Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol

Introduction In vitro embryo production (IVP) in the domestic cat (Felis catus) remains highly variable owing to intrinsic reproductive traits and the absence of fully standardized protocols. Methods We retrospectively analysed 108 IVP replicates produced under a single protocol (2020–2024) to quant...

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Authors: Priego González, Andrea, Munoz-Maceda, Ana, Fuertes Recuero, Manuel, Medranda Ayjon, Paula, Sánchez-Calabuig, María Aránzazu, Barroso Arévalo, Sandra, Sánchez Calabuig, María Jesús
Format: article
Publication Date:2025
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/124606
Online Access:https://hdl.handle.net/20.500.14352/124606
Access Level:Open access
Keyword:636.082.4
Felis catus
Assisted reproductive technologies
Domestic cat
In vitro embryo production
Seasonality
Producción animal
3104 Producción Animal
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oai_identifier_str oai:docta.ucm.es:20.500.14352/124606
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repository_id_str
spelling Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocolPriego González, AndreaMunoz-Maceda, AnaFuertes Recuero, ManuelMedranda Ayjon, PaulaSánchez-Calabuig, María AránzazuBarroso Arévalo, SandraSánchez Calabuig, María Jesús636.082.4Felis catusAssisted reproductive technologiesDomestic catIn vitro embryo productionSeasonalityProducción animal3104 Producción AnimalIntroduction In vitro embryo production (IVP) in the domestic cat (Felis catus) remains highly variable owing to intrinsic reproductive traits and the absence of fully standardized protocols. Methods We retrospectively analysed 108 IVP replicates produced under a single protocol (2020–2024) to quantify the effects of season, donor age and methodological parameters on oocyte yield and embryo development. Results Winter proved the most favourable season for both oocyte recovery and blastocyst formation, whereas spring, despite lower initial yields, achieved the greatest post-selection oocyte retention. Donor age correlated negatively with oocyte number; however, older queens showed higher blastocyst conversion rates, suggesting that only developmentally competent oocytes persist at advanced age. Discussion These results highlight the need for rigorous donor selection and season-tailored IVP protocols to enhance embryo yield, quality and dataset reproducibility, prerequisites for deciphering embryo–maternal signalling mechanisms. Because the domestic cat serves as a valuable translational model for endangered felids, optimizing these factors will advance both feline ART and conservation breeding programs.Frontiers MediaUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124606reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1246062026-06-02T12:44:21Z
dc.title.none.fl_str_mv Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
title Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
spellingShingle Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
Priego González, Andrea
636.082.4
Felis catus
Assisted reproductive technologies
Domestic cat
In vitro embryo production
Seasonality
Producción animal
3104 Producción Animal
title_short Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
title_full Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
title_fullStr Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
title_full_unstemmed Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
title_sort Seasonal and age effects on in vitro embryo production in domestic cats under a standardized protocol
dc.creator.none.fl_str_mv Priego González, Andrea
Munoz-Maceda, Ana
Fuertes Recuero, Manuel
Medranda Ayjon, Paula
Sánchez-Calabuig, María Aránzazu
Barroso Arévalo, Sandra
Sánchez Calabuig, María Jesús
author Priego González, Andrea
author_facet Priego González, Andrea
Munoz-Maceda, Ana
Fuertes Recuero, Manuel
Medranda Ayjon, Paula
Sánchez-Calabuig, María Aránzazu
Barroso Arévalo, Sandra
Sánchez Calabuig, María Jesús
author_role author
author2 Munoz-Maceda, Ana
Fuertes Recuero, Manuel
Medranda Ayjon, Paula
Sánchez-Calabuig, María Aránzazu
Barroso Arévalo, Sandra
Sánchez Calabuig, María Jesús
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.082.4
Felis catus
Assisted reproductive technologies
Domestic cat
In vitro embryo production
Seasonality
Producción animal
3104 Producción Animal
topic 636.082.4
Felis catus
Assisted reproductive technologies
Domestic cat
In vitro embryo production
Seasonality
Producción animal
3104 Producción Animal
description Introduction In vitro embryo production (IVP) in the domestic cat (Felis catus) remains highly variable owing to intrinsic reproductive traits and the absence of fully standardized protocols. Methods We retrospectively analysed 108 IVP replicates produced under a single protocol (2020–2024) to quantify the effects of season, donor age and methodological parameters on oocyte yield and embryo development. Results Winter proved the most favourable season for both oocyte recovery and blastocyst formation, whereas spring, despite lower initial yields, achieved the greatest post-selection oocyte retention. Donor age correlated negatively with oocyte number; however, older queens showed higher blastocyst conversion rates, suggesting that only developmentally competent oocytes persist at advanced age. Discussion These results highlight the need for rigorous donor selection and season-tailored IVP protocols to enhance embryo yield, quality and dataset reproducibility, prerequisites for deciphering embryo–maternal signalling mechanisms. Because the domestic cat serves as a valuable translational model for endangered felids, optimizing these factors will advance both feline ART and conservation breeding programs.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124606
url https://hdl.handle.net/20.500.14352/124606
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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