Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age

Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include syn-thetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment...

Descripción completa

Detalles Bibliográficos
Autores: Nogacka, Alicja, Arboleya, Silvia, Nikpoor, Naghmeh, Auger, Jeremie, Salazar, Nuria, Cuesta Suárez, Isabel, Mantecón, Laura, Solís, Gonzalo, Gueimonde Fernández, Miguel, Tompkins, Thomas A., González de los Reyes-Gavilán, Clara
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/263558
Acceso en línea:http://hdl.handle.net/10261/263558
Access Level:acceso abierto
Palabra clave:Human milk oligosaccharides
2′-fucosyllactose
Microbiota
In vitro models
Infants
Breastfeeding
Bifidobacterium
id ES_b1a8ffc5b8b92abae2cee46d0db8547b
oai_identifier_str oai:digital.csic.es:10261/263558
network_acronym_str ES
network_name_str España
repository_id_str
spelling Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of ageNogacka, AlicjaArboleya, SilviaNikpoor, NaghmehAuger, JeremieSalazar, NuriaCuesta Suárez, IsabelMantecón, LauraSolís, GonzaloGueimonde Fernández, MiguelTompkins, Thomas A.González de los Reyes-Gavilán, ClaraHuman milk oligosaccharides2′-fucosyllactoseMicrobiotaIn vitro modelsInfantsBreastfeedingBifidobacteriumAlthough breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include syn-thetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment of the intestinal microbiota remains incompletely understood. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed, exclusively) in the presence of 2′fucosyllactose (2′FL), one of the most abundant HMOs in human milk. Microbiota composition was analyzed by 16S rRNA gene sequencing at baseline and at 24 h of incubation. The 2′FL consumption, gas accumulation, and levels of different metabolites were determined by chromatography. Microbiota profiles at baseline were clearly influenced by the mode of feeding and by the intrinsic ability of microbiotas to degrade 2′FL. The 2′FL degradation rate clustered fecal cultures into slow and fast degraders, regardless of feeding type, this being a determinant factor influencing the evolution of the microbiota during incubation, although the low number of donors precludes drawing sound conclusions. More studies are needed to decipher the extent to which the early intervention with HMOs could influence the microbiota as a function of its ability to utilize 2′FL.This research was funded by a contract of Lallemand Health Solutions Inc. with IPLA-CSIC and by the Intramural CSIC Research project PIE201970E061. S.A. was the recipient of a postdoctoral Juan de la Cierva Contract (Ministry of Science, Innovation and Universities, Ref. IJCI-2017-32156) and NS has a postdoctoral contract awarded by the Biosanitary Research Foundation in Asturias (FINBA, Spain).Molecular Diversity Preservation InternationalConsejo Superior de Investigaciones Científicas (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Fundación para la Investigación y la Innovación Biosanitaria del Principado de AsturiasConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/263558reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//IJCI-2017-32156http://dx.doi.org/10.3390/microorganisms9071478Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2635582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
title Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
spellingShingle Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
Nogacka, Alicja
Human milk oligosaccharides
2′-fucosyllactose
Microbiota
In vitro models
Infants
Breastfeeding
Bifidobacterium
title_short Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
title_full Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
title_fullStr Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
title_full_unstemmed Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
title_sort Influence of 2′-fucosyllactose on the microbiota composition and metabolic activity of fecal cultures from breastfed and formula-fed infants at two months of age
dc.creator.none.fl_str_mv Nogacka, Alicja
Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta Suárez, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde Fernández, Miguel
Tompkins, Thomas A.
González de los Reyes-Gavilán, Clara
author Nogacka, Alicja
author_facet Nogacka, Alicja
Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta Suárez, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde Fernández, Miguel
Tompkins, Thomas A.
González de los Reyes-Gavilán, Clara
author_role author
author2 Arboleya, Silvia
Nikpoor, Naghmeh
Auger, Jeremie
Salazar, Nuria
Cuesta Suárez, Isabel
Mantecón, Laura
Solís, Gonzalo
Gueimonde Fernández, Miguel
Tompkins, Thomas A.
González de los Reyes-Gavilán, Clara
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Fundación para la Investigación y la Innovación Biosanitaria del Principado de Asturias
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Human milk oligosaccharides
2′-fucosyllactose
Microbiota
In vitro models
Infants
Breastfeeding
Bifidobacterium
topic Human milk oligosaccharides
2′-fucosyllactose
Microbiota
In vitro models
Infants
Breastfeeding
Bifidobacterium
description Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include syn-thetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment of the intestinal microbiota remains incompletely understood. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed, exclusively) in the presence of 2′fucosyllactose (2′FL), one of the most abundant HMOs in human milk. Microbiota composition was analyzed by 16S rRNA gene sequencing at baseline and at 24 h of incubation. The 2′FL consumption, gas accumulation, and levels of different metabolites were determined by chromatography. Microbiota profiles at baseline were clearly influenced by the mode of feeding and by the intrinsic ability of microbiotas to degrade 2′FL. The 2′FL degradation rate clustered fecal cultures into slow and fast degraders, regardless of feeding type, this being a determinant factor influencing the evolution of the microbiota during incubation, although the low number of donors precludes drawing sound conclusions. More studies are needed to decipher the extent to which the early intervention with HMOs could influence the microbiota as a function of its ability to utilize 2′FL.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/263558
url http://hdl.handle.net/10261/263558
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//IJCI-2017-32156
http://dx.doi.org/10.3390/microorganisms9071478
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416950041411584
score 15,228081

Similares en LA Referencia