Bacterial antisense RNAs are mainly the product of transcriptional noise

cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 ba...

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Autores: Lloréns Rico, Verónica, 1989-, Cano, Jaime, Kamminga, Tjerko, Gil, Rosario, Latorre, Amparo, Chen, Wei-Hua, Bork, Peer, Glass, John I., Serrano Pubull, Luis, 1982-, Lluch-Senar, Maria 1982-
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/30911
Acesso em linha:http://hdl.handle.net/10230/30911
http://dx.doi.org/10.1126/sciadv.1501363
Access Level:acceso abierto
Palavra-chave:RNA
Bacterial antisense RNAs
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spelling Bacterial antisense RNAs are mainly the product of transcriptional noiseLloréns Rico, Verónica, 1989-Cano, JaimeKamminga, TjerkoGil, RosarioLatorre, AmparoChen, Wei-HuaBork, PeerGlass, John I.Serrano Pubull, Luis, 1982-Lluch-Senar, Maria 1982-RNABacterial antisense RNAscis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asRNAs is exponentially dependent on the genomic AT content and that expression of asRNA at low levels exerts little impact in terms of energy consumption. A transcription model simulating mRNA and asRNA production indicates that the asRNA regulatory effect is only observed above certain expression thresholds, substantially higher than physiological transcript levels. These predictions were verified experimentally by overexpressing nine different asRNAs in Mycoplasma pneumoniae. Our results suggest that most of the antisense transcripts found in bacteria are the consequence of transcriptional noise, arising at spurious promoters throughout the genome.This work was supported by the European Union Seventh Framework Programme (FP7/2007–2013), through the European Research Council (232913); Fundación Botín, the Spanish Ministry of Economy and Competitiveness (BIO2007-61762); National Plan of R + D + i; ISCIII—Subdirección General de Evaluación y Fomento de la Investigación (PI10/01702); European Regional Development Fund (to the Institució Catalana de Recerca i Estudis Avançats research professor L.S.); and Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017” (SEV-2012-0208). A.L. received grant BFU2012-39816-C02-01 from the Spanish Ministry of Economy and Competitivity cofinanced by FEDER (Fondo Europeo de Desarrollo Regional) funds.American Association for the Advancement of Science (AAAS)201720172016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/30911http://dx.doi.org/10.1126/sciadv.1501363reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésScience Advances. 2016;2(3):e150363info:eu-repo/grantAgreement/EC/FP7/232913Copyright © 2016, The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. DOI: 10.1126/sciadv.1501363https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/309112026-05-29T05:05:01Z
dc.title.none.fl_str_mv Bacterial antisense RNAs are mainly the product of transcriptional noise
title Bacterial antisense RNAs are mainly the product of transcriptional noise
spellingShingle Bacterial antisense RNAs are mainly the product of transcriptional noise
Lloréns Rico, Verónica, 1989-
RNA
Bacterial antisense RNAs
title_short Bacterial antisense RNAs are mainly the product of transcriptional noise
title_full Bacterial antisense RNAs are mainly the product of transcriptional noise
title_fullStr Bacterial antisense RNAs are mainly the product of transcriptional noise
title_full_unstemmed Bacterial antisense RNAs are mainly the product of transcriptional noise
title_sort Bacterial antisense RNAs are mainly the product of transcriptional noise
dc.creator.none.fl_str_mv Lloréns Rico, Verónica, 1989-
Cano, Jaime
Kamminga, Tjerko
Gil, Rosario
Latorre, Amparo
Chen, Wei-Hua
Bork, Peer
Glass, John I.
Serrano Pubull, Luis, 1982-
Lluch-Senar, Maria 1982-
author Lloréns Rico, Verónica, 1989-
author_facet Lloréns Rico, Verónica, 1989-
Cano, Jaime
Kamminga, Tjerko
Gil, Rosario
Latorre, Amparo
Chen, Wei-Hua
Bork, Peer
Glass, John I.
Serrano Pubull, Luis, 1982-
Lluch-Senar, Maria 1982-
author_role author
author2 Cano, Jaime
Kamminga, Tjerko
Gil, Rosario
Latorre, Amparo
Chen, Wei-Hua
Bork, Peer
Glass, John I.
Serrano Pubull, Luis, 1982-
Lluch-Senar, Maria 1982-
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA
Bacterial antisense RNAs
topic RNA
Bacterial antisense RNAs
description cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asRNAs is exponentially dependent on the genomic AT content and that expression of asRNA at low levels exerts little impact in terms of energy consumption. A transcription model simulating mRNA and asRNA production indicates that the asRNA regulatory effect is only observed above certain expression thresholds, substantially higher than physiological transcript levels. These predictions were verified experimentally by overexpressing nine different asRNAs in Mycoplasma pneumoniae. Our results suggest that most of the antisense transcripts found in bacteria are the consequence of transcriptional noise, arising at spurious promoters throughout the genome.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/30911
http://dx.doi.org/10.1126/sciadv.1501363
url http://hdl.handle.net/10230/30911
http://dx.doi.org/10.1126/sciadv.1501363
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Science Advances. 2016;2(3):e150363
info:eu-repo/grantAgreement/EC/FP7/232913
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for the Advancement of Science (AAAS)
publisher.none.fl_str_mv American Association for the Advancement of Science (AAAS)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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