Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco

In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast...

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Autores: Ancín, María, Larraya, Luis, Florez-Sarasa, Igor, Bénard, Camille, Fernández-San Millán, Alicia, Veramendi, Jon, Gibon, Yves, Fernie, Alisdair R., Aranjuelo, Iker, Farran, Inmaculada
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1443
Acceso en línea:http://hdl.handle.net/20.500.12327/1443
https://doi.org/10.1093/jxb/erab193
Access Level:acceso abierto
Palabra clave:633
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spelling Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobaccoAncín, MaríaLarraya, LuisFlorez-Sarasa, IgorBénard, CamilleFernández-San Millán, AliciaVeramendi, JonGibon, YvesFernie, Alisdair R.Aranjuelo, IkerFarran, Inmaculada633In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.info:eu-repo/semantics/publishedVersionOxford University PressProducció VegetalGenòmica i Biotecnologia2021info:eu-repo/semantics/article16http://hdl.handle.net/20.500.12327/1443https://doi.org/10.1093/jxb/erab193reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésJournal of Experimental BotanyMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOMMICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biologyhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/14432026-05-29T05:05:01Z
dc.title.none.fl_str_mv Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
title Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
spellingShingle Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
Ancín, María
633
title_short Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
title_full Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
title_fullStr Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
title_full_unstemmed Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
title_sort Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
dc.creator.none.fl_str_mv Ancín, María
Larraya, Luis
Florez-Sarasa, Igor
Bénard, Camille
Fernández-San Millán, Alicia
Veramendi, Jon
Gibon, Yves
Fernie, Alisdair R.
Aranjuelo, Iker
Farran, Inmaculada
author Ancín, María
author_facet Ancín, María
Larraya, Luis
Florez-Sarasa, Igor
Bénard, Camille
Fernández-San Millán, Alicia
Veramendi, Jon
Gibon, Yves
Fernie, Alisdair R.
Aranjuelo, Iker
Farran, Inmaculada
author_role author
author2 Larraya, Luis
Florez-Sarasa, Igor
Bénard, Camille
Fernández-San Millán, Alicia
Veramendi, Jon
Gibon, Yves
Fernie, Alisdair R.
Aranjuelo, Iker
Farran, Inmaculada
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Vegetal
Genòmica i Biotecnologia
dc.subject.none.fl_str_mv 633
topic 633
description In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1443
https://doi.org/10.1093/jxb/erab193
url http://hdl.handle.net/20.500.12327/1443
https://doi.org/10.1093/jxb/erab193
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Experimental Botany
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/
EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM
MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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