Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures

19 páginas, 4 tablas, 3 figuras.

Detalles Bibliográficos
Autores: Vargas, J.E., López-Ferreras, L., Andrés, Sonia, Mateos, I., Horst, E.H., López, Secundino
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/311192
Acceso en línea:http://hdl.handle.net/10261/311192
Access Level:acceso abierto
Palabra clave:Rumen
RUSITEC
In vitro
Continuous cultures
Forage
Concentrate
Buffer
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spelling Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous culturesVargas, J.E.López-Ferreras, L.Andrés, SoniaMateos, I.Horst, E.H.López, SecundinoRumenRUSITECIn vitroContinuous culturesForageConcentrateBuffer19 páginas, 4 tablas, 3 figuras.The aim of this study was to distinguish effects due to diet composition from those triggered by ruminal pH on fermentation patterns and microbial profiles in a continuous culture system (RUSITEC). The study followed a 2 × 2 factorial design, with two diets varying in the proportions of forage and concentrate and two pH levels in the culture medium. RUSITEC fermenters were used to simulate rumen fermentation and feed digestibility, fermentation end-products, microbial protein synthesis, microbial community, and long-chain fatty acid profiles in the digesta were determined. Multivariate analyses were applied to summarize the overall results. High concentrate (34% cereal grain, 32% hay) diets were more digestible (p < 0.05) than high forage (10% cereal grain, 78% hay) diets, resulting in a greater (p < 0.05) formation of most fermentation end-products and microbial protein in the rumen. However, there were no significant (p > 0.05) differences between diets in methane production. Ciliate protozoa, anaerobic fungi, some fibrolytic bacteria, hydrogenation of oleic acid, and relative proportion of conjugated linoleic acid were increased (p < 0.05) with high forage diets. A decline in rumen pH from 6.8 to 6.4 decreased (p < 0.05) feed digestibility, protein degradability, and the daily outputs of some fermentation end-products (gas, VFA, acetate, ammonia) but had no effect (p > 0.05) on the synthesis of microbial protein, and on the output of methane, propionate, butyrate or lactate. Minor changes in microbial community profile or the fatty acid relative proportions were observed within this pH range. The overall multivariate analysis revealed a clear discrimination between high-concentrate and high-forage diets, with subtler and less-defined pH effects on ruminal fermentation and microbial communities.This work was funded by the Spanish Ministerio de Ciencia e Innovación (PID2021- 126489OB-I00, MCIN/AEI/10.13039/501100011033) and co-funded by the European Regional Development Fund (ERDF-FEDER) ”A way to make Europe-Una manera de hacer Europa”). Funding for the payment of APC was provided by Universidad de León (Ayudas a Grupos de Investigación BB202).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)European CommissionUniversidad de LeónLópez, Secundino [0000-0001-6267-683X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/311192reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126489OB-I00http://dx.doi.org/10.3390/fermentation9040320Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3111922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
title Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
spellingShingle Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
Vargas, J.E.
Rumen
RUSITEC
In vitro
Continuous cultures
Forage
Concentrate
Buffer
title_short Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
title_full Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
title_fullStr Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
title_full_unstemmed Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
title_sort Differential diet and pH effects on ruminal mibrobiota, fermentation pattern and fatty acid hydrogenation in RUSITEC continuous cultures
dc.creator.none.fl_str_mv Vargas, J.E.
López-Ferreras, L.
Andrés, Sonia
Mateos, I.
Horst, E.H.
López, Secundino
author Vargas, J.E.
author_facet Vargas, J.E.
López-Ferreras, L.
Andrés, Sonia
Mateos, I.
Horst, E.H.
López, Secundino
author_role author
author2 López-Ferreras, L.
Andrés, Sonia
Mateos, I.
Horst, E.H.
López, Secundino
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Universidad de León
López, Secundino [0000-0001-6267-683X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Rumen
RUSITEC
In vitro
Continuous cultures
Forage
Concentrate
Buffer
topic Rumen
RUSITEC
In vitro
Continuous cultures
Forage
Concentrate
Buffer
description 19 páginas, 4 tablas, 3 figuras.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/311192
url http://hdl.handle.net/10261/311192
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126489OB-I00
http://dx.doi.org/10.3390/fermentation9040320
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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