RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

19 pages, 7 figures, 3 tables

Detalles Bibliográficos
Autores: Romero, Mónica R., Pérez-Figueroa, Andrés, Carrera, Mónica, Swanson, Willie J., Skibinski, David O. F., Diz, Ángel P.
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/176257
Acceso en línea:http://hdl.handle.net/10261/176257
Access Level:acceso abierto
Palabra clave:Sperm
Gonad
External fertilisation
Marine invertebrates
Reproductive isolation
Speciation
Proteomics
Transcriptomics
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spelling RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel speciesRomero, Mónica R.Pérez-Figueroa, AndrésCarrera, MónicaSwanson, Willie J.Skibinski, David O. F.Diz, Ángel P.SpermGonadExternal fertilisationMarine invertebratesReproductive isolationSpeciationProteomicsTranscriptomics19 pages, 7 figures, 3 tablesSpeciation mechanisms in marine organisms have attracted great interest because of the apparent lack of substantial barriers to genetic exchange in marine ecosystems. Marinemussels of the Mytilus edulis species complex provide a good model to study mechanisms underlying species formation. They hybridise extensively at many localities and both pre- and postzygotic isolating mechanisms may be operating. Mussels have external fertilisation and sperm cells should show specific adaptations for survival and successful fertilisation. Sperm thus represent key targets in investigations of the molecular mechanisms underlying reproductive isolation. We undertook a deep transcriptome sequencing (RNA-seq) of mature male gonads and a 2DE/MS-based proteome analysis of sperm from Mytilus edulis and M. galloprovincialis raised in a common environment. We provide evidence of extensive expression differences between the two mussel species, and general agreement between the transcriptomic and proteomic results in the direction of expression differences between species. Differential expression is marked for mitochondrial genes and for those involved in spermatogenesis, sperm motility, sperm-egg interactions, the acrosome reaction, sperm capacitation, ATP reserves and ROS production. Proteins and their corresponding genes might thus be good targets in further genomic analysis of reproductive barriers between these closely related speciesThis work was funded by the Spanish “Ministerio de Economía y Competitividad” (codes BFU2011-22599 and AGL2014-52062-R)”, Fondos Feder (ERDF, European Commission) and Xunta de Galicia (“Grupos de Referencia Competitiva” ED431C 2016-037), and NIH grant HD076862. M.R. Romero was supported by a predoctoral fellowship from Xunta de Galicia (Campus do Mar)Peer reviewedElsevierMinisterio de Economía y Competitividad (España)European CommissionXunta de GaliciaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/176257reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//BFU2011-22599The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.1016/j.jprot.2018.08.020https://doi.org/10.1016/j.jprot.2018.08.020Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1762572026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
spellingShingle RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
Romero, Mónica R.
Sperm
Gonad
External fertilisation
Marine invertebrates
Reproductive isolation
Speciation
Proteomics
Transcriptomics
title_short RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_full RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_fullStr RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_full_unstemmed RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
title_sort RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species
dc.creator.none.fl_str_mv Romero, Mónica R.
Pérez-Figueroa, Andrés
Carrera, Mónica
Swanson, Willie J.
Skibinski, David O. F.
Diz, Ángel P.
author Romero, Mónica R.
author_facet Romero, Mónica R.
Pérez-Figueroa, Andrés
Carrera, Mónica
Swanson, Willie J.
Skibinski, David O. F.
Diz, Ángel P.
author_role author
author2 Pérez-Figueroa, Andrés
Carrera, Mónica
Swanson, Willie J.
Skibinski, David O. F.
Diz, Ángel P.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Xunta de Galicia
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sperm
Gonad
External fertilisation
Marine invertebrates
Reproductive isolation
Speciation
Proteomics
Transcriptomics
topic Sperm
Gonad
External fertilisation
Marine invertebrates
Reproductive isolation
Speciation
Proteomics
Transcriptomics
description 19 pages, 7 figures, 3 tables
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/176257
url http://hdl.handle.net/10261/176257
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//BFU2011-22599
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.1016/j.jprot.2018.08.020
https://doi.org/10.1016/j.jprot.2018.08.020
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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