Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum

This article belongs to the Special Issue Advances in Plant Pathology of Viticulture—2nd Edition

Detalhes bibliográficos
Autores: Gómez-Garay, Aránzazu, Tello, M. L., Astudillo Calderón, Sergio, Pintos, Beatriz
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::282365fbfdf31c860d1a6b26f920f339
Acesso em linha:http://hdl.handle.net/10261/426977
Access Level:acceso abierto
Palavra-chave:Trichoderma
Silver nanoparticles
Green synthesis
Fungal redox metabolism
Biocontrol
Grapevine trunk diseases
Neofusicoccum parvum
Nanobiotechnology
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spelling Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvumGómez-Garay, AránzazuTello, M. L.Astudillo Calderón, SergioPintos, BeatrizTrichodermaSilver nanoparticlesGreen synthesisFungal redox metabolismBiocontrolGrapevine trunk diseasesNeofusicoccum parvumNanobiotechnologyThis article belongs to the Special Issue Advances in Plant Pathology of Viticulture—2nd EditionGrapevine trunk diseases, particularly those caused by Neofusicoccum parvum, represent a major threat to vineyard productivity and are increasingly difficult to control with conventional fungicides. Green synthesis of silver nanoparticles (AgNPs) using biocontrol fungi offers a promising alternative, but the factors governing the efficiency and bioactivity of biogenic nanoparticles remain poorly understood. Here, three Trichoderma species (T. harzianum, T. asperellum and T. virens) were evaluated as biological nanofactories for AgNP production. Cell-free fungal filtrates were used to synthesize AgNPs, which were characterized by UV–visible spectrophotometry, Dynamic Light Scattering (DLS) and transmission electron microscopy, while fungal redox metabolism was assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assays and HPLC profiling of extracellular metabolites. AgNPs were tested against two isolates of N. parvum in vitro. The Trichoderma strains differed markedly in nanoparticle yield, size and antifungal activity, with T. harzianum T0 producing the highest amounts of small, well-dispersed AgNPs that exerted a strong fungistatic effect on N. parvum. Nanoparticle production correlated with antioxidant capacity and the abundance of redox-active metabolites. Integration of these parameters into a Fungal Nanofactory Efficiency Index (FNEI) revealed that nanoparticle bioactivity depends on both dose and biological origin. These results demonstrate that fungal metabolism is a key determinant of biogenic nanoparticle performance and identify Trichoderma as a platform for sustainable nanotechnology-based control of grapevine trunk pathogens.This research was funded by INIA, Ministry of Science, Innovation and Universities, Madrid, Spain, Project number RTA2015-0015-C02-02.Peer reviewedMultidisciplinary Digital Publishing InstituteCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2026202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/426977reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/agronomy16060663Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::282365fbfdf31c860d1a6b26f920f3392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
title Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
spellingShingle Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
Gómez-Garay, Aránzazu
Trichoderma
Silver nanoparticles
Green synthesis
Fungal redox metabolism
Biocontrol
Grapevine trunk diseases
Neofusicoccum parvum
Nanobiotechnology
title_short Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
title_full Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
title_fullStr Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
title_full_unstemmed Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
title_sort Trichoderma as a Biological Nanofactory: Metabolic Control of Silver Nanoparticle Biosynthesis and Activity Against the Grapevine Trunk Pathogen Neofusicoccum parvum
dc.creator.none.fl_str_mv Gómez-Garay, Aránzazu
Tello, M. L.
Astudillo Calderón, Sergio
Pintos, Beatriz
author Gómez-Garay, Aránzazu
author_facet Gómez-Garay, Aránzazu
Tello, M. L.
Astudillo Calderón, Sergio
Pintos, Beatriz
author_role author
author2 Tello, M. L.
Astudillo Calderón, Sergio
Pintos, Beatriz
author2_role author
author
author
dc.contributor.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Trichoderma
Silver nanoparticles
Green synthesis
Fungal redox metabolism
Biocontrol
Grapevine trunk diseases
Neofusicoccum parvum
Nanobiotechnology
topic Trichoderma
Silver nanoparticles
Green synthesis
Fungal redox metabolism
Biocontrol
Grapevine trunk diseases
Neofusicoccum parvum
Nanobiotechnology
description This article belongs to the Special Issue Advances in Plant Pathology of Viticulture—2nd Edition
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/426977
url http://hdl.handle.net/10261/426977
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/agronomy16060663
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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