Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS

Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable...

ver descrição completa

Detalhes bibliográficos
Autores: Romero Sánchez, Iván, Ramírez García, Lorena, Gracia Lor, Emma, Madrid Albarrán, María Yolanda
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/71776
Acesso em linha:https://hdl.handle.net/20.500.14352/71776
Access Level:acceso abierto
Palavra-chave:Aflatoxins
Cereals
Rice
Immunoaffinity columns
HPLC-MS/MS
Bioanalytical Chemistry
Química analítica (Química)
2301 Química Analítica
id ES_b7afd8b4268290bbfd02e9e626100cfd
oai_identifier_str oai:docta.ucm.es:20.500.14352/71776
network_acronym_str ES
network_name_str España
repository_id_str
spelling Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MSRomero Sánchez, IvánRamírez García, LorenaGracia Lor, EmmaMadrid Albarrán, María YolandaAflatoxinsCerealsRiceImmunoaffinity columnsHPLC-MS/MSBioanalytical ChemistryQuímica analítica (Química)2301 Química AnalíticaRice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol – water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86–92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals.ElsevierUniversidad Complutense de Madrid20222022-07-1120222022-07-11journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/71776reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/717762026-06-02T12:44:21Z
dc.title.none.fl_str_mv Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
title Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
spellingShingle Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
Romero Sánchez, Iván
Aflatoxins
Cereals
Rice
Immunoaffinity columns
HPLC-MS/MS
Bioanalytical Chemistry
Química analítica (Química)
2301 Química Analítica
title_short Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
title_full Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
title_fullStr Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
title_full_unstemmed Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
title_sort Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS
dc.creator.none.fl_str_mv Romero Sánchez, Iván
Ramírez García, Lorena
Gracia Lor, Emma
Madrid Albarrán, María Yolanda
author Romero Sánchez, Iván
author_facet Romero Sánchez, Iván
Ramírez García, Lorena
Gracia Lor, Emma
Madrid Albarrán, María Yolanda
author_role author
author2 Ramírez García, Lorena
Gracia Lor, Emma
Madrid Albarrán, María Yolanda
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Aflatoxins
Cereals
Rice
Immunoaffinity columns
HPLC-MS/MS
Bioanalytical Chemistry
Química analítica (Química)
2301 Química Analítica
topic Aflatoxins
Cereals
Rice
Immunoaffinity columns
HPLC-MS/MS
Bioanalytical Chemistry
Química analítica (Química)
2301 Química Analítica
description Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol – water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86–92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-11
2022
2022-07-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/71776
url https://hdl.handle.net/20.500.14352/71776
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417549453590528
score 15.301629

Registos relacionados