GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular...

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Autores: Llavanera, Marc, Delgado Bermúdez, Ariadna, Fernández Fuertes, Beatriz, Recuero, Sandra, Mateo Otero, Yentel, Bonet, Sergi, Barranco, Isabel, Yeste Oliveras, Marc
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18218
Acesso em linha:http://hdl.handle.net/10256/18218
Access Level:acceso abierto
Palavra-chave:Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
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oai_identifier_str oai:recercat.cat:10256/18218
network_acronym_str ES
network_name_str España
repository_id_str
spelling GSTM3, but not IZUMO1, is a cryotolerance marker of boar spermLlavanera, MarcDelgado Bermúdez, AriadnaFernández Fuertes, BeatrizRecuero, SandraMateo Otero, YentelBonet, SergiBarranco, IsabelYeste Oliveras, MarcPorcs -- Espermatozoides -- InvestigacióSwine -- Spermatozoa -- ResearchSenglar -- Espermatozoides -- CriopreservacióWild boar -- Spermatozoa -- CryopreservationSenglar -- EspermatozoidesWild boar -- SpermatozoaCryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction. Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm. However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor (PFE) than in good (GFE) freezability ejaculates in both pre-frozen (1.00 INT·mm2 ± 0.14 INT·mm2 vs. 0.72 INT·mm2 ± 0.15 INT·mm2 ; P < 0.05) and post-thawed (0.96 INT·mm2 ± 0.20 INT·mm2 vs. 70 INT·mm2 ± 0.19 INT·mm2 ; P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species (ROS) production, and high membrane lipid disorder postthaw (P < 0.05). Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar spermThe authors acknowledge the support from Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581, AGL2017–88329-R and FJCI2017-31689) and European Commission (H2020-MSCA-IF-79212)BioMed CentralAgencia Estatal de InvestigaciónMinisterio de Economía y Competitividad (Espanya)2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/18218Journal of Animal Science and Biotechnology, 2019, vol. 10, p. 61Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40104-019-0370-5info:eu-repo/semantics/altIdentifier/eissn/2049-1891info:eu-repo/grantAgreement/MINECO//RYC-2014-15581info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-RAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/182182026-05-29T05:05:01Z
dc.title.none.fl_str_mv GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
title GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
spellingShingle GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
Llavanera, Marc
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
title_short GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
title_full GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
title_fullStr GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
title_full_unstemmed GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
title_sort GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
dc.creator.none.fl_str_mv Llavanera, Marc
Delgado Bermúdez, Ariadna
Fernández Fuertes, Beatriz
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Yeste Oliveras, Marc
author Llavanera, Marc
author_facet Llavanera, Marc
Delgado Bermúdez, Ariadna
Fernández Fuertes, Beatriz
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Yeste Oliveras, Marc
author_role author
author2 Delgado Bermúdez, Ariadna
Fernández Fuertes, Beatriz
Recuero, Sandra
Mateo Otero, Yentel
Bonet, Sergi
Barranco, Isabel
Yeste Oliveras, Marc
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
topic Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Senglar -- Espermatozoides -- Criopreservació
Wild boar -- Spermatozoa -- Cryopreservation
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
description Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction. Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm. However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor (PFE) than in good (GFE) freezability ejaculates in both pre-frozen (1.00 INT·mm2 ± 0.14 INT·mm2 vs. 0.72 INT·mm2 ± 0.15 INT·mm2 ; P < 0.05) and post-thawed (0.96 INT·mm2 ± 0.20 INT·mm2 vs. 70 INT·mm2 ± 0.19 INT·mm2 ; P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species (ROS) production, and high membrane lipid disorder postthaw (P < 0.05). Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/18218
url http://hdl.handle.net/10256/18218
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s40104-019-0370-5
info:eu-repo/semantics/altIdentifier/eissn/2049-1891
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Journal of Animal Science and Biotechnology, 2019, vol. 10, p. 61
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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