Modulating oxidoreductase activity modifies the phenolic content of virgin olive oil

© 2014 Published by Elsevier Ltd. The effect of modifying polyphenol oxidase (PPO) and peroxidase (POX) activity during the extraction of virgin olive oil has been assessed in terms of its influence on the phenolic profile of the oil produced. These enzymes were modified by adding exogenous enzyme o...

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Detalhes bibliográficos
Autores: García-Rodríguez, Rosa, Romero-Segura, Carmen, Sanz, Carlos, Pérez Rubio, Ana Gracia
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/116143
Acesso em linha:http://hdl.handle.net/10261/116143
Access Level:acceso abierto
Palavra-chave:Quality
Phenolic compounds
Extraction
Virgin olive oil
Peroxidase
Polyphenol oxidase
Descrição
Resumo:© 2014 Published by Elsevier Ltd. The effect of modifying polyphenol oxidase (PPO) and peroxidase (POX) activity during the extraction of virgin olive oil has been assessed in terms of its influence on the phenolic profile of the oil produced. These enzymes were modified by adding exogenous enzyme or specific inhibitors during the milling and subsequent kneading step, studying the effect on specific phenolic compounds in the oils. PPO is the main enzyme involved in phenolic oxidation at the milling step whereas POX activity seems to be the main influence during the kneading step. The data obtained suggest it is possible to increase the nutritional and organoleptic quality of virgin olive oil by inhibiting these enzymes during olive fruit processing. Treatment with the PPO inhibitor tropolone produced a twofold increase in the phenolic fraction, which would therefore seem to be an interesting strategy to improve the nutritional and organoleptic properties of virgin olive oil.