Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family

Cyclin-dependent kinases from the Cdk5/Pho85 family are thought to play important roles in morphogenesis in organisms as diverse as yeast and humans. Here we used the corn smut fungus Ustilago maydis to address the role of Cdk5/Pho85 kinases in the morphogenesis and virulence of dimorphic phytopatho...

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Authors: Castillo-Lluva, Sonia, Álvarez-Tabarés, Isabel, Weber, Isabella, Steinberg, Gero, Pérez-Martín, José
Format: article
Status:Versión aceptada para publicación
Publication Date:2007
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/339636
Online Access:http://hdl.handle.net/10261/339636
Access Level:Open access
Keyword:Cdk5
Corn smut
Cell polarity
Morphogenesis
Ustilago maydis
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spelling Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 familyCastillo-Lluva, SoniaÁlvarez-Tabarés, IsabelWeber, IsabellaSteinberg, GeroPérez-Martín, JoséCdk5Corn smutCell polarityMorphogenesisUstilago maydisCyclin-dependent kinases from the Cdk5/Pho85 family are thought to play important roles in morphogenesis in organisms as diverse as yeast and humans. Here we used the corn smut fungus Ustilago maydis to address the role of Cdk5/Pho85 kinases in the morphogenesis and virulence of dimorphic phytopathogens. We found that Cdk5 is essential for growth in U. maydis. A temperature-sensitive cdk5 mutant caused cell wall and morphology defects at the restrictive temperature. Actin patches labeled with a fimbrin-GFP fusion protein were delocalized and a GFP-Myo5 fusion was directed towards the growing cell pole and rapidly dissociated from the tip. These defects were found to be due to an impairment in the maintenance of cell polarity. Our results indicated that Cdk5 is required for the activity of Rac1, probably at the level of the localization of its GEF, Cdc24. Cdk5 was required for full virulence, probably because mutant cells are unable to sustain the dramatic polar growth required for the formation of the infective structures. These results support a major role for morphogenesis in the virulence program of dimorphic fungi.The authors thank P. Sudbery for critical reading of the manuscript. The helpful suggestions of two anonymous reviewers are also thanked. This work was supported by Grants to J.P.M. from the Spanish government (BIO2005-02998) and EU (MRTN-CT-2005-019277). The authors declare no competing financial interests.Company of BiologistsMinisterio de Educación y Ciencia (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320072023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/339636reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jcs.005314Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3396362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
title Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
spellingShingle Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
Castillo-Lluva, Sonia
Cdk5
Corn smut
Cell polarity
Morphogenesis
Ustilago maydis
title_short Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
title_full Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
title_fullStr Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
title_full_unstemmed Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
title_sort Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5-Pho85 family
dc.creator.none.fl_str_mv Castillo-Lluva, Sonia
Álvarez-Tabarés, Isabel
Weber, Isabella
Steinberg, Gero
Pérez-Martín, José
author Castillo-Lluva, Sonia
author_facet Castillo-Lluva, Sonia
Álvarez-Tabarés, Isabel
Weber, Isabella
Steinberg, Gero
Pérez-Martín, José
author_role author
author2 Álvarez-Tabarés, Isabel
Weber, Isabella
Steinberg, Gero
Pérez-Martín, José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cdk5
Corn smut
Cell polarity
Morphogenesis
Ustilago maydis
topic Cdk5
Corn smut
Cell polarity
Morphogenesis
Ustilago maydis
description Cyclin-dependent kinases from the Cdk5/Pho85 family are thought to play important roles in morphogenesis in organisms as diverse as yeast and humans. Here we used the corn smut fungus Ustilago maydis to address the role of Cdk5/Pho85 kinases in the morphogenesis and virulence of dimorphic phytopathogens. We found that Cdk5 is essential for growth in U. maydis. A temperature-sensitive cdk5 mutant caused cell wall and morphology defects at the restrictive temperature. Actin patches labeled with a fimbrin-GFP fusion protein were delocalized and a GFP-Myo5 fusion was directed towards the growing cell pole and rapidly dissociated from the tip. These defects were found to be due to an impairment in the maintenance of cell polarity. Our results indicated that Cdk5 is required for the activity of Rac1, probably at the level of the localization of its GEF, Cdc24. Cdk5 was required for full virulence, probably because mutant cells are unable to sustain the dramatic polar growth required for the formation of the infective structures. These results support a major role for morphogenesis in the virulence program of dimorphic fungi.
publishDate 2007
dc.date.none.fl_str_mv 2007
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/339636
url http://hdl.handle.net/10261/339636
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jcs.005314
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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