Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence

Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is...

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Autores: Ramírez, Laura, de Moura, Luana Dias, Mateus, Natalia Lopes Fontoura, de Moraes, Milene Hoehr, do Nascimento, Leopoldo Fabrício Marçal, de Jesus Melo, Nailson, Taketa, Lucas Bezerra, Catecati, Tatiana, Huete, Samuel G., Penichet, Karla, Piranda, Eliane Mattos, de Oliveira, Alessandra Gutierrez, Steindel, Mario, Barral-Netto, Manoel, do Socorro Pires e Cruz, Maria, Barral, Aldina, Soto Álvarez, Manuel
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/691480
Acesso em linha:http://hdl.handle.net/10486/691480
https://dx.doi.org/10.1016/j.parepi.2019.e00126
Access Level:acceso abierto
Palavra-chave:Antibodies
Canine leishmaniasis
Leishmania
Recombinant proteins
Serodiagnosis
Biología y Biomedicina / Biología
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spelling Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalenceRamírez, Laurade Moura, Luana DiasMateus, Natalia Lopes Fontourade Moraes, Milene Hoehrdo Nascimento, Leopoldo Fabrício Marçalde Jesus Melo, NailsonTaketa, Lucas BezerraCatecati, TatianaHuete, Samuel G.Penichet, KarlaPiranda, Eliane Mattosde Oliveira, Alessandra GutierrezSteindel, MarioBarral-Netto, Manoeldo Socorro Pires e Cruz, MariaBarral, AldinaSoto Álvarez, ManuelAntibodiesCanine leishmaniasisLeishmaniaRecombinant proteinsSerodiagnosisBiología y Biomedicina / BiologíaSerodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with subclinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extractsThis work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil) within the call“CNPq/MS/SCTIE/DECIT N° 32/2014 - Pesquisas sobre Leishmanioses”grant number reference 467389/2014-4. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. TC received scholarship from Fundação de Amparo a Pesquisa do Estado de Santa Catarina–FAPESCElsevier BVDepartamento de Biología MolecularFacultad de Ciencias20202020-02-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/691480https://dx.doi.org/10.1016/j.parepi.2019.e00126reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6914802026-06-23T12:46:27Z
dc.title.none.fl_str_mv Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
title Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
spellingShingle Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
Ramírez, Laura
Antibodies
Canine leishmaniasis
Leishmania
Recombinant proteins
Serodiagnosis
Biología y Biomedicina / Biología
title_short Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
title_full Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
title_fullStr Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
title_full_unstemmed Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
title_sort Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
dc.creator.none.fl_str_mv Ramírez, Laura
de Moura, Luana Dias
Mateus, Natalia Lopes Fontoura
de Moraes, Milene Hoehr
do Nascimento, Leopoldo Fabrício Marçal
de Jesus Melo, Nailson
Taketa, Lucas Bezerra
Catecati, Tatiana
Huete, Samuel G.
Penichet, Karla
Piranda, Eliane Mattos
de Oliveira, Alessandra Gutierrez
Steindel, Mario
Barral-Netto, Manoel
do Socorro Pires e Cruz, Maria
Barral, Aldina
Soto Álvarez, Manuel
author Ramírez, Laura
author_facet Ramírez, Laura
de Moura, Luana Dias
Mateus, Natalia Lopes Fontoura
de Moraes, Milene Hoehr
do Nascimento, Leopoldo Fabrício Marçal
de Jesus Melo, Nailson
Taketa, Lucas Bezerra
Catecati, Tatiana
Huete, Samuel G.
Penichet, Karla
Piranda, Eliane Mattos
de Oliveira, Alessandra Gutierrez
Steindel, Mario
Barral-Netto, Manoel
do Socorro Pires e Cruz, Maria
Barral, Aldina
Soto Álvarez, Manuel
author_role author
author2 de Moura, Luana Dias
Mateus, Natalia Lopes Fontoura
de Moraes, Milene Hoehr
do Nascimento, Leopoldo Fabrício Marçal
de Jesus Melo, Nailson
Taketa, Lucas Bezerra
Catecati, Tatiana
Huete, Samuel G.
Penichet, Karla
Piranda, Eliane Mattos
de Oliveira, Alessandra Gutierrez
Steindel, Mario
Barral-Netto, Manoel
do Socorro Pires e Cruz, Maria
Barral, Aldina
Soto Álvarez, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Antibodies
Canine leishmaniasis
Leishmania
Recombinant proteins
Serodiagnosis
Biología y Biomedicina / Biología
topic Antibodies
Canine leishmaniasis
Leishmania
Recombinant proteins
Serodiagnosis
Biología y Biomedicina / Biología
description Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with subclinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extracts
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-02-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/691480
https://dx.doi.org/10.1016/j.parepi.2019.e00126
url http://hdl.handle.net/10486/691480
https://dx.doi.org/10.1016/j.parepi.2019.e00126
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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