Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord

Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens...

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Autores: Paniagua-González, Lucía, Jiménez Morigosa, Cristian, Lendoiro Belío, Elena, Concheiro, Marta, Cruz Landeira, Angelines, López-Rivadulla Lamas, Manuel, Castro Ríos, Ana de
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/39115
Acceso en línea:https://hdl.handle.net/10347/39115
Access Level:acceso abierto
Palabra clave:LC-MS/MS
Nicotine
Placenta
Tobacco
Umbilical cord
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spelling Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cordPaniagua-González, LucíaJiménez Morigosa, CristianLendoiro Belío, ElenaConcheiro, MartaCruz Landeira, AngelinesLópez-Rivadulla Lamas, ManuelCastro Ríos, Ana deLC-MS/MSNicotinePlacentaTobaccoUmbilical cordTobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis® HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2 ng/g for cotinine and OH-cotinine, and 5 ng/g for nicotine) and quantification (5 ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (-34.1 to -84.5% in PL, %CV = 9.1-24.0%; -18.9 to -84.7% in UC, %CV = 10.2-23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72 h in the autosampler (<11.5% loss in PL, and < 13% loss in UC). The method was applied to 14 PL and UC specimens from tobacco users during pregnancy. Cotinine (6.8-312.2 ng/g in PL; 6.7-342.3 ng/g in UC) was the predominant analyte, followed by OH-cotinine (<LOQ-80.2 ng/g in PL; <LOQ-80.5 ng/g in UC) and nicotine (5.7-63.7 ng/g in PL; 5.1-63.3 ng/g in UC). This method will be applied to more than 150 specimens collected from a wide clinical study to evaluate the usefulness of maternal hair, meconium, placenta and umbilical cord compared to the maternal interview to detect in utero drug exposure.WileyUniversidade de Santiago de Compostela. Departamento de Anatomía Patolóxica e Ciencias Forenses20182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/39115reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/391152026-06-15T12:47:27Z
dc.title.none.fl_str_mv Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
title Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
spellingShingle Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
Paniagua-González, Lucía
LC-MS/MS
Nicotine
Placenta
Tobacco
Umbilical cord
title_short Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
title_full Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
title_fullStr Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
title_full_unstemmed Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
title_sort Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
dc.creator.none.fl_str_mv Paniagua-González, Lucía
Jiménez Morigosa, Cristian
Lendoiro Belío, Elena
Concheiro, Marta
Cruz Landeira, Angelines
López-Rivadulla Lamas, Manuel
Castro Ríos, Ana de
author Paniagua-González, Lucía
author_facet Paniagua-González, Lucía
Jiménez Morigosa, Cristian
Lendoiro Belío, Elena
Concheiro, Marta
Cruz Landeira, Angelines
López-Rivadulla Lamas, Manuel
Castro Ríos, Ana de
author_role author
author2 Jiménez Morigosa, Cristian
Lendoiro Belío, Elena
Concheiro, Marta
Cruz Landeira, Angelines
López-Rivadulla Lamas, Manuel
Castro Ríos, Ana de
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Anatomía Patolóxica e Ciencias Forenses
dc.subject.none.fl_str_mv LC-MS/MS
Nicotine
Placenta
Tobacco
Umbilical cord
topic LC-MS/MS
Nicotine
Placenta
Tobacco
Umbilical cord
description Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis® HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2 ng/g for cotinine and OH-cotinine, and 5 ng/g for nicotine) and quantification (5 ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (-34.1 to -84.5% in PL, %CV = 9.1-24.0%; -18.9 to -84.7% in UC, %CV = 10.2-23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72 h in the autosampler (<11.5% loss in PL, and < 13% loss in UC). The method was applied to 14 PL and UC specimens from tobacco users during pregnancy. Cotinine (6.8-312.2 ng/g in PL; 6.7-342.3 ng/g in UC) was the predominant analyte, followed by OH-cotinine (<LOQ-80.2 ng/g in PL; <LOQ-80.5 ng/g in UC) and nicotine (5.7-63.7 ng/g in PL; 5.1-63.3 ng/g in UC). This method will be applied to more than 150 specimens collected from a wide clinical study to evaluate the usefulness of maternal hair, meconium, placenta and umbilical cord compared to the maternal interview to detect in utero drug exposure.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/39115
url https://hdl.handle.net/10347/39115
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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