Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata

This article is part of the Research Topic Recent Advances on Grapevine-Microbe Interactions: From Signal Perception to Resistance Response

Detalles Bibliográficos
Autores: Cobos Román, Rebeca, Calvo Peña, Carla, Álvarez Pérez, José Manuel, Ibáñez Sánchez, Ana María, Diez Galán, Alba, González García, Sandra, García Angulo, Penélope, Acebes Arranz, José Luis, Rubio Coque, Juan José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18938
Acceso en línea:https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01282
https://hdl.handle.net/10612/18938
Access Level:acceso abierto
Palabra clave:Biología
Botryosphaeriaceae
NLP
RT-qPCR
Grapevine trunk diseases
Phytotoxicity
Vitis vinifera
3108 Fitopatología
2414 Microbiología
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spelling Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriataCobos Román, RebecaCalvo Peña, CarlaÁlvarez Pérez, José ManuelIbáñez Sánchez, Ana MaríaDiez Galán, AlbaGonzález García, SandraGarcía Angulo, PenélopeAcebes Arranz, José LuisRubio Coque, Juan JoséBiologíaBotryosphaeriaceaeNLPRT-qPCRGrapevine trunk diseasesPhytotoxicityVitis vinifera3108 Fitopatología2414 MicrobiologíaThis article is part of the Research Topic Recent Advances on Grapevine-Microbe Interactions: From Signal Perception to Resistance Response[EN] Many phytopathogenic fungi produce necrosis and ethylene inducing peptide 1 (Nep1- like proteins or NLP) that trigger leaf necrosis and the activation of defense mechanisms. These proteins have been widely studied in plant pathogens as Moniliophthora perniciosa or Botrytis cinerea between others, but little is known about their biological roles in grapevine trunk pathogens. Advances in the sequencing of genomes of several fungi involved in grapevine trunk diseases have revealed that these proteins are present in several copies in their genomes. The aim of this project was to analyze the presence of genes encoding NLP proteins in the Diplodia seriata genome and to characterize their putative role as virulence factors associated to grapevine trunk diseases. In this study, we characterized four NLPs from Diplodia seriata. All proteins showed highly similar amino acid sequences and contained the characteristic peptide motifs of NLPs. DserNEPs slightly reduced the viability of Vitis vinifera L. cell cultures. The cytolytic activity from DserNEP1 was stronger than that from DserNEP2, even at low concentrations. Purified DserNEPs also produced necrosis in leaves when they were inoculated into micropropagules of V. vinifera L. This is the first record of Nep1-like proteins from a fungus associated with grapevine trunk diseases and also from a member of the Botryosphaeriaceae familySIThis work was supported by Bodegas Vega Sicilia S.A. (Valbuena de Duero, Valladolid, Spain)FrontiersMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01282https://hdl.handle.net/10612/18938reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/189382026-06-24T12:43:27Z
dc.title.none.fl_str_mv Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
title Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
spellingShingle Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
Cobos Román, Rebeca
Biología
Botryosphaeriaceae
NLP
RT-qPCR
Grapevine trunk diseases
Phytotoxicity
Vitis vinifera
3108 Fitopatología
2414 Microbiología
title_short Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
title_full Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
title_fullStr Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
title_full_unstemmed Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
title_sort Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
dc.creator.none.fl_str_mv Cobos Román, Rebeca
Calvo Peña, Carla
Álvarez Pérez, José Manuel
Ibáñez Sánchez, Ana María
Diez Galán, Alba
González García, Sandra
García Angulo, Penélope
Acebes Arranz, José Luis
Rubio Coque, Juan José
author Cobos Román, Rebeca
author_facet Cobos Román, Rebeca
Calvo Peña, Carla
Álvarez Pérez, José Manuel
Ibáñez Sánchez, Ana María
Diez Galán, Alba
González García, Sandra
García Angulo, Penélope
Acebes Arranz, José Luis
Rubio Coque, Juan José
author_role author
author2 Calvo Peña, Carla
Álvarez Pérez, José Manuel
Ibáñez Sánchez, Ana María
Diez Galán, Alba
González García, Sandra
García Angulo, Penélope
Acebes Arranz, José Luis
Rubio Coque, Juan José
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Botryosphaeriaceae
NLP
RT-qPCR
Grapevine trunk diseases
Phytotoxicity
Vitis vinifera
3108 Fitopatología
2414 Microbiología
topic Biología
Botryosphaeriaceae
NLP
RT-qPCR
Grapevine trunk diseases
Phytotoxicity
Vitis vinifera
3108 Fitopatología
2414 Microbiología
description This article is part of the Research Topic Recent Advances on Grapevine-Microbe Interactions: From Signal Perception to Resistance Response
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01282
https://hdl.handle.net/10612/18938
url https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01282
https://hdl.handle.net/10612/18938
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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