3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease

The entorhinal cortex (EC) is especially vulnerable in the early stages of Alzheimer's disease (AD). In particular, cognitive deficits have been linked to alterations in the upper layers of EC. In the present report, we examined Layers II and III from eight human brain autopsies (four subjects...

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Autores: Domínguez-Álvaro, M., Montero-Crespo, M., Blázquez-Llorca, Lidia, Plaza-Alonso, Sergio, Cano-Astorga, Nicolás, DeFelipe, Javier, Alonso-Nanclares, Lidia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/249302
Acceso en línea:http://hdl.handle.net/10261/249302
Access Level:acceso abierto
Palabra clave:dementia
Electron microscopy
FIB/SEM
Medial temporal lobe
neuropil
postsynaptic targets
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spelling 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's DiseaseDomínguez-Álvaro, M.Montero-Crespo, M.Blázquez-Llorca, LidiaPlaza-Alonso, SergioCano-Astorga, NicolásDeFelipe, JavierAlonso-Nanclares, LidiadementiaElectron microscopyFIB/SEMMedial temporal lobeneuropilpostsynaptic targetsThe entorhinal cortex (EC) is especially vulnerable in the early stages of Alzheimer's disease (AD). In particular, cognitive deficits have been linked to alterations in the upper layers of EC. In the present report, we examined Layers II and III from eight human brain autopsies (four subjects with no recorded neurologic alterations and four AD cases). We used stereological methods to assess cortical atrophy of the EC and possible changes in the volume occupied by different cortical elements (neuronal and glial cell bodies; blood vessels; and neuropil). We performed 3D ultrastructural analyses of synapses using focused ion beam/scanning electron microscopy (FIB/SEM) to examine possible alterations related to AD. At the light microscope level, we found a significantly lower volume fraction occupied by neuronal bodies in Layer III and a higher volume fraction occupied by glial cell bodies in Layer II in AD cases. At the ultrastructural level, we observed that (1) there was a significantly lower synaptic density in both layers in AD cases; (2) synapses were larger and more complex in Layer II in AD cases; and (3) there was a greater proportion of small and simple synapses in Layer III in AD cases than in control individuals. These structural differences may play a role in the anatomic basis for the impairment of cognitive functions in AD.Society for NeuroscienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/249302reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1523/ENEURO.0504-20.2021Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2493022026-05-22T06:33:51Z
dc.title.none.fl_str_mv 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
title 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
spellingShingle 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
Domínguez-Álvaro, M.
dementia
Electron microscopy
FIB/SEM
Medial temporal lobe
neuropil
postsynaptic targets
title_short 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
title_full 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
title_fullStr 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
title_full_unstemmed 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
title_sort 3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease
dc.creator.none.fl_str_mv Domínguez-Álvaro, M.
Montero-Crespo, M.
Blázquez-Llorca, Lidia
Plaza-Alonso, Sergio
Cano-Astorga, Nicolás
DeFelipe, Javier
Alonso-Nanclares, Lidia
author Domínguez-Álvaro, M.
author_facet Domínguez-Álvaro, M.
Montero-Crespo, M.
Blázquez-Llorca, Lidia
Plaza-Alonso, Sergio
Cano-Astorga, Nicolás
DeFelipe, Javier
Alonso-Nanclares, Lidia
author_role author
author2 Montero-Crespo, M.
Blázquez-Llorca, Lidia
Plaza-Alonso, Sergio
Cano-Astorga, Nicolás
DeFelipe, Javier
Alonso-Nanclares, Lidia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv dementia
Electron microscopy
FIB/SEM
Medial temporal lobe
neuropil
postsynaptic targets
topic dementia
Electron microscopy
FIB/SEM
Medial temporal lobe
neuropil
postsynaptic targets
description The entorhinal cortex (EC) is especially vulnerable in the early stages of Alzheimer's disease (AD). In particular, cognitive deficits have been linked to alterations in the upper layers of EC. In the present report, we examined Layers II and III from eight human brain autopsies (four subjects with no recorded neurologic alterations and four AD cases). We used stereological methods to assess cortical atrophy of the EC and possible changes in the volume occupied by different cortical elements (neuronal and glial cell bodies; blood vessels; and neuropil). We performed 3D ultrastructural analyses of synapses using focused ion beam/scanning electron microscopy (FIB/SEM) to examine possible alterations related to AD. At the light microscope level, we found a significantly lower volume fraction occupied by neuronal bodies in Layer III and a higher volume fraction occupied by glial cell bodies in Layer II in AD cases. At the ultrastructural level, we observed that (1) there was a significantly lower synaptic density in both layers in AD cases; (2) synapses were larger and more complex in Layer II in AD cases; and (3) there was a greater proportion of small and simple synapses in Layer III in AD cases than in control individuals. These structural differences may play a role in the anatomic basis for the impairment of cognitive functions in AD.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/249302
url http://hdl.handle.net/10261/249302
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1523/ENEURO.0504-20.2021
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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