Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture

Aquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lact...

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Autores: Contente, Diogo, Díaz Formoso, Lara, Feito Hermida, Javier, Hernández Cruza, Pablo Elpidio, Muñoz Atienza, Estefanía, Borrero Del Pino, Juan, Poeta, Patrícia, Cintas Izarra, Luis Miguel
Tipo de documento: artigo
Data de publicação:2024
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/103836
Acesso em linha:https://hdl.handle.net/20.500.14352/103836
Access Level:Acceso aberto
Palavra-chave:612.39
Aquaculture
Larviculture
Rotifers (Brachionus plicatilis)
Probiotics
Lactic acid bacteria
Lactobacilli
WGS
Bioinformatics
Plantaricins
Ciencias Biomédicas
3104.06 Nutrición
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repository_id_str
spelling Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for AquacultureContente, DiogoDíaz Formoso, LaraFeito Hermida, JavierHernández Cruza, Pablo ElpidioMuñoz Atienza, EstefaníaBorrero Del Pino, JuanPoeta, PatríciaCintas Izarra, Luis Miguel612.39AquacultureLarvicultureRotifers (Brachionus plicatilis)ProbioticsLactic acid bacteriaLactobacilliWGSBioinformaticsPlantaricinsCiencias Biomédicas3104.06 NutriciónAquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lacticaseibacillus paracasei (BF3 and RT4) and two Lactiplantibacillus plantarum (BF12 and WT12) strains isolated from a rotifer cultivation tank used for turbot larviculture. Whole-genome sequencing (WGS) and bioinformatics analyses confirmed their probiotic potential, the absence of transferable antibiotic resistance genes, and the absence of virulence and pathogenicity factors. Bacteriocin mining identified a gene cluster encoding six plantaricins, suggesting their role in the antimicrobial activity exerted by these strains. In vitro cell-free protein synthesis (IV-CFPS) analyses was used to evaluate the expression of the plantaricin genes. The in vitro-synthesized class IIb (two-peptide bacteriocins) plantaricin E/F (PlnE/F) exerted antimicrobial activity against three indicator microorganisms, including the well-known ichthyopathogen Lactococcus garvieae. Furthermore, MALDI-TOF MS on colonies detected the presence of a major peptide that matches the dimeric form of plantaricins E (PlnE) and F (PlnF). This study emphasizes the importance of genome sequencing and bioinformatic analysis for evaluating aquaculture probiotic candidates. Moreover, it provides valuable insights into their genetic features and antimicrobial mechanisms, paving the way for their application as probiotics in larviculture, which is a major bottleneck in aquaculture.MDPIFigueras, AntonioUniversidad Complutense de Madrid20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103836reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMCIU RTI2018 094907-B-I00MCIU PID2019 104808RA-I00open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1038362026-06-02T12:44:21Z
dc.title.none.fl_str_mv Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
title Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
spellingShingle Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
Contente, Diogo
612.39
Aquaculture
Larviculture
Rotifers (Brachionus plicatilis)
Probiotics
Lactic acid bacteria
Lactobacilli
WGS
Bioinformatics
Plantaricins
Ciencias Biomédicas
3104.06 Nutrición
title_short Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
title_full Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
title_fullStr Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
title_full_unstemmed Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
title_sort Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
dc.creator.none.fl_str_mv Contente, Diogo
Díaz Formoso, Lara
Feito Hermida, Javier
Hernández Cruza, Pablo Elpidio
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Poeta, Patrícia
Cintas Izarra, Luis Miguel
author Contente, Diogo
author_facet Contente, Diogo
Díaz Formoso, Lara
Feito Hermida, Javier
Hernández Cruza, Pablo Elpidio
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Poeta, Patrícia
Cintas Izarra, Luis Miguel
author_role author
author2 Díaz Formoso, Lara
Feito Hermida, Javier
Hernández Cruza, Pablo Elpidio
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Poeta, Patrícia
Cintas Izarra, Luis Miguel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Figueras, Antonio
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 612.39
Aquaculture
Larviculture
Rotifers (Brachionus plicatilis)
Probiotics
Lactic acid bacteria
Lactobacilli
WGS
Bioinformatics
Plantaricins
Ciencias Biomédicas
3104.06 Nutrición
topic 612.39
Aquaculture
Larviculture
Rotifers (Brachionus plicatilis)
Probiotics
Lactic acid bacteria
Lactobacilli
WGS
Bioinformatics
Plantaricins
Ciencias Biomédicas
3104.06 Nutrición
description Aquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lacticaseibacillus paracasei (BF3 and RT4) and two Lactiplantibacillus plantarum (BF12 and WT12) strains isolated from a rotifer cultivation tank used for turbot larviculture. Whole-genome sequencing (WGS) and bioinformatics analyses confirmed their probiotic potential, the absence of transferable antibiotic resistance genes, and the absence of virulence and pathogenicity factors. Bacteriocin mining identified a gene cluster encoding six plantaricins, suggesting their role in the antimicrobial activity exerted by these strains. In vitro cell-free protein synthesis (IV-CFPS) analyses was used to evaluate the expression of the plantaricin genes. The in vitro-synthesized class IIb (two-peptide bacteriocins) plantaricin E/F (PlnE/F) exerted antimicrobial activity against three indicator microorganisms, including the well-known ichthyopathogen Lactococcus garvieae. Furthermore, MALDI-TOF MS on colonies detected the presence of a major peptide that matches the dimeric form of plantaricins E (PlnE) and F (PlnF). This study emphasizes the importance of genome sequencing and bioinformatic analysis for evaluating aquaculture probiotic candidates. Moreover, it provides valuable insights into their genetic features and antimicrobial mechanisms, paving the way for their application as probiotics in larviculture, which is a major bottleneck in aquaculture.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103836
url https://hdl.handle.net/20.500.14352/103836
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MCIU RTI2018 094907-B-I00
MCIU PID2019 104808RA-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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