Control of transcription by the stress activated Hog1 kinase
A fundamental property of living cells is the ability to sense and robustly respond to fluctuations in their environment. In budding yeast (Saccharomyces cerevisiae) changes in extracellular osmolarity are sensed by the HOG pathway, which evokes the program for cell adaptation required for cell surv...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/293257 |
| Acceso en línea: | http://hdl.handle.net/10803/293257 |
| Access Level: | acceso abierto |
| Palabra clave: | Gene expression Chromatin remodeling Hog1 MAPK Osmostress Tling arrays Expressió gènica Remodelació de cromatina Estrés osmòtic RNA no codificant 575 |
| Sumario: | A fundamental property of living cells is the ability to sense and robustly respond to fluctuations in their environment. In budding yeast (Saccharomyces cerevisiae) changes in extracellular osmolarity are sensed by the HOG pathway, which evokes the program for cell adaptation required for cell survival. The aim of this thesis was to further characterize the molecular mechanisms by which Hog1 regulates gene expression upon osmostress. A genome-wide genetic screen lead to the identification of several activities required for regulation of gene expression. Here we describe the characterization of a novel substrate for the SAPK whose activity is required for proper transcription initiation and elongation in response to stress. This thesis also aimed to globally characterize the role of Hog1 in reprogramming the transcriptome of S. cerevisiae under osmostress conditions. By the combination of molecular approaches coupled to genome-wide techniques (ChIP-seq, MNase-seq and Tiling arrays) we have been able to fully characterize the localization of the key components that drive osmoresponsive transcription, providing for the first time a complete picture of the transcription process. The high resolution of the genome-wide approaches, has allowed us to identify new transcriptional roles for the SAPK such as the targeting of RNA Pol III machinery, and the regulation of a novel class of functional long noncoding RNAs (lncRNA). In summary, results presented in this thesis provide novel insights into the mechanisms by which the Hog1 SAPK modulates gene expression. |
|---|