Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.

Chagas disease is a neglected tropical infection that affects over 6 million people worldwide. This study explores transcriptomic changes in <em>T. cruzi</em>-infected subjects before and after receiving treatment. Using total RNA sequencing, gene transcription was analyzed in peripheral...

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Autores: Ros Lucas, Albert, Alonso Padilla, Julio, Gabaldón Figueira, Juan Carlos, Martínez-Peinado, Nieves, Losada-Galván, I., Posada, Elizabeth, Escabia, Elisa, Martín Mur, Beatriz, Gut, Marta, Esteve Codina, Anna, Gascón, Joaquim, Pinazo Delgado, Mª Jesus
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Universidad de Oviedo (UNIOVI)
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/226484
Acceso en línea:https://hdl.handle.net/2445/226484
Access Level:acceso abierto
Palabra clave:Tripanosoma
Malaltia de Chagas
Immunitat
Trypanosoma
Chagas' disease
Immunity
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spelling Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.Ros Lucas, AlbertAlonso Padilla, JulioGabaldón Figueira, Juan CarlosMartínez-Peinado, NievesLosada-Galván, I.Posada, ElizabethEscabia, ElisaMartín Mur, BeatrizGut, MartaEsteve Codina, AnnaGascón, JoaquimPinazo Delgado, Mª JesusTripanosomaMalaltia de ChagasImmunitatTrypanosomaChagas' diseaseImmunityChagas disease is a neglected tropical infection that affects over 6 million people worldwide. This study explores transcriptomic changes in <em>T. cruzi</em>-infected subjects before and after receiving treatment. Using total RNA sequencing, gene transcription was analyzed in peripheral blood mononuclear cells from asymptomatic (n=19) and symptomatic (n=8) <em>T. cruzi</em>-infected individuals, and from non-infected controls (n=15). Differential expression was compared across groups and before/after treatment in <em>T. cruzi</em>-infected subgroups. Transcriptomic changes associated with untreated infection were observed in comparisons with controls, with 12 upregulated and 206 downregulated genes in all the subjects infected with <em>T. cruzi</em>, and 47 upregulated and 215 downregulated genes in the symptomatic group. Very few differentially expressed genes were found after treatment and between the different infected groups. A gene set enrichment analysis highlighted several immune-related pathways activated during the infection, with antiparasitic therapy normalizing immune function after treatment. This matched changes in the kynurenine/tryptophan ratio, increased in pre-treatment samples and suggesting chronic immune fatigue, which was restored following treatment. The described differentially expressed genes can provide insights for the study of new potential biomarkers and pathways associated with disease progression and treatment response.Oxford University Press2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/226484Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de Oviedo (UNIOVI)InglésVersió postprint del document publicat a: https://doi.org/10.1093/infdis/jiae429Journal of Infectious Diseases, 2024https://doi.org/10.1093/infdis/jiae429(c) Ros-Lucas, A. et al., 2024info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2264842026-05-27T06:46:51Z
dc.title.none.fl_str_mv Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
title Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
spellingShingle Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
Ros Lucas, Albert
Tripanosoma
Malaltia de Chagas
Immunitat
Trypanosoma
Chagas' disease
Immunity
title_short Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
title_full Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
title_fullStr Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
title_full_unstemmed Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
title_sort Transcriptomic changes and immune modulation associated with antiparasitic treatment in chronic Trypanosoma cruzi infection.
dc.creator.none.fl_str_mv Ros Lucas, Albert
Alonso Padilla, Julio
Gabaldón Figueira, Juan Carlos
Martínez-Peinado, Nieves
Losada-Galván, I.
Posada, Elizabeth
Escabia, Elisa
Martín Mur, Beatriz
Gut, Marta
Esteve Codina, Anna
Gascón, Joaquim
Pinazo Delgado, Mª Jesus
author Ros Lucas, Albert
author_facet Ros Lucas, Albert
Alonso Padilla, Julio
Gabaldón Figueira, Juan Carlos
Martínez-Peinado, Nieves
Losada-Galván, I.
Posada, Elizabeth
Escabia, Elisa
Martín Mur, Beatriz
Gut, Marta
Esteve Codina, Anna
Gascón, Joaquim
Pinazo Delgado, Mª Jesus
author_role author
author2 Alonso Padilla, Julio
Gabaldón Figueira, Juan Carlos
Martínez-Peinado, Nieves
Losada-Galván, I.
Posada, Elizabeth
Escabia, Elisa
Martín Mur, Beatriz
Gut, Marta
Esteve Codina, Anna
Gascón, Joaquim
Pinazo Delgado, Mª Jesus
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tripanosoma
Malaltia de Chagas
Immunitat
Trypanosoma
Chagas' disease
Immunity
topic Tripanosoma
Malaltia de Chagas
Immunitat
Trypanosoma
Chagas' disease
Immunity
description Chagas disease is a neglected tropical infection that affects over 6 million people worldwide. This study explores transcriptomic changes in <em>T. cruzi</em>-infected subjects before and after receiving treatment. Using total RNA sequencing, gene transcription was analyzed in peripheral blood mononuclear cells from asymptomatic (n=19) and symptomatic (n=8) <em>T. cruzi</em>-infected individuals, and from non-infected controls (n=15). Differential expression was compared across groups and before/after treatment in <em>T. cruzi</em>-infected subgroups. Transcriptomic changes associated with untreated infection were observed in comparisons with controls, with 12 upregulated and 206 downregulated genes in all the subjects infected with <em>T. cruzi</em>, and 47 upregulated and 215 downregulated genes in the symptomatic group. Very few differentially expressed genes were found after treatment and between the different infected groups. A gene set enrichment analysis highlighted several immune-related pathways activated during the infection, with antiparasitic therapy normalizing immune function after treatment. This matched changes in the kynurenine/tryptophan ratio, increased in pre-treatment samples and suggesting chronic immune fatigue, which was restored following treatment. The described differentially expressed genes can provide insights for the study of new potential biomarkers and pathways associated with disease progression and treatment response.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/226484
url https://hdl.handle.net/2445/226484
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1093/infdis/jiae429
Journal of Infectious Diseases, 2024
https://doi.org/10.1093/infdis/jiae429
dc.rights.none.fl_str_mv (c) Ros-Lucas, A. et al., 2024
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Ros-Lucas, A. et al., 2024
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Oviedo (UNIOVI)
instname_str Universidad de Oviedo (UNIOVI)
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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