Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering

n the present study, semi- and interpenetrated polymer network (IPN) systems based onhyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized andevaluated as potential...

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Autores: Mora-Boza, Ana, López-Ruiz, Elena, López-Donaire, M. L., Jiménez, Gema, Aguilar, María Rosa, Marchal, Juan A., Pedraz, José Luís, Vázquez-Lasa, Blanca, San Román, Julio, Gálvez Martín, Patricia
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/231631
Acesso em linha:http://hdl.handle.net/10261/231631
Access Level:Acceso aberto
Palavra-chave:interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
Chitosan
glycerylphytate
mesenchymal stem cel
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spelling Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineeringMora-Boza, AnaLópez-Ruiz, ElenaLópez-Donaire, M. L.Jiménez, GemaAguilar, María RosaMarchal, Juan A.Pedraz, José LuísVázquez-Lasa, BlancaSan Román, JulioGálvez Martín, Patriciainterpenetrated polymer networksemi-IPNmethacrylated hyaluronic acidChitosanglycerylphytatemesenchymal stem celn the present study, semi- and interpenetrated polymer network (IPN) systems based onhyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized andevaluated as potential supports for tissue engineering. Semi- and IPN systems showed significantdifferences between them regarding composition, morphology, and mechanical properties afterphysicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retentionand mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPNmembranes. The biological performance was evaluated on primary human mesenchymal stemcells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of thesystems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces.Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN,which suggested that these polymeric membranes could be proposed as an effective promoter systemof tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranescan provide a stable and amenable environment for hMSCs support and growth with potentialapplications in the biomedical field.Multidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/231631reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/polym12112661Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2316312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
title Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
spellingShingle Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
Mora-Boza, Ana
interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
Chitosan
glycerylphytate
mesenchymal stem cel
title_short Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
title_full Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
title_fullStr Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
title_full_unstemmed Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
title_sort Evaluation of glycerylphytate crosslinked semi- and interpenetrated polymer membranes of hyaluronic acid and chitosan for tissue engineering
dc.creator.none.fl_str_mv Mora-Boza, Ana
López-Ruiz, Elena
López-Donaire, M. L.
Jiménez, Gema
Aguilar, María Rosa
Marchal, Juan A.
Pedraz, José Luís
Vázquez-Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author Mora-Boza, Ana
author_facet Mora-Boza, Ana
López-Ruiz, Elena
López-Donaire, M. L.
Jiménez, Gema
Aguilar, María Rosa
Marchal, Juan A.
Pedraz, José Luís
Vázquez-Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author_role author
author2 López-Ruiz, Elena
López-Donaire, M. L.
Jiménez, Gema
Aguilar, María Rosa
Marchal, Juan A.
Pedraz, José Luís
Vázquez-Lasa, Blanca
San Román, Julio
Gálvez Martín, Patricia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
Chitosan
glycerylphytate
mesenchymal stem cel
topic interpenetrated polymer network
semi-IPN
methacrylated hyaluronic acid
Chitosan
glycerylphytate
mesenchymal stem cel
description n the present study, semi- and interpenetrated polymer network (IPN) systems based onhyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized andevaluated as potential supports for tissue engineering. Semi- and IPN systems showed significantdifferences between them regarding composition, morphology, and mechanical properties afterphysicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retentionand mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPNmembranes. The biological performance was evaluated on primary human mesenchymal stemcells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of thesystems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces.Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN,which suggested that these polymeric membranes could be proposed as an effective promoter systemof tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranescan provide a stable and amenable environment for hMSCs support and growth with potentialapplications in the biomedical field.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/231631
url http://hdl.handle.net/10261/231631
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/polym12112661
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416538553974784
score 15.812455

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