Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10...

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Autores: Delaby, C, Munoz, L, Torres, S, Nadal, A, Le Bastard, N, Lehmann, S, Lleo, A, Alcolea, D
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositório:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p2966
Acesso em linha:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2966
https://hal.umontpellier.fr/hal-02626119
Access Level:Acceso aberto
Palavra-chave:Cerebrospinal fluid
Biomarkers
Amyloid
Tau
Lumipulse
Alzheimer's disease
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spelling Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platformDelaby, CMunoz, LTorres, SNadal, ALe Bastard, NLehmann, SLleo, AAlcolea, DCerebrospinal fluidBiomarkersAmyloidTauLumipulseAlzheimer's diseaseObjectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF A beta 1-42, A beta 1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF A beta 1-42 and A beta 1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio A beta 1-42/A beta 1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.ELSEVIER2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2966https://hal.umontpellier.fr/hal-02626119CLINICA CHIMICA ACTAISSN: 00098981ISSNe: 18733492reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p29662026-06-14T12:41:47Z
dc.title.none.fl_str_mv Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
title Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
spellingShingle Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
Delaby, C
Cerebrospinal fluid
Biomarkers
Amyloid
Tau
Lumipulse
Alzheimer's disease
title_short Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
title_full Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
title_fullStr Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
title_full_unstemmed Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
title_sort Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform
dc.creator.none.fl_str_mv Delaby, C
Munoz, L
Torres, S
Nadal, A
Le Bastard, N
Lehmann, S
Lleo, A
Alcolea, D
author Delaby, C
author_facet Delaby, C
Munoz, L
Torres, S
Nadal, A
Le Bastard, N
Lehmann, S
Lleo, A
Alcolea, D
author_role author
author2 Munoz, L
Torres, S
Nadal, A
Le Bastard, N
Lehmann, S
Lleo, A
Alcolea, D
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerebrospinal fluid
Biomarkers
Amyloid
Tau
Lumipulse
Alzheimer's disease
topic Cerebrospinal fluid
Biomarkers
Amyloid
Tau
Lumipulse
Alzheimer's disease
description Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF A beta 1-42, A beta 1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF A beta 1-42 and A beta 1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio A beta 1-42/A beta 1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2966
https://hal.umontpellier.fr/hal-02626119
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2966
https://hal.umontpellier.fr/hal-02626119
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv CLINICA CHIMICA ACTA
ISSN: 00098981
ISSNe: 18733492
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
repository.name.fl_str_mv
repository.mail.fl_str_mv
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