Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey

While artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with fro...

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Detalhes bibliográficos
Autores: Miró, Jordi, Catalán, Jaime, Marín, Henar, Yáñez-Ortiz, Iván, Yeste Oliveras, Marc
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/19534
Acesso em linha:http://hdl.handle.net/10256/19534
Access Level:acceso abierto
Palavra-chave:Ases -- Fecunditat
Donkeys -- Fertility
Ases -- Reproducció
Donkeys -- Reproduction
Inseminació artificial
Artificial insemination
Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
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oai_identifier_str oai:recercat.cat:10256/19534
network_acronym_str ES
network_name_str España
repository_id_str
spelling Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkeyMiró, JordiCatalán, JaimeMarín, HenarYáñez-Ortiz, IvánYeste Oliveras, MarcAses -- FecunditatDonkeys -- FertilityAses -- ReproduccióDonkeys -- ReproductionInseminació artificialArtificial inseminationAses -- EspermatozoidesDonkeys -- SpermatozoaEspermatozoidesSpermatozoaWhile artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with frozen-thawed sperm, as a high amount of polymorphonuclear neutrophils (PMN) are observed within the donkey uterus six hours after AI. While PMN appear to select the sperm that ultimately reach the oviduct, two mechanisms, phagocytosis and NETosis, have been purported to be involved in that clearance. Remarkably, sperm interacts with PMN, but the presence of seminal plasma reduces that binding. As seminal plasma is a complex fluid made up of different molecules, including proteins, this study aimed to evaluate how different seminal plasma fractions, separated by molecular weight (<3, 3-10, 10-30, 30-50, 50-100, and >100 kDa), affect sperm-PMN binding. Sperm motility, viability, and sperm-PMN binding were evaluated after 0 h, 1 h, 2 h, 3 h, and 4 h of co-incubation at 38 °C. Two seminal plasma fractions, including 30-50 kDa or 50-100 kDa proteins, showed the highest sperm motility and viability. As viability of sperm not bound to PMN after 3 h of incubation was the highest in the presence of 30-50 and 50-100 kDa proteins, we suggest that both fractions are involved in the control of the jenny's post-breeding inflammatory response. In conclusion, this study has shown for the first time that specific fractions rather than the entire seminal plasma modulate sperm-PMN binding within the donkey uterus. As several proteins suggested to be involved in the control of post-AI endometritis have a molecular weight between 30 and 100 kDa, further studies aimed at determining the identity of these molecules and evaluating their potential effect in vivo are much warrantedThis research was supported by the Ministry of Science and Innovation, Spain (Grants: RYC2014–15581 and AGL2017-88329-R), and the Regional Government of Catalonia (2017-SGR-1229). J.C. was funded by the National Agency for Research and Development (ANID), Ministry of Education, Chile (Scheme: Becas Chile Doctorado en el Extranjero, PFCHA; Grant: 2017/72180128). I.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019)Ministerio de Economía y Competitividad (Espanya)2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/19534http://hdl.handle.net/10256/19534Animals, 2021, vol. 11, núm. 5, p. 1388Articles publicats (D-B)Miró, Jordi Catalán, Jaime Marín, Henar Yáñez-Ortiz, Iván Yeste Oliveras, Marc 2021 Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey Animals 11 5 1388reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/ani11051388info:eu-repo/semantics/altIdentifier/eissn/2076-2615info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2014-15581Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/195342026-05-29T05:05:01Z
dc.title.none.fl_str_mv Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
title Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
spellingShingle Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
Miró, Jordi
Ases -- Fecunditat
Donkeys -- Fertility
Ases -- Reproducció
Donkeys -- Reproduction
Inseminació artificial
Artificial insemination
Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
title_short Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
title_full Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
title_fullStr Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
title_full_unstemmed Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
title_sort Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey
dc.creator.none.fl_str_mv Miró, Jordi
Catalán, Jaime
Marín, Henar
Yáñez-Ortiz, Iván
Yeste Oliveras, Marc
author Miró, Jordi
author_facet Miró, Jordi
Catalán, Jaime
Marín, Henar
Yáñez-Ortiz, Iván
Yeste Oliveras, Marc
author_role author
author2 Catalán, Jaime
Marín, Henar
Yáñez-Ortiz, Iván
Yeste Oliveras, Marc
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Ases -- Fecunditat
Donkeys -- Fertility
Ases -- Reproducció
Donkeys -- Reproduction
Inseminació artificial
Artificial insemination
Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
topic Ases -- Fecunditat
Donkeys -- Fertility
Ases -- Reproducció
Donkeys -- Reproduction
Inseminació artificial
Artificial insemination
Ases -- Espermatozoides
Donkeys -- Spermatozoa
Espermatozoides
Spermatozoa
description While artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with frozen-thawed sperm, as a high amount of polymorphonuclear neutrophils (PMN) are observed within the donkey uterus six hours after AI. While PMN appear to select the sperm that ultimately reach the oviduct, two mechanisms, phagocytosis and NETosis, have been purported to be involved in that clearance. Remarkably, sperm interacts with PMN, but the presence of seminal plasma reduces that binding. As seminal plasma is a complex fluid made up of different molecules, including proteins, this study aimed to evaluate how different seminal plasma fractions, separated by molecular weight (<3, 3-10, 10-30, 30-50, 50-100, and >100 kDa), affect sperm-PMN binding. Sperm motility, viability, and sperm-PMN binding were evaluated after 0 h, 1 h, 2 h, 3 h, and 4 h of co-incubation at 38 °C. Two seminal plasma fractions, including 30-50 kDa or 50-100 kDa proteins, showed the highest sperm motility and viability. As viability of sperm not bound to PMN after 3 h of incubation was the highest in the presence of 30-50 and 50-100 kDa proteins, we suggest that both fractions are involved in the control of the jenny's post-breeding inflammatory response. In conclusion, this study has shown for the first time that specific fractions rather than the entire seminal plasma modulate sperm-PMN binding within the donkey uterus. As several proteins suggested to be involved in the control of post-AI endometritis have a molecular weight between 30 and 100 kDa, further studies aimed at determining the identity of these molecules and evaluating their potential effect in vivo are much warranted
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/19534
http://hdl.handle.net/10256/19534
url http://hdl.handle.net/10256/19534
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ani11051388
info:eu-repo/semantics/altIdentifier/eissn/2076-2615
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Animals, 2021, vol. 11, núm. 5, p. 1388
Articles publicats (D-B)
Miró, Jordi Catalán, Jaime Marín, Henar Yáñez-Ortiz, Iván Yeste Oliveras, Marc 2021 Specific seminal plasma fractions are responsible for the modulation of sperm-PMN binding in the donkey Animals 11 5 1388
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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