Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum

A Lactobacillus plantarum strain, CECT 4645, was found to have insertions of a sequence (985 bp in length) at least in eight loci in its genome. The prototype copy (Lp1) of this insertion sequence (named ISLpl4) has one open reading frame encoding a putative protein that is 292 amino acids in length...

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Detalhes bibliográficos
Autores: De Las Rivas, Blanca, Marcobal, Ángela, Gómez, Alicia, Muñoz, Rosario
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2005
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/44861
Acesso em linha:http://hdl.handle.net/10261/44861
Access Level:acceso abierto
Palavra-chave:Lactic acid bacteria
Transposase
Frameshift
Mobile elements
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spelling Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarumDe Las Rivas, BlancaMarcobal, ÁngelaGómez, AliciaMuñoz, RosarioLactic acid bacteriaTransposaseFrameshiftMobile elementsA Lactobacillus plantarum strain, CECT 4645, was found to have insertions of a sequence (985 bp in length) at least in eight loci in its genome. The prototype copy (Lp1) of this insertion sequence (named ISLpl4) has one open reading frame encoding a putative protein that is 292 amino acids in length with significant levels of similarity with IS982 family transposases. Perfect 16-bp inverted repeats were found at its termini. Upon transposition, generates 8-bp direct repeats of the target sequence, but no consensus sequences could be identified at either insertion site. The ISLpl4 pattern changed over many generations on the CECT 4645 strain. This finding strongly supports our hypothesis that ISLpl4 is a functional element in L. plantarum. Some of these elements may be cryptic, since point mutation or 1-nucleotide deletions were found in their transposase encoding genes. ISLpl4 copies have been detected in Leuconostoc mesenteroides, Oenococcus oeni, and Lactobacillus sakei. An ISLpl4 copy of O. oeni contained a +1 nucleotide insertion on its transposase encoding gene and, by using an experimental system, we were able to demonstrate that this specific sequence originates a +1 programmed translational frameshifting. Although the frameshifting process reported here operates at a low rate, this description might represent the first case of a functional +1 frameshifting among ISThis work was supported by grant RM03-002 from the INIA. We thank E. García, R. González and A.V. Carrascosa for their critical reading of the manuscript. The technical assistance of M.V. Santamaría is greatly appreciated. B. de las Rivas was a recipient of a postdoctoral fellowship and A. Marcobal of a predoctoral fellowship both from the Comunidad de Madrid. The DNA sequences of the ISLpl4 copies have been deposited in the GeneBank database under accession no. AJ968657 to AJ968664, and AJ781303. ISLpl4 was named according to the IS database curators (http://www-is.biotoul.fr).Peer reviewedElsevierCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220122005info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/44861reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.gene.2005.09.005Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/448612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
title Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
spellingShingle Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
De Las Rivas, Blanca
Lactic acid bacteria
Transposase
Frameshift
Mobile elements
title_short Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
title_full Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
title_fullStr Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
title_full_unstemmed Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
title_sort Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum
dc.creator.none.fl_str_mv De Las Rivas, Blanca
Marcobal, Ángela
Gómez, Alicia
Muñoz, Rosario
author De Las Rivas, Blanca
author_facet De Las Rivas, Blanca
Marcobal, Ángela
Gómez, Alicia
Muñoz, Rosario
author_role author
author2 Marcobal, Ángela
Gómez, Alicia
Muñoz, Rosario
author2_role author
author
author
dc.contributor.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lactic acid bacteria
Transposase
Frameshift
Mobile elements
topic Lactic acid bacteria
Transposase
Frameshift
Mobile elements
description A Lactobacillus plantarum strain, CECT 4645, was found to have insertions of a sequence (985 bp in length) at least in eight loci in its genome. The prototype copy (Lp1) of this insertion sequence (named ISLpl4) has one open reading frame encoding a putative protein that is 292 amino acids in length with significant levels of similarity with IS982 family transposases. Perfect 16-bp inverted repeats were found at its termini. Upon transposition, generates 8-bp direct repeats of the target sequence, but no consensus sequences could be identified at either insertion site. The ISLpl4 pattern changed over many generations on the CECT 4645 strain. This finding strongly supports our hypothesis that ISLpl4 is a functional element in L. plantarum. Some of these elements may be cryptic, since point mutation or 1-nucleotide deletions were found in their transposase encoding genes. ISLpl4 copies have been detected in Leuconostoc mesenteroides, Oenococcus oeni, and Lactobacillus sakei. An ISLpl4 copy of O. oeni contained a +1 nucleotide insertion on its transposase encoding gene and, by using an experimental system, we were able to demonstrate that this specific sequence originates a +1 programmed translational frameshifting. Although the frameshifting process reported here operates at a low rate, this description might represent the first case of a functional +1 frameshifting among IS
publishDate 2005
dc.date.none.fl_str_mv 2005
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/44861
url http://hdl.handle.net/10261/44861
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.gene.2005.09.005
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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