Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir

Myxoma (MYXV) and rabbit hemorrhagic disease (RHDV) viruses are pathogens of economic relevance for cuniculture and conservation concern for wild European rabbits (Oryctolagus cuniculus), recently classified as ‘Endangered’ in its native range. Large-scale serological surveys, facilitated by sample...

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Authors: Ferreira e Silva, Joana, Jiménez Ruiz, Saúl, Rodrigues, Marisa, Santos, Emídio, Castro Scholten, Sabrina, Lizana, Vitor, Martí Marco, Alba, Almeida, Tereza, Lopes, Ana M., Abrantes, Joana, Rouco Zufiaurre, Carlos, Santos, Nuno
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/171860
Online Access:https://hdl.handle.net/11441/171860
https://doi.org/10.1016/j.prevetmed.2024.106369
Access Level:Open access
Keyword:Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
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spelling Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild ReservoirFerreira e Silva, JoanaJiménez Ruiz, SaúlRodrigues, MarisaSantos, EmídioCastro Scholten, SabrinaLizana, VitorMartí Marco, AlbaAlmeida, TerezaLopes, Ana M.Abrantes, JoanaRouco Zufiaurre, CarlosSantos, NunoEpidemiologyEuropean rabbitOryctolagus cuniculusProtein Saver cardsWildlifeMyxoma (MYXV) and rabbit hemorrhagic disease (RHDV) viruses are pathogens of economic relevance for cuniculture and conservation concern for wild European rabbits (Oryctolagus cuniculus), recently classified as ‘Endangered’ in its native range. Large-scale serological surveys, facilitated by sample collection using dried blood spots (DBS), allow monitoring seroprevalence in the wild reservoir but require evaluating the technique for the host and pathogen of interest. This study aimed to evaluate Protein Saver 903 DBS for MYXV and RHDV (genotype GI.2) serological surveys in European rabbits. Paired serum and DBS collected from 172 rabbits harvested or found dead in the Iberian Peninsula were tested for IgG antibodies specific against MYXV and RHDV GI.2 using indirect ELISA. We found an almost perfect agreement between serum and DBS for MYXV (Cohen's κ=0.914, CI95 0.847 – 0.981) and a strong agreement for RHDV GI.2 (Cohen's κ=0.808, CI95=0.722 – 0.893). The diagnostic sensitivity of DBS was 95.4 % (CI95 90.3 – 97.9 %) for MYXV and 82.1 % (CI95 73.2 – 88.5 %) for RHDV GI.2. The diagnostic specificity and positive predictive value were 100 % for both pathogens. This study supports DBS as a suitable sampling strategy for serological surveys of antibodies specific to MYXV and RHDV GI.2 in European rabbits, which generally agrees with results from other hosts and pathogens where this technique was evaluated.ElsevierBiología Vegetal y EcologíaFundação para a Ciência e a Tecnologia. PortugalEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)Ministerio de Ciencia, Innovación y Universidades (MICIU). EspañaMinisterio de Ciencia e Innovación (MICIN). EspañaLife Iberconejo2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/171860https://doi.org/10.1016/j.prevetmed.2024.106369reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPreventive Veterinary Medicine, 234, 106369.SFRH/BPD/116596/2016CEECIND/01388/2017CEECIND/00078/2017PRIMA/0003/2018NORTE-01–0246-FEDER-000063PRIMA S2–11-PCI2019–103732FPU19/06026PID2023–151954NB-100PID2019–111080RB-C21JDC2022–048850-ILIFE20-GIE ES000731https://doi.org/10.1016/j.prevetmed.2024.106369info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1718602026-06-17T12:51:07Z
dc.title.none.fl_str_mv Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
title Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
spellingShingle Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
Ferreira e Silva, Joana
Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
title_short Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
title_full Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
title_fullStr Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
title_full_unstemmed Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
title_sort Evaluation of Dried Blood Spots for Serological Surveys of Myxoma and Rabbit Hemorrhagic Disease Viruses in their Wild Reservoir
dc.creator.none.fl_str_mv Ferreira e Silva, Joana
Jiménez Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro Scholten, Sabrina
Lizana, Vitor
Martí Marco, Alba
Almeida, Tereza
Lopes, Ana M.
Abrantes, Joana
Rouco Zufiaurre, Carlos
Santos, Nuno
author Ferreira e Silva, Joana
author_facet Ferreira e Silva, Joana
Jiménez Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro Scholten, Sabrina
Lizana, Vitor
Martí Marco, Alba
Almeida, Tereza
Lopes, Ana M.
Abrantes, Joana
Rouco Zufiaurre, Carlos
Santos, Nuno
author_role author
author2 Jiménez Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro Scholten, Sabrina
Lizana, Vitor
Martí Marco, Alba
Almeida, Tereza
Lopes, Ana M.
Abrantes, Joana
Rouco Zufiaurre, Carlos
Santos, Nuno
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Vegetal y Ecología
Fundação para a Ciência e a Tecnologia. Portugal
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Ministerio de Ciencia, Innovación y Universidades (MICIU). España
Ministerio de Ciencia e Innovación (MICIN). España
Life Iberconejo
dc.subject.none.fl_str_mv Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
topic Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
description Myxoma (MYXV) and rabbit hemorrhagic disease (RHDV) viruses are pathogens of economic relevance for cuniculture and conservation concern for wild European rabbits (Oryctolagus cuniculus), recently classified as ‘Endangered’ in its native range. Large-scale serological surveys, facilitated by sample collection using dried blood spots (DBS), allow monitoring seroprevalence in the wild reservoir but require evaluating the technique for the host and pathogen of interest. This study aimed to evaluate Protein Saver 903 DBS for MYXV and RHDV (genotype GI.2) serological surveys in European rabbits. Paired serum and DBS collected from 172 rabbits harvested or found dead in the Iberian Peninsula were tested for IgG antibodies specific against MYXV and RHDV GI.2 using indirect ELISA. We found an almost perfect agreement between serum and DBS for MYXV (Cohen's κ=0.914, CI95 0.847 – 0.981) and a strong agreement for RHDV GI.2 (Cohen's κ=0.808, CI95=0.722 – 0.893). The diagnostic sensitivity of DBS was 95.4 % (CI95 90.3 – 97.9 %) for MYXV and 82.1 % (CI95 73.2 – 88.5 %) for RHDV GI.2. The diagnostic specificity and positive predictive value were 100 % for both pathogens. This study supports DBS as a suitable sampling strategy for serological surveys of antibodies specific to MYXV and RHDV GI.2 in European rabbits, which generally agrees with results from other hosts and pathogens where this technique was evaluated.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/171860
https://doi.org/10.1016/j.prevetmed.2024.106369
url https://hdl.handle.net/11441/171860
https://doi.org/10.1016/j.prevetmed.2024.106369
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Preventive Veterinary Medicine, 234, 106369.
SFRH/BPD/116596/2016
CEECIND/01388/2017
CEECIND/00078/2017
PRIMA/0003/2018
NORTE-01–0246-FEDER-000063
PRIMA S2–11-PCI2019–103732
FPU19/06026
PID2023–151954NB-100
PID2019–111080RB-C21
JDC2022–048850-I
LIFE20-GIE ES000731
https://doi.org/10.1016/j.prevetmed.2024.106369
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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