teresa
Montilla, Antonia
Zárate, Gabriela
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Nacional de Promoción Científica y Tecnológica (Argentina)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Educación, Cultura y Deporte (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Prebiotics
Transgalactosylation
Oligosaccharides
β-galactosidase
Propionibacteria
topic Prebiotics
Transgalactosylation
Oligosaccharides
β-galactosidase
Propionibacteria
description The potential of probiotic bacteria to produce prebiotic oligosaccharides by transgalactosylation has been minimally studied. In this work, we screened the β-galactosidase (β-gal) activity of dairy propionibacteria (PAB) isolated from Argentinean foods to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu). PAB, when grown in a medium with lactose as a carbon source, were disrupted, and the cell-free extracts were assayed for β-gal activity. Nine strains grew on lactose and showed β-gal activities from 0.27 to 2.60 U mL. Propionibacterium acidipropionici LET 120, the strain with the highest activity, was able to synthesize, using 30% lactose and lactulose at pH 6.5 and 45 °C, 26.8% of LET 120-GOS and 26.1% of LET 120-OsLu after 24 h. When they were tested as carbon sources for growth, P. acidipropionici LET 120 attained higher biomasses, μ and β-gal activities at the expense of Aspergillus oryzae-OsLu, Vivinal-GOS and lactulose compared to lactose or glucose. In addition, LET 120-GOS and LET 120-OsLu synthesized by PAB were prebiotic for some probiotic strains. For the first time, our results show the production of GOS and OsLu by dairy PAB, and these results encourage further studies on the optimization of the synthesis and structure characterization of the obtained oligosaccharides.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/174200
url http://hdl.handle.net/10261/174200
dc.language.none.fl_str_mv Inglés
Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-1-R
https://doi.org/10.1016/j.fm.2018.08.014
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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