Enzyme inactivation and changes in the properties of cloudy apple juice after high‐pressure carbon dioxide and thermosonication treatments and during refrigerated storage

High-pressure carbon dioxide and thermosonication technologies were applied to cloudy apple juice to determine their effect on polyphenol oxidase (PPO) and pectin methylesterase (PME) activity and other quality parameters right after the treatment and during storage at 4ºC for 28 days. Treatment con...

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Detalhes bibliográficos
Autores: Illera Gigante, Alba Ester, Beltrán Calvo, Sagrario, Sanz Díez, Mª Teresa
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2020
País:España
Recursos:Universidad de Burgos (UBU)
Repositório:Repositorio Institucional de la Universidad de Burgos (RIUBU)
OAI Identifier:oai:riubu.ubu.es:10259/11349
Acesso em linha:https://hdl.handle.net/10259/11349
Access Level:Acceso aberto
Palavra-chave:Cloudy apple juice
Non-thermal treatment
Enzymatic inactivation
Quality parameters
Refrigerated storage
Tecnología de los alimentos
Alimentos-Conservación
Food-Preservation
Descrição
Resumo:High-pressure carbon dioxide and thermosonication technologies were applied to cloudy apple juice to determine their effect on polyphenol oxidase (PPO) and pectin methylesterase (PME) activity and other quality parameters right after the treatment and during storage at 4ºC for 28 days. Treatment conditions were 20 MPa, 45ºC, for 60 min and 20 kHz, 62ºC, 100% amplitude for 20 min, for pressurized CO2 and thermosonication treatments, respectively. Both treatments showed a great impact on PPO activity and its residual activity steadily decreased during storage at 4ºC. In contrast, PME was found to be more resistant to the treatments. After both treatments, a homogenization effect was observed, which was also reflected in an increase in the cloud value of the juices after the treatments, kept during storage. Antioxidant capacity and total polyphenol content kept high levels during storage after both treatments.