Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation

[EN] The effects of three treatments of fibrolytic enzymes (cellulase from Trichoderma longibrachiatum (CEL), xylanase from rumen micro-organisms (XYL) and a 1:1 mixture of CEL and XYL (MIX) on the in vitro fermentation of two samples of Pennisetum clandestinum (P1 and P2), two samples of Dichanthiu...

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Authors: Díaz Reyes, Alexey, Ranilla García, María José, Giraldo Valderrama, Luis Alfonso, Tejido, María Luisa, Carro Travieso, María Dolores
Format: article
Status:Versión enviada para evaluación y publicación
Publication Date:2014
Country:España
Institution:Universidad de León
Repository:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:dnet:buleria_____::b46ee80ac94609a2e41cd2a1b691520b
Online Access:https://onlinelibrary.wiley.com/doi/10.1111/jpn.12175
https://hdl.handle.net/10612/27978
Access Level:Open access
Keyword:Producción animal
Fibrolytic enzymes,
Tropical forages
Ruminal fermentation
Batch cultures
3109 Ciencias Veterinarias
3104 Producción Animal
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oai_identifier_str oai:dnet:buleria_____::b46ee80ac94609a2e41cd2a1b691520b
network_acronym_str ES
network_name_str España
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spelling Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentationDíaz Reyes, AlexeyRanilla García, María JoséGiraldo Valderrama, Luis AlfonsoTejido, María LuisaCarro Travieso, María DoloresProducción animalFibrolytic enzymes,Tropical foragesRuminal fermentationBatch cultures3109 Ciencias Veterinarias3104 Producción Animal[EN] The effects of three treatments of fibrolytic enzymes (cellulase from Trichoderma longibrachiatum (CEL), xylanase from rumen micro-organisms (XYL) and a 1:1 mixture of CEL and XYL (MIX) on the in vitro fermentation of two samples of Pennisetum clandestinum (P1 and P2), two samples of Dichanthium aristatum (D1 and D2) and one sample of each Acacia decurrens and Acacia mangium (A1 and A2) were investigated. The first experiment compared the effects of two methods of applying the enzymes to forages, either at the time of incubation or 24 h before, on the in vitro gas production. In general, the 24 h pre-treatment resulted in higher values of gas production rate, and this application method was chosen for a second study investigating the effects of enzymes on chemical composition and in vitro fermentation of forages. The pre-treatment with CEL for 24 h reduced (p < 0.05) the content of neutral detergent fibre (NDF) of P1, P2, D1 and D2, and that of MIX reduced the NDF content of P1 and D1, but XYL had no effect on any forage. The CEL treatment increased (p < 0.05) total volatile fatty acid (VFA) production for all forages (ranging from 8.6% to 22.7%), but in general, no effects of MIX and XYL were observed. For both P. clandestinum samples, CEL treatment reduced (p < 0.05) the molar proportion of acetate and increased (p < 0.05) that of butyrate, but only subtle changes in VFA profile were observed for the rest of forages. Under the conditions of the present experiment, the treatment of tropical forages with CEL stimulated their in vitro ruminal fermentation, but XYL did not produce any positive effect. These results showed clearly that effectiveness of enzymes varied with the incubated forage and further study is warranted to investigate specific, optimal enzyme-substrate combinations.SIThe authors wish to acknowledge the financial support received from the Junta de Castilla y León (Project LE040A05), MICINN (Project AGL2008-04707-C02-02) and the MAE-AECID (Project A/4951/06). A. Díaz and L.A. Giraldo gratefully acknowledge receipt of grants from the Spanish AECID and the Fundación Carolina respectively. M.L. Tejido gratefully acknowledges a postdoctoral contract from Spanish CSIC (JAE-doc program).Wiley‑Blackwell Publishing LtdProducción AnimalFacultad de Veterinaria2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttps://onlinelibrary.wiley.com/doi/10.1111/jpn.12175https://hdl.handle.net/10612/27978reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación/ Programa Nacional de Investigación Fundamental / AGL2008-04707-C02-02http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:buleria_____::b46ee80ac94609a2e41cd2a1b691520b2026-06-24T12:43:27Z
dc.title.none.fl_str_mv Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
title Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
spellingShingle Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
Díaz Reyes, Alexey
Producción animal
Fibrolytic enzymes,
Tropical forages
Ruminal fermentation
Batch cultures
3109 Ciencias Veterinarias
3104 Producción Animal
title_short Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
title_full Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
title_fullStr Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
title_full_unstemmed Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
title_sort Treatment of tropical forages with exogenous fibrolytic enzymes: effects on chemical composition and in vitro rumen fermentation
dc.creator.none.fl_str_mv Díaz Reyes, Alexey
Ranilla García, María José
Giraldo Valderrama, Luis Alfonso
Tejido, María Luisa
Carro Travieso, María Dolores
author Díaz Reyes, Alexey
author_facet Díaz Reyes, Alexey
Ranilla García, María José
Giraldo Valderrama, Luis Alfonso
Tejido, María Luisa
Carro Travieso, María Dolores
author_role author
author2 Ranilla García, María José
Giraldo Valderrama, Luis Alfonso
Tejido, María Luisa
Carro Travieso, María Dolores
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producción Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Producción animal
Fibrolytic enzymes,
Tropical forages
Ruminal fermentation
Batch cultures
3109 Ciencias Veterinarias
3104 Producción Animal
topic Producción animal
Fibrolytic enzymes,
Tropical forages
Ruminal fermentation
Batch cultures
3109 Ciencias Veterinarias
3104 Producción Animal
description [EN] The effects of three treatments of fibrolytic enzymes (cellulase from Trichoderma longibrachiatum (CEL), xylanase from rumen micro-organisms (XYL) and a 1:1 mixture of CEL and XYL (MIX) on the in vitro fermentation of two samples of Pennisetum clandestinum (P1 and P2), two samples of Dichanthium aristatum (D1 and D2) and one sample of each Acacia decurrens and Acacia mangium (A1 and A2) were investigated. The first experiment compared the effects of two methods of applying the enzymes to forages, either at the time of incubation or 24 h before, on the in vitro gas production. In general, the 24 h pre-treatment resulted in higher values of gas production rate, and this application method was chosen for a second study investigating the effects of enzymes on chemical composition and in vitro fermentation of forages. The pre-treatment with CEL for 24 h reduced (p < 0.05) the content of neutral detergent fibre (NDF) of P1, P2, D1 and D2, and that of MIX reduced the NDF content of P1 and D1, but XYL had no effect on any forage. The CEL treatment increased (p < 0.05) total volatile fatty acid (VFA) production for all forages (ranging from 8.6% to 22.7%), but in general, no effects of MIX and XYL were observed. For both P. clandestinum samples, CEL treatment reduced (p < 0.05) the molar proportion of acetate and increased (p < 0.05) that of butyrate, but only subtle changes in VFA profile were observed for the rest of forages. Under the conditions of the present experiment, the treatment of tropical forages with CEL stimulated their in vitro ruminal fermentation, but XYL did not produce any positive effect. These results showed clearly that effectiveness of enzymes varied with the incubated forage and further study is warranted to investigate specific, optimal enzyme-substrate combinations.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/10.1111/jpn.12175
https://hdl.handle.net/10612/27978
url https://onlinelibrary.wiley.com/doi/10.1111/jpn.12175
https://hdl.handle.net/10612/27978
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación/ Programa Nacional de Investigación Fundamental / AGL2008-04707-C02-02
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley‑Blackwell Publishing Ltd
publisher.none.fl_str_mv Wiley‑Blackwell Publishing Ltd
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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