Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda
The inefficient production of nucleopolyhedrovirus occlusion bodies (OBs) can limit the commercialization of virus-based insecticides. The production of OBs in SfMNPV-infected Spodoptera frugiperda fourth instars was compared among groups of larvae that were frozen (−20 °C) immediately following dea...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2019 |
| País: | España |
| Recursos: | Universidad Pública de Navarra |
| Repositorio: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/36007 |
| Acesso em linha: | https://hdl.handle.net/2454/36007 |
| Access Level: | acceso abierto |
| Palavra-chave: | Spodoptera frugiperda multiple nucleopolyhedrovirus Biological insecticide production Insecticidal activity qPCR OB size |
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Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperdaRamírez-Arias, Fernando G.Lasa, RodrigoMurillo Pérez, RosaNavarro de la Fuente, LauraMercado, GabrielWilliams, TrevorSpodoptera frugiperda multiple nucleopolyhedrovirusBiological insecticide productionInsecticidal activityqPCROB sizeThe inefficient production of nucleopolyhedrovirus occlusion bodies (OBs) can limit the commercialization of virus-based insecticides. The production of OBs in SfMNPV-infected Spodoptera frugiperda fourth instars was compared among groups of larvae that were frozen (−20 °C) immediately following death, or subjected to a 9-day period of post-mortem incubation at 5 °C or 15 °C. Incubation at 15 °C resulted in a ∼40% increase in total OB production and OBs per mg larval weight, compared to the −20 °C and 5 °C treatments. OBs from the 5 °C treatment were ∼10% smaller in cross sectional area and small OBs (<1 µm 2 ) were more abundant than in other treatments, possibly due to reduced post-mortem OB maturation in this treatment. SfMNPV genomic DNA in OB samples was 3.6-fold higher in the −20 °C treatment than the 5 °C treatment and 1.7-fold higher than the 15 °C treatment, possibly due to differences in the exposure of viral genomes to degradative enzymes. However, these differences did not affect the concentration-mortality relationship or speed of kill of OBs from the different treatments. The abundance of aerobic microbes increased from ∼3 × 10 7 CFU/ml in the −20 °C treatment, to approximately 2 × 10 8 and 3 × 10 8 CFU/ml in the 5 °C and 15 °C incubation treatments, respectively, similar to levels seen in other nucleopolyhedroviruses produced in insects. We conclude that post-mortem incubation at 15 °C likely involves continuing processes of virion occlusion and OB maturation that increase overall OB production without loss of insecticidal activity, although the value of this step in commercial virus insecticide production will depend on the cost of the incubation step and the value of the additional OBs produced.ElsevierAgronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta Elikadura2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2454/36007reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/360072026-06-17T12:41:47Z |
| dc.title.none.fl_str_mv |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| title |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| spellingShingle |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda Ramírez-Arias, Fernando G. Spodoptera frugiperda multiple nucleopolyhedrovirus Biological insecticide production Insecticidal activity qPCR OB size |
| title_short |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| title_full |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| title_fullStr |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| title_full_unstemmed |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| title_sort |
Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda |
| dc.creator.none.fl_str_mv |
Ramírez-Arias, Fernando G. Lasa, Rodrigo Murillo Pérez, Rosa Navarro de la Fuente, Laura Mercado, Gabriel Williams, Trevor |
| author |
Ramírez-Arias, Fernando G. |
| author_facet |
Ramírez-Arias, Fernando G. Lasa, Rodrigo Murillo Pérez, Rosa Navarro de la Fuente, Laura Mercado, Gabriel Williams, Trevor |
| author_role |
author |
| author2 |
Lasa, Rodrigo Murillo Pérez, Rosa Navarro de la Fuente, Laura Mercado, Gabriel Williams, Trevor |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Agronomía, Biotecnología y Alimentación Agronomia, Bioteknologia eta Elikadura |
| dc.subject.none.fl_str_mv |
Spodoptera frugiperda multiple nucleopolyhedrovirus Biological insecticide production Insecticidal activity qPCR OB size |
| topic |
Spodoptera frugiperda multiple nucleopolyhedrovirus Biological insecticide production Insecticidal activity qPCR OB size |
| description |
The inefficient production of nucleopolyhedrovirus occlusion bodies (OBs) can limit the commercialization of virus-based insecticides. The production of OBs in SfMNPV-infected Spodoptera frugiperda fourth instars was compared among groups of larvae that were frozen (−20 °C) immediately following death, or subjected to a 9-day period of post-mortem incubation at 5 °C or 15 °C. Incubation at 15 °C resulted in a ∼40% increase in total OB production and OBs per mg larval weight, compared to the −20 °C and 5 °C treatments. OBs from the 5 °C treatment were ∼10% smaller in cross sectional area and small OBs (<1 µm 2 ) were more abundant than in other treatments, possibly due to reduced post-mortem OB maturation in this treatment. SfMNPV genomic DNA in OB samples was 3.6-fold higher in the −20 °C treatment than the 5 °C treatment and 1.7-fold higher than the 15 °C treatment, possibly due to differences in the exposure of viral genomes to degradative enzymes. However, these differences did not affect the concentration-mortality relationship or speed of kill of OBs from the different treatments. The abundance of aerobic microbes increased from ∼3 × 10 7 CFU/ml in the −20 °C treatment, to approximately 2 × 10 8 and 3 × 10 8 CFU/ml in the 5 °C and 15 °C incubation treatments, respectively, similar to levels seen in other nucleopolyhedroviruses produced in insects. We conclude that post-mortem incubation at 15 °C likely involves continuing processes of virion occlusion and OB maturation that increase overall OB production without loss of insecticidal activity, although the value of this step in commercial virus insecticide production will depend on the cost of the incubation step and the value of the additional OBs produced. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2454/36007 |
| url |
https://hdl.handle.net/2454/36007 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra instname:Universidad Pública de Navarra |
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Universidad Pública de Navarra |
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Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
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Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
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