Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses

Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins...

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Autores: Couté, Yohann, Hernandez, Céline, Ron D., Appel, Sanchez, Jean-Charles, Margolles Barros, Abelardo
Formato: artículo
Fecha de publicación:2007
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/7041
Acesso em linha:http://hdl.handle.net/10261/7041
Access Level:acceso abierto
Palavra-chave:Bifidobacterias
Leucina
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spelling Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic AnalysesCouté, YohannHernandez, CélineRon D., AppelSanchez, Jean-CharlesMargolles Barros, AbelardoBifidobacteriasLeucinaStable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidenceThis work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06727-C02-01/ALI and fellowship PR2006-0421) and by the Swiss National Science Foundation (grant 3100-A0-104214).Peer reviewedAmerican Society for MicrobiologyEuropean CommissionComisión Interministerial de Ciencia y Tecnología, CICYT (España)Swiss National Science Foundation200820082007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501144719 bytesapplication/pdfhttp://hdl.handle.net/10261/7041reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/70412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
title Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
spellingShingle Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
Couté, Yohann
Bifidobacterias
Leucina
title_short Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
title_full Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
title_fullStr Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
title_full_unstemmed Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
title_sort Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
dc.creator.none.fl_str_mv Couté, Yohann
Hernandez, Céline
Ron D., Appel
Sanchez, Jean-Charles
Margolles Barros, Abelardo
author Couté, Yohann
author_facet Couté, Yohann
Hernandez, Céline
Ron D., Appel
Sanchez, Jean-Charles
Margolles Barros, Abelardo
author_role author
author2 Hernandez, Céline
Ron D., Appel
Sanchez, Jean-Charles
Margolles Barros, Abelardo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Swiss National Science Foundation
dc.subject.none.fl_str_mv Bifidobacterias
Leucina
topic Bifidobacterias
Leucina
description Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence
publishDate 2007
dc.date.none.fl_str_mv 2007
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/7041
url http://hdl.handle.net/10261/7041
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 144719 bytes
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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