A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit

[EN] BackgroundIsoprenoids are a very large class of metabolites playing a key role in plant physiological processes such as growth, stress resistance, fruit flavor, and color. In chloroplasts and chromoplasts, the diterpene compound geranylgeranyl diphosphate (GGPP) is the metabolic precursor requi...

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Autores: Zita, Wayne, Shanmugabalaji, Venkatasalam, Ezquerro-Urzanqui, Miguel, Kessler, Felix, Glauser, Gaetan, RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/212439
Acesso em linha:https://riunet.upv.es/handle/10251/212439
Access Level:acceso abierto
Palavra-chave:Geranylgeranyl diphosphate
GGPP
Geranylgeranyl monophosphate
GGP
Geranylgeranyl diphosphate synthase
GGPPS
UHPLC-MS/MS
Isoprenoid
Carotenoid
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network_acronym_str ES
network_name_str España
repository_id_str
spelling A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruitZita, WayneShanmugabalaji, VenkatasalamEzquerro-Urzanqui, MiguelKessler, FelixGlauser, GaetanRODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305Geranylgeranyl diphosphateGGPPGeranylgeranyl monophosphateGGPGeranylgeranyl diphosphate synthaseGGPPSUHPLC-MS/MSIsoprenoidCarotenoid[EN] BackgroundIsoprenoids are a very large class of metabolites playing a key role in plant physiological processes such as growth, stress resistance, fruit flavor, and color. In chloroplasts and chromoplasts, the diterpene compound geranylgeranyl diphosphate (GGPP) is the metabolic precursor required for the biosynthesis of tocopherols, plastoquinones, phylloquinone, chlorophylls, and carotenoids. Despite its key role for the plant metabolism, reports on GGPP physiological concentrations in planta have been extremely scarce.ResultsIn this study, we developed a method to quantify GGPP and its hydrolysis product geranylgeranyl monophosphate (GGP) from tomato fruit, using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Quantification was done by external calibration and the method was validated in terms of specificity, precision, accuracy, and detection and quantitation limits. We further demonstrate the validity of our approach by analysing GGPP contents in the ripe fruits of wild-type tomatoes and mutants defective in GGPP production. Finally, we also show that the sample preparation is key to prevent GGPP hydrolysis and mitigate its conversion to GGP.ConclusionOur study provides an efficient tool to investigate the metabolic fluxes required for GGPP supply and consumption in tomato fruit.This work was funded by the Swiss National Science Foundation (Project 310030_208000 to F.K.) and by the University of Neuchâtel.Springer (Biomed Central Ltd.)Instituto Universitario Mixto de Biología Molecular y Celular de PlantasUniversité de NeuchâtelSwiss National Science FoundationRepositorio Institucional de la Universitat Politècnica de València Riunet20232023-06-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/212439reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengSwiss National Science Foundation https://doi.org/10.13039/501100001711 310030_208000open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2124392026-06-13T07:49:27Z
dc.title.none.fl_str_mv A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
title A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
spellingShingle A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
Zita, Wayne
Geranylgeranyl diphosphate
GGPP
Geranylgeranyl monophosphate
GGP
Geranylgeranyl diphosphate synthase
GGPPS
UHPLC-MS/MS
Isoprenoid
Carotenoid
title_short A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
title_full A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
title_fullStr A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
title_full_unstemmed A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
title_sort A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit
dc.creator.none.fl_str_mv Zita, Wayne
Shanmugabalaji, Venkatasalam
Ezquerro-Urzanqui, Miguel
Kessler, Felix
Glauser, Gaetan
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author Zita, Wayne
author_facet Zita, Wayne
Shanmugabalaji, Venkatasalam
Ezquerro-Urzanqui, Miguel
Kessler, Felix
Glauser, Gaetan
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author_role author
author2 Shanmugabalaji, Venkatasalam
Ezquerro-Urzanqui, Miguel
Kessler, Felix
Glauser, Gaetan
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Université de Neuchâtel
Swiss National Science Foundation
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Geranylgeranyl diphosphate
GGPP
Geranylgeranyl monophosphate
GGP
Geranylgeranyl diphosphate synthase
GGPPS
UHPLC-MS/MS
Isoprenoid
Carotenoid
topic Geranylgeranyl diphosphate
GGPP
Geranylgeranyl monophosphate
GGP
Geranylgeranyl diphosphate synthase
GGPPS
UHPLC-MS/MS
Isoprenoid
Carotenoid
description [EN] BackgroundIsoprenoids are a very large class of metabolites playing a key role in plant physiological processes such as growth, stress resistance, fruit flavor, and color. In chloroplasts and chromoplasts, the diterpene compound geranylgeranyl diphosphate (GGPP) is the metabolic precursor required for the biosynthesis of tocopherols, plastoquinones, phylloquinone, chlorophylls, and carotenoids. Despite its key role for the plant metabolism, reports on GGPP physiological concentrations in planta have been extremely scarce.ResultsIn this study, we developed a method to quantify GGPP and its hydrolysis product geranylgeranyl monophosphate (GGP) from tomato fruit, using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Quantification was done by external calibration and the method was validated in terms of specificity, precision, accuracy, and detection and quantitation limits. We further demonstrate the validity of our approach by analysing GGPP contents in the ripe fruits of wild-type tomatoes and mutants defective in GGPP production. Finally, we also show that the sample preparation is key to prevent GGPP hydrolysis and mitigate its conversion to GGP.ConclusionOur study provides an efficient tool to investigate the metabolic fluxes required for GGPP supply and consumption in tomato fruit.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-06-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/212439
url https://riunet.upv.es/handle/10251/212439
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Swiss National Science Foundation https://doi.org/10.13039/501100001711 310030_208000
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer (Biomed Central Ltd.)
publisher.none.fl_str_mv Springer (Biomed Central Ltd.)
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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