Sperm head phenotype and male fertility in ram semen

Although there is ample evidence for the effects of sperm head shape on sperm function, its impact on fertility has not been explored in detail at the intraspecific level in mammals. Here, we assess the relationship between sperm head shape and male fertility in a large-scale study in Manchega sheep...

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Autores: Maroto-Morales, Alejandro, Ramón, Manuel, García-Álvarez, Olga, Montoro, Vidal, Soler, Ana J., Fernández-Santos, M. Rocío, Roldán, Eduardo R. S., Pérez-Guzmán, M. D., Garde, José Julián
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::62999fda510089ec4658d0f7cfc40f85
Acceso en línea:http://hdl.handle.net/10261/128354
Access Level:acceso abierto
Palabra clave:Fertility
Ejaculate heterogeneity
Sperm head shape
Sperm morphometry
Sheep
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spelling Sperm head phenotype and male fertility in ram semenMaroto-Morales, AlejandroRamón, ManuelGarcía-Álvarez, OlgaMontoro, VidalSoler, Ana J.Fernández-Santos, M. RocíoRoldán, Eduardo R. S.Pérez-Guzmán, M. D.Garde, José JuliánFertilityEjaculate heterogeneitySperm head shapeSperm morphometrySheepAlthough there is ample evidence for the effects of sperm head shape on sperm function, its impact on fertility has not been explored in detail at the intraspecific level in mammals. Here, we assess the relationship between sperm head shape and male fertility in a large-scale study in Manchega sheep (Ovis aries), which have not undergone any selection for fertility. Semen was collected from 83 mature rams, and before insemination, head shapes were measured for five parameters: area, perimeter, length, width, and p2a (perimeter2/2×π×area) using a computer-assisted sperm morphometric analysis. In addition, a cluster analysis using sperm head length and p2a factor was performed to determine sperm subpopulations (SPs) structure. Our results show the existence of four sperm SPs, which present different sperm head phenotype: SP1 (large and round), SP2 (short and elongated), SP3 (shortest and round), and SP4 (large and the most elongated). No relationships were found between males' fertility rates and average values of sperm head dimensions. However, differences in fertility rates between rams were strongly associated to the proportion of spermatozoa in an ejaculate SP with short and elongated heads (P < 0.001). These findings show how the heterogeneity in sperm head shape of the ejaculate has an effect on reproductive success, and highlight the important role of modulation of the ejaculate at the intraspecific level.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/128354reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.theriogenology.2015.07.038Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::62999fda510089ec4658d0f7cfc40f852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sperm head phenotype and male fertility in ram semen
title Sperm head phenotype and male fertility in ram semen
spellingShingle Sperm head phenotype and male fertility in ram semen
Maroto-Morales, Alejandro
Fertility
Ejaculate heterogeneity
Sperm head shape
Sperm morphometry
Sheep
title_short Sperm head phenotype and male fertility in ram semen
title_full Sperm head phenotype and male fertility in ram semen
title_fullStr Sperm head phenotype and male fertility in ram semen
title_full_unstemmed Sperm head phenotype and male fertility in ram semen
title_sort Sperm head phenotype and male fertility in ram semen
dc.creator.none.fl_str_mv Maroto-Morales, Alejandro
Ramón, Manuel
García-Álvarez, Olga
Montoro, Vidal
Soler, Ana J.
Fernández-Santos, M. Rocío
Roldán, Eduardo R. S.
Pérez-Guzmán, M. D.
Garde, José Julián
author Maroto-Morales, Alejandro
author_facet Maroto-Morales, Alejandro
Ramón, Manuel
García-Álvarez, Olga
Montoro, Vidal
Soler, Ana J.
Fernández-Santos, M. Rocío
Roldán, Eduardo R. S.
Pérez-Guzmán, M. D.
Garde, José Julián
author_role author
author2 Ramón, Manuel
García-Álvarez, Olga
Montoro, Vidal
Soler, Ana J.
Fernández-Santos, M. Rocío
Roldán, Eduardo R. S.
Pérez-Guzmán, M. D.
Garde, José Julián
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fertility
Ejaculate heterogeneity
Sperm head shape
Sperm morphometry
Sheep
topic Fertility
Ejaculate heterogeneity
Sperm head shape
Sperm morphometry
Sheep
description Although there is ample evidence for the effects of sperm head shape on sperm function, its impact on fertility has not been explored in detail at the intraspecific level in mammals. Here, we assess the relationship between sperm head shape and male fertility in a large-scale study in Manchega sheep (Ovis aries), which have not undergone any selection for fertility. Semen was collected from 83 mature rams, and before insemination, head shapes were measured for five parameters: area, perimeter, length, width, and p2a (perimeter2/2×π×area) using a computer-assisted sperm morphometric analysis. In addition, a cluster analysis using sperm head length and p2a factor was performed to determine sperm subpopulations (SPs) structure. Our results show the existence of four sperm SPs, which present different sperm head phenotype: SP1 (large and round), SP2 (short and elongated), SP3 (shortest and round), and SP4 (large and the most elongated). No relationships were found between males' fertility rates and average values of sperm head dimensions. However, differences in fertility rates between rams were strongly associated to the proportion of spermatozoa in an ejaculate SP with short and elongated heads (P < 0.001). These findings show how the heterogeneity in sperm head shape of the ejaculate has an effect on reproductive success, and highlight the important role of modulation of the ejaculate at the intraspecific level.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/128354
url http://hdl.handle.net/10261/128354
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2015.07.038
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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